公开(公告)号：US5386112A

公开(公告)日：1995-01-31

申请号：US995989

申请日：1992-12-23

申请人： Arthur E. Dixon

发明人： Arthur E. Dixon

IPC分类号： G02B21/00 ,  H01J3/14

CPC分类号： G02B21/0068 ,  G02B21/0052 ,  G02B21/0056 ,  G02B21/0072 ,  G02B21/0076

摘要： A new scanning optical microscope or mapping system for transmission, reflection and luminescence imaging is disclosed. A transmission arm collects light transmitted through the specimen and injects it back into the optical path of the microscope parallel with and coaxial with the reflected light beam returning from the specimen, so both transmitted and reflected light beams are descanned by the same scan optics on their way back to the same detector. Since light travels around the transmission arm in both directions, this microscope forms images of the specimen from either the top or the bottom and in either reflected light or transmitted light, and all four of these imaging modes can be selected separately. Embodiments for use in fluorescence and photoluminescence imaging are also disclosed.

摘要翻译： 公开了一种用于透射，反射和发光成像的新型扫描光学显微镜或测绘系统。 透射臂收集穿过样本的光，并将其再次注入到与样本反射的反射光束平行并与其同轴的显微镜的光路中，因此透射和反射的光束被它们的相同的扫描光学器件 回到相同的检测器。 由于光在两个方向上绕传动臂传播，因此该显微镜从顶部或底部以及反射光或透射光中形成样本的图像，并且可以分开选择所有这四种成像模式。 还公开了用于荧光和光致发光成像的实施方案。

公开(公告)号：US20110064296A1

公开(公告)日：2011-03-17

申请号：US12993010

申请日：2009-05-15

申请人： Arthur E. Dixon

发明人： Arthur E. Dixon

IPC分类号： G06K9/00

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G02B21/0024 ,  G02B21/16 ,  G02B21/367

摘要： A method of operating an instrument that is a macroscope, microscope, or slide scanner is provided where the instrument has a larger dynamic range for measurement than a dynamic range required in the final image of a specimen. In the method, data is measured from a specimen using the instrument, and the dynamic range of the measured data is contracted in the final image file during scanning.

摘要翻译： 提供操作作为宏观显微镜，显微镜或幻灯片扫描器的仪器的方法，其中仪器具有比测试的最终图像中所需的动态范围更大的测量动态范围。 在该方法中，使用仪器从样本测量数据，并且在扫描期间将测量数据的动态范围收缩在最终图像文件中。

公开(公告)号：US5381224A

公开(公告)日：1995-01-10

申请号：US113172

申请日：1993-08-30

申请人： Arthur E. Dixon ,  Savvas Damaskinos

发明人： Arthur E. Dixon ,  Savvas Damaskinos

IPC分类号： G01N21/64

CPC分类号： G01N21/6456

摘要： A scanning optical imaging or mapping system for macroscopic specimens is disclosed, which allows both confocal and non-confocal imaging to be performed in reflected light, photoluminescence, fluorescence and other contrast mechanisms. Several embodiments are disclosed, for use in photoluminescence mapping of semiconductor specimens, fluorescence scanning of gels used in gene sequencing, fingerprint detection, and other application areas.

摘要翻译： 公开了一种用于宏观样本的扫描光学成像或映射系统，其允许在反射光，光致发光，荧光和其它对比度机制中执行共焦和非共焦成像。 公开了几种实施方案，用于半导体样品的光致发光测定，用于基因测序的凝胶的荧光扫描，指纹检测和其它应用领域。

公开(公告)号：US6072624A

公开(公告)日：2000-06-06

申请号：US085445

申请日：1998-05-28

申请人： Arthur E. Dixon ,  Savvas Damaskinos

发明人： Arthur E. Dixon ,  Savvas Damaskinos

IPC分类号： G02B21/00 ,  G02B26/10 ,  G02B21/06

CPC分类号： G02B21/002 ,  G02B21/0056 ,  G02B21/0068 ,  G02B21/0076 ,  G02B26/101

摘要： A non-confocal scanning beam optical imaging or mapping system for macroscopic samples is disclosed, in which an expanded laser beam passes through a scan system and a telecentric laser scan lens focuses the beam to a moving spot in the sample. Light reflected (or emitted) from the illuminated spot is collected by the laser scan lens, descanned by the scan system, and detected by a detector. Transmitted-light and other non-confocal versions are also disclosed. This new scanning beam laser imaging system performs a rapid scan of a large sample. Applications include fluorescent imaging of large biomedical specimens, including fluorescent gels, photoluminescence mapping of semiconductor samples, etc.

