High throughput in vitro translation (cell-lysate based) assay for detecting quorum sensing signals
    1.
    发明授权
    High throughput in vitro translation (cell-lysate based) assay for detecting quorum sensing signals 有权
    用于检测群体感应信号的高通量体外翻译(基于细胞裂解物)测定

    公开(公告)号:US08932807B2

    公开(公告)日:2015-01-13

    申请号:US14043052

    申请日:2013-10-01

    摘要: A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., Agrobacterium tumefaciens NTL4 (pCF218)(pCF372)) and a detecting substrate (e.g., an absorbance-based or luminescence-based substrate). The cell-lysate extract can be prepared by (1) disrupting the cell membranes of the biosensor bacterium to release the cellular components into a solution, (2) centrifuging the resulting solution, and (3) removing the resulting supernatant solution.

    摘要翻译: 通常提供用于检测群体感测信号的基于细胞裂解液提取物的测定试剂及其制备和使用方法。 测定试剂通常包括由生物传感器细菌(例如根癌土壤杆菌NTL4(pCF218)(pCF372))和检测底物(例如基于吸光度或发光的基底)形成的细胞裂解物提取物。 细胞裂解物提取物可以通过(1)破坏生物传感器细菌的细胞膜以将细胞成分释放到溶液中来制备,(2)离心所得溶液,和(3)除去所得上清液。

    Rosin-Derived Cationic Compounds and Polymers
    3.
    发明申请
    Rosin-Derived Cationic Compounds and Polymers 有权
    松香衍生的阳离子化合物和聚合物

    公开(公告)号:US20140163174A1

    公开(公告)日:2014-06-12

    申请号:US14101630

    申请日:2013-12-10

    摘要: Methods for forming rosin-derived cationic compounds are provided. The method can include attaching a cationic group to a conjugated diene on a hydrophenathrene-based ring of a resin acid (e.g., levopimaric acid, abietic acid, dehydroabietic acid, or a mixture thereof) to form a rosin-derived cationic compound. Attaching the cationic group to the conjugated diene on the hydrophenathrene-based ring of the resin acid can be achieved via a Diels-Alder reaction of a dienophile with the hydrophenathrene-based ring of the resin acid. Rosin-derived cationic compounds are also provided. The rosin-derived cationic compound can include a cationic group attached to a conjugated diene on a hydrophenathrene-based ring of a resin acid, wherein the rosin-derived cationic compound further comprises a carboxylic acid group.

    摘要翻译: 提供了形成松香衍生的阳离子化合物的方法。 该方法可以包括将阳离子基团连接到树脂酸(例如,左旋海松酸,松果酸,脱氢松香酸或其混合物)的基于氢化苯环基的环上的共轭二烯上,以形成松香衍生的阳离子化合物。 将阳离子基团连接到树脂酸的氢化苯环类环上的共轭二烯上可以通过亲二烯体与树脂酸的氢化苯基萘基环的Diels-Alder反应来实现。 还提供了松香衍生的阳离子化合物。 松香衍生的阳离子化合物可以包括在树脂酸的基于氢化苯环的环上连接到共轭二烯上的阳离子基团,其中所述松香衍生的阳离子化合物还包含羧酸基团。

    High-throughput in vitro translation (cell-lysate based) assay for detecting quorum sensing signals
    4.
    发明授权
    High-throughput in vitro translation (cell-lysate based) assay for detecting quorum sensing signals 有权
    用于检测群体感应信号的高通量体外翻译(基于细胞裂解物)测定

    公开(公告)号:US08546071B2

    公开(公告)日:2013-10-01

    申请号:US12180857

    申请日:2008-07-28

    IPC分类号: C12Q1/00

    摘要: A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., Agrobacterium tumefaciens NTL4 (pCF218)(pCF372)) and a detecting substrate (e.g., an absorbance-based or luminescence-based substrate). The cell-lysate extract can be prepared by (1) disrupting the cell membranes of the biosensor bacterium to release the cellular components into a solution, (2) centrifuging the resulting solution, and (3) removing the resulting supernatant solution.

