公开(公告)号：US20240002929A1

公开(公告)日：2024-01-04

申请号：US18186088

申请日：2023-03-17

申请人： 10x Genomics, Inc.

发明人： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20220340968A1

公开(公告)日：2022-10-27

申请号：US17860880

申请日：2022-07-08

申请人： 10x Genomics, Inc.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11021749B2

公开(公告)日：2021-06-01

申请号：US16998414

申请日：2020-08-20

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Kevin Ness ,  Serge Saxonov ,  Paul Hardenbol

IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10533221B2

公开(公告)日：2020-01-14

申请号：US16165389

申请日：2018-10-19

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/683 ,  C12N15/10 ,  C12N15/00 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

公开(公告)号：US20190264278A9

公开(公告)日：2019-08-29

申请号：US16212441

申请日：2018-12-06

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6855

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10253364B2

公开(公告)日：2019-04-09

申请号：US15980473

申请日：2018-05-15

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mima Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190024166A1

公开(公告)日：2019-01-24

申请号：US16138448

申请日：2018-09-21

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

CPC分类号： C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190017115A9

公开(公告)日：2019-01-17

申请号：US15980473

申请日：2018-05-15

申请人： 10X GENOMICS, INC.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mima Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20170114390A1

公开(公告)日：2017-04-27

申请号：US15376582

申请日：2016-12-12

申请人： 10X Genomics, Inc.

发明人： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Mirna Jarosz

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20210002721A1

公开(公告)日：2021-01-07

申请号：US16844141

申请日：2020-04-09

申请人： 10x Genomics, Inc.

发明人： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.