摘要翻译： 公开了用于宏观样本的非共焦扫描光束成像或映射系统，其中扩展的激光束通过扫描系统，远心激光扫描透镜将光束聚焦到样品中的移动光点。 由照射光点反射（或发射）的光由扫描系统扫描并由检测器检测的激光扫描透镜收集。 还公开了透射光和其它非共焦型。 这种新的扫描光束激光成像系统对大样品进行快速扫描。 应用包括大型生物医学标本的荧光成像，包括荧光凝胶，半导体样品的光致发光映射等。

公开(公告)号：US5532873A

公开(公告)日：1996-07-02

申请号：US117797

申请日：1993-09-08

申请人： Arthur E. Dixon

发明人： Arthur E. Dixon

IPC分类号： G01B11/24 ,  G02B21/00 ,  G02B21/06

CPC分类号： G02B21/0048 ,  G02B21/0044 ,  G02B21/0068 ,  G02B21/0084

摘要： A new confocal scanning beam laser microscope or imaging system is disclosed which has a very wide range of magnification, which allows a small area of a specimen to be imaged at submicron resolution at high magnification, and a large area of the specimen, up to several centimeters in size, at lower resolution. The optical arrangement of a confocal scanning beam laser microscope is used to produce high resolution images of a small area of the specimen, using a microscope objective that is not telecentric. A telecentric laser scan lens is interchanged with the optical subassembly of the microscope comprised of the microscope objective and unitary telescope above it, such that the scanning beam now passes through the telecentric laser scan lens to produce lower resolution images of a much larger area of the specimen, and the imaging system acts as a confocal scanning beam macroscope. Several different embodiments are disclosed, with contrast mechanisms that include reflected light, fluorescence, photoluminescence, and optical beam induced current. A method for parfocalizing a microscope objective and a laser scan lens for use in the imaging system is also disclosed.

摘要翻译： 公开了一种新的共聚焦扫描光束激光显微镜或成像系统，其具有非常宽的放大倍率，这允许在高倍率下以亚微米分辨率和大面积的样品成像样本的小面积，直到几个 尺寸为厘米，分辨率较低。 使用共聚焦扫描光束激光显微镜的光学布置，使用不是远心的显微镜物镜来产生小面积的样品的高分辨率图像。 远心激光扫描透镜与由其上的显微镜物镜和整体望远镜组成的显微镜的光学子组件互换，使得扫描光束现在通过远心激光扫描透镜，以产生较大分辨率的图像 标本，成像系统作为共焦扫描光束宏观镜。 公开了几种不同的实施例，其中包括反射光，荧光，光致发光和光束感应电流的对比度机制。 还公开了一种用于使成像系统中使用的显微镜​​物镜和激光扫描透镜进行平焦的方法。

公开(公告)号：US5475728A

公开(公告)日：1995-12-12

申请号：US100274

申请日：1993-08-02

申请人： James A. Smith ,  Miroslav J. Stehlik ,  Matthew R. Atkinson ,  John H. Cole ,  Arthur E. Dixon ,  James H. Fierling ,  John Hennessy ,  Christopher J. L. Moore

发明人： James A. Smith ,  Miroslav J. Stehlik ,  Matthew R. Atkinson ,  John H. Cole ,  Arthur E. Dixon ,  James H. Fierling ,  John Hennessy ,  Christopher J. L. Moore

IPC分类号： G01N23/20

CPC分类号： G01N23/20

摘要： A motion system or goniometer for moving a sample supported by the system has a triangular plate supported at each apex by actuators that can extend or retract axially independently from one another. The actuators are mounted on x-y translation stages and a .theta. rotation stage. The movement of the actuators, translation stages and rotation stage is controlled by a computer. When it is desired to create a rocking curve at a particular position on the sample, the translation stages and rotation stage can remain stationary while moving only the plate and actuators. The goniometer is much more efficient and less expensive than previous devices which require movement of the entire goniometer when creating a rocking curve.

摘要翻译： 用于移动由系统支撑的样品的运动系统或测角器具有通过致动器支撑在每个顶点处的三角形板，其可以彼此轴向地轴向地伸展或缩回。 执行器安装在x-y平移台和θ旋转台上。 执行器，平移台和旋转台的运动由计算机控制。 当需要在样品上的特定位置创建摇摆曲线时，平移台和旋转台可以在仅移动板和致动器的同时保持静止。 测角仪比以前的设备更有效率和更便宜，这些设备在创建摇摆曲线时需要整个测角器的移动。

公开(公告)号：US08655043B2

公开(公告)日：2014-02-18

申请号：US12993010

申请日：2009-05-15

申请人： Arthur E. Dixon

发明人： Arthur E. Dixon

IPC分类号： G06K9/00

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G02B21/0024 ,  G02B21/16 ,  G02B21/367

摘要： A method of operating an instrument that is a macroscope, microscope, or slide scanner is provided where the instrument has a larger dynamic range for measurement than a dynamic range required in the final image of a specimen. In the method, data is measured from a specimen using the instrument, and the dynamic range of the measured data is contracted in the final image file during scanning.

