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公开(公告)号：US11697685B2
公开(公告)日：2023-07-11
申请号：US16700401
申请日：2019-12-02
发明人： Huashun Li , Baolei Wang , Kunkun Han
IPC分类号： C07K16/28 , C07K14/705 , C12N5/0783 , A61K35/17 , A61P35/00 , C07K14/725 , C12N15/867 , A61K38/00 , A61K39/00
CPC分类号： C07K16/2803 , A61K35/17 , A61P35/00 , C07K14/7051 , C07K14/70517 , C07K14/70521 , C07K14/70578 , C12N5/0646 , C12N15/867 , A61K38/00 , A61K2039/505 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/76 , C07K2319/02 , C07K2319/03 , C07K2319/30 , C07K2319/33 , C12N5/0636 , C12N2510/00
摘要： The present invention relates to chimeric antigen receptor cells targeting ROBO1, in particular, enhanced CAR-T cells and CAR-NK cells targeting ROBO1, and preparation and application thereof. The cells can stably expressing CAR elements, while secreting extracellular domain molecules expressing PD-1 protein or mutants thereof, and thus may block PD-11PD-L1 molecular interaction. It has been found through animal experiments that the cells have very good anti-tumor effects, and the above-mentioned cells can significantly reduce tumor recurrence and improve the survival rate compared with the conventional ROBO1-targeted CAR modified cells.
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公开(公告)号：US11331345B2
公开(公告)日：2022-05-17
申请号：US16389954
申请日：2019-04-21
发明人： Huashun Li , Baolei Wang , Kunkun Han , Baoyong Ren
IPC分类号： A61K35/17 , C07K14/47 , A61P35/00 , C12N15/86 , A61K48/00 , C12N15/62 , C12N15/867 , C12N5/10 , C07K19/00
摘要： Provided are a PD-1 CAR NK-92 cell and a preparation method and use thereof. The PD-1 CAR NK-92 cell expresses PD-1-CD8™-4-1BB-CD3ζ fusion protein in NK-92 cells. The PD-1 CAR NK-92 is obtained by infecting an NK92 cell line with a PD-1 CAR molecule and obtaining monoclonal cells by means of flow screening, and culturing and expanding CAR NK92 monoclonal cell strains with stable traits and a high killing activity. The cells can be produced on a large scale, can be used in different patients without GVHR rejection, and have a specific killing activity and significant therapeutic effect on tumors.
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公开(公告)号：US11246888B2
公开(公告)日：2022-02-15
申请号：US16160735
申请日：2018-10-15
发明人： Huashun Li , Baolei Wang , Baoyong Ren
IPC分类号： A61K35/17 , C07K14/705 , C07K19/00 , C12N15/09 , A61P35/00 , C07K14/725 , C12N15/85 , C12N5/10 , C07K14/78 , C07K14/73
摘要： A chimeric antigen receptor (CAR) and a gene encoding the CAR. The CAR comprises an extracellular domain capable of binding to an antigen, a transmembrane domain, and intracellular immune co-stimulatory molecule, wherein the extracellular domain comprises a D2 domain of a Slit2 protein. A chimeric antibody-expressing cell, which introduces a gene encoding the CAR into a cell so as to express the CAR on the surface of the cell. The CAR or CAR-expressing cell can be used as a cell drug for the treatment of tumor diseases. By using the CAR for engineering cells, especially T cells, the engineered T cells can specifically recognize and kill tumors, and have higher tumoricidal activity.
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公开(公告)号：US11738051B2
公开(公告)日：2023-08-29
申请号：US16893989
申请日：2020-06-05
发明人： Huashun Li , Kunkun Han , Baolei Wang , Baoyong Ren
IPC分类号： A61K35/17 , A61P35/00 , C07K14/725 , C07K14/705 , C12N15/62
CPC分类号： A61K35/17 , A61P35/00 , C07K14/7051 , C07K14/70517 , C07K14/70578 , C12N15/62
摘要： The present invention provides a nucleotide sequence for encoding CAR, a ROBO1 CAR-NK cell of expressing the CAR, and preparation and application thereof. The ROBO1 CAR-NK cell provided by the present invention can specifically kill tumor cells by using ROBO1 antibody for the construction of CAR-NK cells and using ROBO1 molecules as target antigens. It can be used as a therapeutic agent for tumor diseases, for the treatment of tumor with highly expressing of ROBO1, without harmful phenomena such as cytokine release syndrome, thus providing new treatments for the tumors which are ineffective in traditional surgery, chemotherapy and radiotherapy. It has lower toxicity, higher safety and better specific lysis activity compared with ROBO1 CAR-T cell.
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