    摘要翻译: 通常提供用于检测群体感测信号的基于细胞裂解液提取物的测定试剂及其制备和使用方法。 测定试剂通常包括由生物传感器细菌(例如根癌土壤杆菌NTL4(pCF218)(pCF372))和检测底物(例如基于吸光度或发光的基底)形成的细胞裂解物提取物。 细胞裂解物提取物可以通过(1)破坏生物传感器细菌的细胞膜以将细胞成分释放到溶液中来制备,(2)离心所得溶液,和(3)除去所得上清液。

    Method of micro/nano imprinting
    5.
    发明申请
    Method of micro/nano imprinting 有权
    微/纳刻印方法

    公开(公告)号:US20090194913A1

    公开(公告)日:2009-08-06

    申请号:US12230660

    申请日:2008-09-03

    IPC分类号: B29C59/12

    摘要: Disclosed is a method of micro/nano imprinting, which applies soft mold, pre-shaping sealing film, and soft holder arrangements to the micro/nano structure imprinting process of the curved substrates. The method of the present invention can prevent the curved surface from crumbling, which may result from high gas pressuring, and can obtain uniform imprinting pressure distribution throughout the whole curved substrate. Moreover, replicating micro/nano structures onto double-sided curve, both convex and concave, surfaces can also be achieved.

    摘要翻译: 公开了一种微/纳刻印方法,其将柔性模具,预成型密封膜和软夹具布置应用于弯曲基板的微/纳结构压印过程。 本发明的方法可以防止由于气体压力高而导致的曲面破碎,并且可以在整个弯曲基板上获得均匀的压印压力分布。 此外,还可以实现将微/纳米结构复制到双面曲线上,凸表面和凹面两者。

    Method for decomposing toxic organic pollutants
    6.
    发明授权
    Method for decomposing toxic organic pollutants 有权
    分解有毒有机污染物的方法

    公开(公告)号:US06087547A

    公开(公告)日:2000-07-11

    申请号:US318593

    申请日:1999-05-25

    IPC分类号: A62D3/02 A62D3/00 B09C1/10

    CPC分类号: B09C1/105 B09C1/10

    摘要: It has been discovered that an enzyme produced by Spartina alterniflora degrades halogenated organics. The present invention is therefore the use of the enzyme produced by genes of this plant to remediate soils contaminated with halogenated organics such as TCE, and to genetically alter other plants to produce this enzyme using the gene that produces plant dehaloperoxidase.

    摘要翻译: 已经发现由交替花粉生产的酶降解卤代有机物。 因此,本发明是使用由该植物的基因产生的酶来修复被卤代有机物如TCE污染的土壤,并且使用产生植物脱卤酶的基因遗传地改变其它植物以产生该酶。

    Rathayibacter biopuresis produces cephalosporin haloperoxidase
    7.
    发明授权
    Rathayibacter biopuresis produces cephalosporin haloperoxidase 失效
    Rathayibacter biopuresis产生头孢菌素卤过氧化物酶

    公开(公告)号:US5854054A

    公开(公告)日:1998-12-29

    申请号:US777541

    申请日:1996-12-30

    摘要: An enzyme preparation that exhibits cephalosporin haloperoxidase activity is isolatable from a microorganism species of the Rathayibacter genus. This enzyme preparation can convert cephalexin to a halogenated cephalosporin antibiotic in a single step. A particular, unique microorganism that can provide the cephalosporin haloperoxidase enzyme preparation is Rathayibacter biopuresis.

    摘要翻译: 显示头孢菌素卤代过氧化物酶活性的酶制剂可从Rathayibacter属的微生物物种分离。 该酶制剂可以在单一步骤中将头孢氨苄转化为卤代头孢菌素抗生素。 可以提供头孢菌素卤过氧化物酶制剂的特殊的独特微生物是生物利用生物利用物质。

    Rosin-Derived Cationic Compounds and Polymers
    8.
    发明申请
    Rosin-Derived Cationic Compounds and Polymers 审中-公开
    松香衍生的阳离子化合物和聚合物

    公开(公告)号:US20150073092A1

    公开(公告)日:2015-03-12

    申请号:US14542769

    申请日:2014-11-17

    摘要: Methods for forming rosin-derived cationic compounds are provided. The method can include attaching a cationic group to a conjugated diene on a hydrophenathrene-based ring of a resin acid (e.g., levopimaric acid, abietic acid, dehydroabietic acid, or a mixture thereof) to form a rosin-derived cationic compound. Attaching the cationic group to the conjugated diene on the hydrophenathrene-based ring of the resin acid can be achieved via a Diels-Alder reaction of a dienophile with the hydrophenathrene-based ring of the resin acid. Rosin-derived cationic compounds are also provided. The rosin-derived cationic compound can include a cationic group attached to a conjugated diene on a hydrophenathrene-based ring of a resin acid, wherein the rosin-derived cationic compound further comprises a carboxylic acid group.