摘要翻译： 提供操作作为宏观显微镜，显微镜或幻灯片扫描器的仪器的方法，其中仪器具有比测试的最终图像中所需的动态范围更大的测量动态范围。 在该方法中，使用仪器从样本测量数据，并且在扫描期间将测量数据的动态范围收缩在最终图像文件中。

公开(公告)号：US07218446B2

公开(公告)日：2007-05-15

申请号：US10648450

申请日：2003-08-27

申请人： Arthur E. Dixon ,  Savvas Damaskinos ,  Brian Wilson

发明人： Arthur E. Dixon ,  Savvas Damaskinos ,  Brian Wilson

IPC分类号： G02B21/00

CPC分类号： G02B21/0024 ,  G02B26/101

摘要： A new high resolution confocal and non-confocal scanning laser macroscope is disclosed which achieves fine focus and control of focus position by moving a lens in the intermediate optics. This arrangement is particularly useful for imaging specimens where it is difficult to focus by changing the distance between the scan lens and the specimen, for example for in-vivo imaging, photodynamic therapy, and image-guided surgery. It is also important to keep the lens-to-specimen distance constant when a liquid-immersion scan lens is used, in order to maintain a constant thickness of liquid between the lens and the specimen. In addition to being useful for confocal slicing, motion of the intermediate lens under computer control also enables dynamic focus and the ability to move the focal spot along a general path inside the specimen. Several applications of the imaging system are described. The macroscope images macroscopic specimens in reflected light, transmitted light, fluorescence, photoluminescence and multi-photon fluorescence.

摘要翻译： 公开了一种新的高分辨率共焦和非共焦扫描激光宏观显示器，其通过在中间光学器件中移动透镜来实现聚焦位置的精细对焦和控制。 这种布置对于通过改变扫描透镜和样本之间的距离难以聚焦的成像样品特别有用，例如用于体内成像，光动力学治疗和图像引导手术。 当使用液浸式扫描透镜时，保持透镜与样本之间的距离恒定也是重要的，以便在透镜和样本之间保持恒定的液体厚度。 除了用于共聚焦切片之外，中间透镜在计算机控制下的运动还能够实现动态聚焦和沿着标本内的通用路径移动焦点的能力。 描述成像系统的几个应用。 宏观图像宏观样本在反射光，透射光，荧光，光致发光和多光子荧光。

公开(公告)号：US5737121A

公开(公告)日：1998-04-07

申请号：US674145

申请日：1996-07-01

申请人： Arthur E. Dixon

发明人： Arthur E. Dixon

IPC分类号： G01B11/24 ,  G02B21/00 ,  G02B21/06

CPC分类号： G02B21/0048 ,  G02B21/0044 ,  G02B21/0068 ,  G02B21/0084

摘要： A new real-time confocal scanning laser macroscope is disclosed which images macroscopic specimens in reflected light and fluorescence or photoluminescence. The optical arrangement of a Nipkow disk microscope has been altered to include a telecentric scan lens which enables a large area of a specimen to be imaged with micron resolution, and when interchanged with a microscope objective, allows a small area of the specimen to be imaged with submicron resolution.

摘要翻译： 公开了一种新的实时共聚焦扫描激光宏观镜，其在反射光和荧光或光致发光中对宏观标本进行图像显影。 Nipkow圆盘显微镜的光学布置已经改变为包括远心扫描透镜，其使得能够以微米分辨率成像大面积的样本，并且当与显微镜物镜互换时，允许将样本的小面积成像 具有亚微米分辨率。

公开(公告)号：US5192980A

公开(公告)日：1993-03-09

申请号：US721282

申请日：1991-06-26

申请人： Arthur E. Dixon ,  Savvas Damaskinos ,  John W. Bowron

发明人： Arthur E. Dixon ,  Savvas Damaskinos ,  John W. Bowron

IPC分类号： G01J3/28 ,  G01J3/30 ,  G02B21/00

CPC分类号： G02B21/0072 ,  G01J3/30 ,  G02B21/0076 ,  G01J3/2889 ,  G01N21/6489

摘要： A scanning optical microscope or mapping system for spectrally-resolved measurement of light reflected, emitted or scatttered from a specimen is disclosed, in which the spectrally-resolving element is integrated into the detection arm of the microscope or mapping system to result in good photon collection efficiency as well as good spectral and spatial resolution. A confocal version of the microscope is disclosed which will be of particular interest in fluorescence microscopy, and the non-confocal mapping system will be of particular interest in photoluminescence mapping of semiconductor wafers.

摘要翻译： 公开了一种用于光谱分辨测量从样本反射，发射或刻蚀的光的扫描光学显微镜或测绘系统，其中光谱分辨元件被集成到显微镜或测绘系统的检测臂中以产生良好的光子收集 效率以及良好的光谱和空间分辨率。 公开了共聚焦版本的显微镜，其将在荧光显微镜中特别感兴趣，并且非共焦映射系统将在半导体晶片的光致发光映射中特别感兴趣。