公开(公告)号：US11952575B2

公开(公告)日：2024-04-09

申请号：US16652291

申请日：2017-11-15

Applicant: Asymchem Life Science (Tianjin) Co., Ltd

Inventor： Hao Hong ,  Gage James ,  Jiangping Lu ,  Xingfu Xu ,  Wenyan Yu ,  Xin Huang ,  Yulei Ma ,  Yibing Cheng

IPC: C12N9/00 ,  C12N15/70 ,  C12P13/00

CPC classification number: C12N15/70 ,  C12P13/001 ,  C12Y206/01

Abstract: A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.

公开(公告)号：US12209258B2

公开(公告)日：2025-01-28

申请号：US17434436

申请日：2019-04-17

Applicant: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

Inventor： Hao Hong ,  Gage James ,  Jiangping Lu ,  Na Zhang ,  Yulei Ma ,  Yibing Cheng ,  Huiyan Mu

IPC: C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12N15/70 ,  C12N15/81 ,  C12P7/26 ,  C12P17/18

Abstract: Disclosed are a monooxygenase mutant and use thereof. An amino acid sequence of a monooxygenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO: 1. Mutation includes at least one of the following mutation sites: site 49, site 60, site 61, site 144, site 145, site 146, site 147, site 167, site 169, site 189, site 246, site 247, site 280, site 284, site 285, site 286, site 287, site 328, site 330, site 332, site 382, site 427, site 428, site 429, site 430, site 431, site 432, site 433, site 434, site 435, site 436, site 438, site 441, site 493, site 494, site 508, site 509, site 510, site 511, site 512, and site 513 sites and the like.

公开(公告)号：US11359219B2

公开(公告)日：2022-06-14

申请号：US16966528

申请日：2018-02-05

Applicant: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD

Inventor： Hao Hong ,  Gage James ,  Jiangping Lu ,  Xingfu Xu ,  Yuxia Cui ,  Na Zhang ,  Xuewu Dong ,  Wenyan Yu ,  Xin Huang ,  Mingmin Hao ,  Yulei Ma ,  Yibing Cheng ,  Jiadong Zhao

IPC: C12P17/12 ,  C12P17/10 ,  C12N9/10 ,  C12N11/082 ,  C12N11/00 ,  C12N11/10

Abstract: The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.

公开(公告)号：US20220145341A1

公开(公告)日：2022-05-12

申请号：US17434436

申请日：2019-04-17

Applicant: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

Inventor： Hao Hong ,  Gage James ,  Jiangping Lu ,  Na Zhang ,  Yulei Ma ,  Yibing Cheng ,  Huiyan Mu

IPC: C12P17/18 ,  C12N9/02 ,  C12N15/70 ,  C12N15/81

Abstract: Disclosed are a monooxygenase mutant and use thereof. An amino acid sequence of a monooxygenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO: 1. Mutation includes at least one of the following mutation sites: site 49, site 60, site 61, site 144, site 145, site 146, site 147, site 167, site 169, site 189, site 246, site 247, site 280, site 284, site 285, site 286, site 287, site 328, site 330, site 332, site 382, site 427, site 428, site 429, site 430, site 431, site 432, site 433, site 434, site 435, site 436, site 438, site 441, site 493, site 494, site 508, site 509, site 510, site 511, site 512, and site 513 sites and the like. The monooxygenase mutant has the advantage of greatly improved stereoselectivity, and the enzyme activity is also improved correspondingly.