    摘要翻译: 提供了形成松香衍生的阳离子化合物的方法。 该方法可以包括将阳离子基团连接到树脂酸(例如,左旋海松酸,松果酸,脱氢松香酸或其混合物)的基于氢化苯环基的环上的共轭二烯上,以形成松香衍生的阳离子化合物。 将阳离子基团连接到树脂酸的氢化苯环类环上的共轭二烯上可以通过亲二烯体与树脂酸的氢化苯基萘系环的Diels-Alder反应来实现。 还提供了松香衍生的阳离子化合物。 松香衍生的阳离子化合物可以包括在树脂酸的基于氢化苯环的环上连接到共轭二烯上的阳离子基团,其中所述松香衍生的阳离子化合物还包含羧酸基团。

    Rosin-derived cationic compounds and polymers
    9.
    发明授权
    Rosin-derived cationic compounds and polymers 有权
    松香衍生的阳离子化合物和聚合物

    公开(公告)号:US08901245B2

    公开(公告)日:2014-12-02

    申请号:US14101630

    申请日:2013-12-10

    摘要: Methods for forming rosin-derived cationic compounds are provided. The method can include attaching a cationic group to a conjugated diene on a hydrophenathrene-based ring of a resin acid (e.g., levopimaric acid, abietic acid, dehydroabietic acid, or a mixture thereof) to form a rosin-derived cationic compound. Attaching the cationic group to the conjugated diene on the hydrophenathrene-based ring of the resin acid can be achieved via a Diels-Alder reaction of a dienophile with the hydrophenathrene-based ring of the resin acid. Rosin-derived cationic compounds are also provided. The rosin-derived cationic compound can include a cationic group attached to a conjugated diene on a hydrophenathrene-based ring of a resin acid, wherein the rosin-derived cationic compound further comprises a carboxylic acid group.

    摘要翻译: 提供了形成松香衍生的阳离子化合物的方法。 该方法可以包括将阳离子基团连接到树脂酸(例如,左旋海松酸,松果酸,脱氢松香酸或其混合物)的基于氢化苯环基的环上的共轭二烯上,以形成松香衍生的阳离子化合物。 将阳离子基团连接到树脂酸的氢化苯环类环上的共轭二烯上可以通过亲二烯体与树脂酸的氢化苯基萘基环的Diels-Alder反应来实现。 还提供了松香衍生的阳离子化合物。 松香衍生的阳离子化合物可以包括在树脂酸的基于氢化苯环的环上连接到共轭二烯上的阳离子基团,其中所述松香衍生的阳离子化合物还包含羧酸基团。

    HIGH-THROUGHPUT IN VITRO TRANSLATION (CELL-LYSATE BASED) ASSAY FOR DETECTING QUORUM SENSING SIGNALS
    10.
    发明申请
    HIGH-THROUGHPUT IN VITRO TRANSLATION (CELL-LYSATE BASED) ASSAY FOR DETECTING QUORUM SENSING SIGNALS 有权
    用于检测相同感测信号的体外转录(基于细胞基)的高分辨率测定

    公开(公告)号:US20120164672A1

    公开(公告)日:2012-06-28

    申请号:US12180857

    申请日:2008-07-28

    IPC分类号: G01N21/76

    摘要: A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., Agrobacterium tumefaciens NTL4 (pCF218)(pCF372)) and a detecting substrate (e.g., an absorbance-based or luminescence-based substrate). The cell-lysate extract can be prepared by (1) disrupting the cell membranes of the biosensor bacterium to release the cellular components into a solution, (2) centrifuging the resulting solution, and (3) removing the resulting supernatant solution.

    摘要翻译: 通常提供用于检测群体感测信号的基于细胞裂解液提取物的测定试剂及其制备和使用方法。 测定试剂通常包括由生物传感器细菌(例如根癌土壤杆菌NTL4(pCF218)(pCF372))和检测底物(例如基于吸光度或发光的基底)形成的细胞裂解物提取物。 细胞裂解物提取物可以通过(1)破坏生物传感器细菌的细胞膜以将细胞成分释放到溶液中来制备,(2)离心所得溶液,和(3)除去所得上清液。