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公开(公告)号：US20210002721A1
公开(公告)日：2021-01-07
申请号：US16844141
申请日：2020-04-09
申请人： 10x Genomics, Inc.
IPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US20190382836A1
公开(公告)日：2019-12-19
申请号：US16395090
申请日：2019-04-25
申请人： 10X GENOMICS, INC.
IPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US20190203262A1
公开(公告)日：2019-07-04
申请号：US16242962
申请日：2019-01-08
申请人： 10X Genomics, Inc.
发明人： Benjamin Hindson , Christopher Hindson , Michael Schnall-Levin , Kevin Ness , Mirna Jarosz , Donald Masquelier , Serge Saxonov , Landon Merrill , Andrew Price , Paul Hardenbol , Yuan Li
IPC分类号： C12Q1/6806 , C12N15/10
CPC分类号： C12Q1/6806 , B01J2219/005 , B01J2219/00547 , B01J2219/00572 , B01J2219/00722 , C12N15/1006 , C12N15/1065 , C12Q2535/122 , C12Q2563/159
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US10323279B2
公开(公告)日：2019-06-18
申请号：US16052486
申请日：2018-08-01
申请人： 10X GENOMICS, INC.
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US20240002929A1
公开(公告)日：2024-01-04
申请号：US18186088
申请日：2023-03-17
申请人： 10x Genomics, Inc.
IPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q1/6855 , C12Q1/6806
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US20210180044A1
公开(公告)日：2021-06-17
申请号：US17093050
申请日：2020-11-09
申请人： 10X GENOMICS, INC.
IPC分类号： C12N15/10
摘要： The present disclosure provides methods and systems for generation of compositions comprising two or more sets of molecules. Compositions herein may comprise sets of molecules of different types. Sets of molecules may be generated by attachment to supports. Different types of molecules may be used to generate a large number of unique molecules. One or more types of molecules may be used to identify one or more additional types of molecules. Compositions of the present disclosure may be used in, for example, nucleic acid sequencing.
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公开(公告)号：US20190316197A1
公开(公告)日：2019-10-17
申请号：US16435362
申请日：2019-06-07
申请人： 10X GENOMICS, INC.
IPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US12049621B2
公开(公告)日：2024-07-30
申请号：US17093050
申请日：2020-11-09
申请人： 10X GENOMICS, INC.
IPC分类号： C12N15/10
CPC分类号： C12N15/1065
摘要： The present disclosure provides methods and systems for generation of compositions comprising two or more sets of molecules. Compositions herein may comprise sets of molecules of different types. Sets of molecules may be generated by attachment to supports. Different types of molecules may be used to generate a large number of unique molecules. One or more types of molecules may be used to identify one or more additional types of molecules. Compositions of the present disclosure may be used in, for example, nucleic acid sequencing.
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公开(公告)号：US20180363029A1
公开(公告)日：2018-12-20
申请号：US16056231
申请日：2018-08-06
申请人： 10X Genomics, Inc.
发明人： Benjamin Hindson , Christopher Hindson , Michael Schnall-Levin , Kevin Ness , Mirna Jarosz , Donald Masquelier , Serger Saxonov , Landon Merrill , Andrew Price , Paul Hardenbol , Yuan Li
IPC分类号： C12Q1/6806 , C12N15/10
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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公开(公告)号：US20180094298A1
公开(公告)日：2018-04-05
申请号：US15718764
申请日：2017-09-28
申请人： 10X Genomics, Inc.
发明人： Benjamin Hindson , Christopher Hindson , Michael Schnall-Levin , Kevin Ness , Mirna Jarosz , Donald Masquelier , Serge Saxonov , Landon Merrill , Andrew Price , Paul Hardenbol , Yuan Li
CPC分类号： C12Q1/6806 , B01J2219/005 , B01J2219/00547 , B01J2219/00572 , B01J2219/00722 , C12N15/1006 , C12N15/1065 , C12Q2535/122 , C12Q2563/159
摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
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