公开(公告)号：US20230287335A1

公开(公告)日：2023-09-14

申请号：US18018389

申请日：2021-07-27

Applicant: AMGEN INC.

Inventor： Nicholas MARCHAND ,  Glen BOLTON ,  Sara Ann GALL ,  Jian WU

IPC: C12N5/00 ,  B01D61/02 ,  B01D65/08

CPC classification number: C12N5/0018 ,  B01D61/027 ,  B01D65/08 ,  C12N2501/105 ,  C12N2501/33 ,  B01D2321/168

Abstract: Presented herein are methods for preparing and treating mammalian cell culture media through the addition of low levels of poloxamer and nanofiltration. Low levels of poloxamer improved flux and retained greater levels of a growth factor.

公开(公告)号：US20240279704A1

公开(公告)日：2024-08-22

申请号：US18567747

申请日：2022-06-06

Applicant: AMGEN INC.

Inventor： Namratha SUBHASH ,  Yu Cong CHEN ,  Kyle Shamus MCELEARNEY ,  Glen BOLTON

IPC: C12P21/00 ,  C07K1/22 ,  C07K1/34 ,  C07K16/24 ,  C12N9/24

CPC classification number: C12P21/005 ,  C07K1/22 ,  C07K1/34 ,  C07K16/244 ,  C12N9/2402 ,  C07K2317/14 ,  C07K2317/41 ,  C07K2317/72 ,  C12Y302/01051

Abstract: Provided herein are methods of obtaining a recombinant glycosylated protein having increased levels of afucosylated glycoforms. In exemplary embodiments, the methods comprise incubating purified recombinant glycosylated protein with a human broad specificity fucosidase and separating the recombinant glycosylated protein from the fucosidase.