公开(公告)号：US20200030486A1

公开(公告)日：2020-01-30

申请号：US16338140

申请日：2017-10-03

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Jonathan J. O'HARE ,  Mikhail L. PEKUROVSKY ,  Amy S. DETERMAN ,  Gino L. PITERA ,  Jason W. BJORK ,  Daniel V. NORTON ,  Robert A. ASMUS

IPC: A61L26/00 ,  B29C43/24

Abstract: A method of forming a fibrin hydrogel composition including providing one or more unitary masses of a fibrin hydrogel, dividing at least one of the unitary masses of the fibrin hydrogel into a multiplicity of smaller pieces of the fibrin hydrogel, and recombining at least a portion of the smaller pieces into a cohesive mass. Dividing at least one of the unitary masses of fibrin hydrogel into a multiplicity of smaller pieces may include shearing or cutting the unitary masses to form an aqueous dispersion of the fibrin hydrogel in an aqueous medium. The aqueous dispersion of fibrin hydrogel may be applied to a substrate on a roller or an endless belt, and is optionally overlaid by a scrim. The cohesive mass of fibrin hydrogel, which may be formed by removing at least a portion of the aqueous medium from the aqueous dispersion of the smaller pieces of the fibrin hydrogel, finds uses in wound dressing articles.

公开(公告)号：US20160257921A1

公开(公告)日：2016-09-08

申请号：US15030391

申请日：2014-10-20

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Jason W. BJORK ,  Mara S. CELT ,  Adam J. STANENAS

IPC: C12M1/34 ,  C12M1/00 ,  C12Q1/04 ,  C12Q1/26

CPC classification number: C12M41/36 ,  C12M23/20 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/26 ,  G01N2333/90225 ,  G01N2333/90235

Abstract: A self-contained anaerobic environment-generating culture device is provided. The culture device includes a first substrate having opposing inner and outer surfaces, a second substrate having opposing inner and outer surfaces, a growth region disposed between the inner surfaces of the first and second substrates, an effective amount of a substantially dry enzyme component of an enzyme-mediated oxygen depletion system, an effective amount of a substantially dry enzyme substrate component of the enzyme-mediated oxygen depletion system, and a dry, cold-water-soluble gelling agent disposed in the growth region. The enzyme and enzyme substrate components are disposed in coatings in the growth region. The first and second substrates are substantially nontransmissible to gaseous oxygen. Methods of making and using the culture device area also provided.

Abstract translation: 提供了一种独立的厌氧环境培养装置。 培养装置包括具有相对的内表面和外表面的第一基底，具有相对的内表面和外表面的第二基底，设置在第一和第二基底的内表面之间的生长区域，有效量的基本上干燥的酶组分 酶介导的氧耗尽系统，有效量的酶介导的氧耗尽系统的基本干燥的酶底物组分，以及设置在生长区域中的干燥的冷水溶性胶凝剂。 将酶和酶底物组分置于生长区的涂层中。 第一和第二基底基本上不能透过气态氧气。 还提供了制作和使用培养装置领域的方法。