公开(公告)号：US20240151714A1

公开(公告)日：2024-05-09

申请号：US18281460

申请日：2022-03-14

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Tae Hyeon YOO ,  Hyeon Ji PARK

IPC: G01N33/53 ,  G01N33/573 ,  G01N33/68 ,  G01N33/74 ,  G01N33/76

CPC classification number: G01N33/5308 ,  G01N33/573 ,  G01N33/6887 ,  G01N33/74 ,  G01N33/76 ,  G01N2333/4712 ,  G01N2333/59 ,  G01N2333/71 ,  G01N2333/96463

Abstract: The present invention relates to a target analyte detection composition based on a proximity proteolysis reaction and a target analyte detection method using same. More specifically, the present invention relates to a method for detecting a target analyte, the method comprising a step in which when a first binder and a second binder bind to the target analyte, hybridization is made between ssDNA linked to the first binder and ssDNA linked to a protease and between ssDNA linked to the second binder and ssDNA linked to a zymogen, whereby a signal generated by a proximity proteolysis reaction between the protease and the zymogen is detected.

公开(公告)号：US20210348219A1

公开(公告)日：2021-11-11

申请号：US17282650

申请日：2019-10-02

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Tae Hyeon YOO ,  Hyeon Ji PARK

IPC: C12Q1/6834 ,  G01N33/53 ,  C12Q1/6818 ,  G01N21/64 ,  G01N21/31

Abstract: A method for detecting a target nucleic acid includes: (a) a step of mixing a sample containing the target nucleic acid with a nucleic acid detection solution containing i) ssDNA-protease conjugate, ii) ssDNA-zymogen conjugate, and iii) a substrate specific for the zymogen; and (b) a step of detecting a signal generated by a proximity proteolysis reaction between the ssDNA-zymogen conjugate and the ssDNA-protease conjugate which are hybridized to the target nucleic acid.