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    CANCER MARKERS AND METHODS OF USE THEREOF
    3.
    发明申请
    CANCER MARKERS AND METHODS OF USE THEREOF 审中-公开
    癌症标记及其使用方法

    公开(公告)号：US20160274121A1

    公开(公告)日：2016-09-22

    申请号：US15005930

    申请日：2016-01-25

    申请人： Academia Sinica

    发明人： Chi-Huey Wong ,  Chung-Yi Wu ,  Sarah K.C. Cheung ,  Po-Kai Chuang ,  Tsui-Ling Hsu

    IPC分类号： G01N33/574 ,  C07K16/30

    CPC分类号： G01N33/57492 ,  A61K2039/505 ,  C07K16/18 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/734 ,  C07K2317/92 ,  G01N33/5073 ,  G01N33/57407 ,  G01N2333/70585 ,  G01N2333/70596 ,  G01N2405/10 ,  G01N2500/04 ,  G01N2500/10 ,  G01N2800/52

    摘要： The present disclosure relates to methods and compositions which can modulate the globoseries glycosphingolipid synthesis. Particularly, the present disclosure is directed to glycoenzyme inhibitor compound and compositions and methods of use thereof that can modulate the synthesis of globoseries glycosphingolipid SSEA-3/SSEA-4/GloboH in the biosynthetic pathway; particularly, the glycoenzyme inhibitors target the alpha-4GalT; beta-4GalNAcT-I; or beta-3GalT-V enzymes in the globoseries synthetic pathway. Additionally, the present disclosure is also directed to vaccines, antibodies, and/or immunogenic conjugate compositions targeting the SSEA-3/SSEA-4/GLOBO H associated epitopes (natural and modified) which elicit antibodies and/or binding fragment production useful for modulating the globoseries glycosphingolipid synthesis. Moreover, the present disclosure is also directed to the method of using the compositions described herein for the treatment or detection of hyperproliferative diseases and/or conditions. Furthermore, the instant disclosure also relates to cancer stem cell biomarkers for disgnostic and therapeutic uses.

    摘要翻译： 本公开涉及可以调节球形蛋白糖鞘脂合成的方法和组合物。 特别地，本公开涉及可以调节生物合成途径中的球形蛋白鞘糖脂SSEA-3 / SSEA-4 / GloboH的合成的糖酵母抑制剂化合物及其组合物和使用方法; 特别是糖酵解抑制剂靶向α-4GalT; β-4GalNAcT-I; 或β-3GalT-V酶在球形蛋白合成途径中。 另外，本公开还涉及靶向SSEA-3 / SSEA-4 / GLOBO H相关表位（天然和修饰）的疫苗，抗体和/或免疫原性缀合物组合物，其引发可用于调节的抗体和/或结合片段产生 全球糖苷脂蛋白合成。 此外，本公开还涉及使用本文所述的组合物治疗或检测过度增殖性疾病和/或病症的方法。 此外，本公开还涉及用于预后和治疗用途的癌症干细胞生物标志物。

    Alkynyl sugar analogs for labeling and visualization of glycoconjugates in cells
    5.
    发明授权
    Alkynyl sugar analogs for labeling and visualization of glycoconjugates in cells 有权

    公开(公告)号：US10317393B2

    公开(公告)日：2019-06-11

    申请号：US15729317

    申请日：2017-10-10

    申请人： Academia Sinica

    发明人： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Sarah R. Hanson ,  Masaaki Sawa

    IPC分类号： G01N33/50 ,  G01N33/533

    摘要： Methods for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates are disclosed. Alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars are incorporated into cellular glycoconjugates. Chemical probes comprising an azide group and a visual or fluorogenic probe and used to label alkyne-derivatized sugar-tagged glycoconjugates are disclosed. Chemical probes bind covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and are visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA, confocal microscopy, and mass spectrometry.