公开(公告)号：US20210062156A1

公开(公告)日：2021-03-04

申请号：US17051528

申请日：2019-04-30

Applicant: Amgen Inc.

Inventor： Christopher Kenyon CROWELL ,  Jian W U ,  Athena Denise NAGI ,  Neil KITCHEN ,  Alison Jean GILLESPIE ,  Simina Crina PETROVAN ,  Michael Charles BRANDENSTEIN

IPC: C12N5/071 ,  C07K16/28

Abstract: This invention relates to recombinantly-expressed denosumab molecules and methods for modulating glycan profiles of denosumab molecules.

公开(公告)号：US20240384238A1

公开(公告)日：2024-11-21

申请号：US18779687

申请日：2024-07-22

Applicant: Amgen Inc.

Inventor： Christopher Kenyon CROWELL ,  Jian WU ,  Neil KITCHEN ,  Alison Jean GILLESPIE ,  Simina Crina PETROVAN ,  Michael Charles BRANDENSTEIN ,  Athena Denise Nagi

IPC: C12N5/071 ,  C07K16/28

Abstract: This invention relates to recombinantly-expressed denosumab molecules and methods for modulating glycan profiles of denosumab molecules.

公开(公告)号：US20170355741A1

公开(公告)日：2017-12-14

申请号：US15683521

申请日：2017-08-22

Applicant: AMGEN INC.

Inventor： Christopher Kenyon CROWELL ,  Gustavo Enrique GRAMPP

IPC: C07K14/505 ,  C12P21/00 ,  C07K14/575 ,  C12N5/00

CPC classification number: C07K14/505 ,  C07K14/575 ,  C12N5/0018 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2510/02 ,  C12P21/005

Abstract: Culture media comprising manganese and methods of culturing cells to improve sialylation and glycosylation of glycoproteins are provided.