公开(公告)号：US08278630B1

公开(公告)日：2012-10-02

申请号：US13544716

申请日：2012-07-09

申请人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US08242463B2

公开(公告)日：2012-08-14

申请号：US13399820

申请日：2012-02-17

申请人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US08158926B2

公开(公告)日：2012-04-17

申请号：US13099062

申请日：2011-05-02

申请人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

发明人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

IPC分类号： H01L27/00

CPC分类号： G01N21/6456 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G02B21/0032 ,  G02B21/004

摘要： The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

摘要翻译： 本发明提供了用于以快速扫描速率获得样品的高分辨率图像的成像装置和方法。 具有比垂直尺寸长的水平尺寸的矩形检测器阵列可以与定位成将样本的一部分的矩形图像引导到矩形检测器阵列的成像光学元件一起使用。 扫描装置可以被配置成以扫描轴尺寸扫描样本，其中矩形检测器阵列的垂直尺寸和图像的两个矩形尺寸中的较短的尺寸在扫描轴尺寸上，并且其中垂直尺寸 因为矩形检测器阵列足够短以在单个轴上实现共焦。

公开(公告)号：US08085064B2

公开(公告)日：2011-12-27

申请号：US12823266

申请日：2010-06-25

申请人： Wenyi Feng ,  Jonathan Greene

发明人： Wenyi Feng ,  Jonathan Greene

IPC分类号： H03K19/173 ,  G06F7/38

CPC分类号： H03K19/1737 ,  G06F7/503 ,  H03K19/17728

摘要： A logic module for an FPGA includes a LUT formed from an N-level tree of 2:1 multiplexers. Each of the N inputs to the LUT is connected to the select inputs of the multiplexers in one level of the tree. Each of the data inputs at the leaves of the tree is driven by a configuration memory cell that produces either a logic 0 or a logic 1. The output of the single multiplexer at the last level of the tree forms a Y output and is coupled to one input of an XOR gate and to the select input of a two-input carry multiplexer. The 0 input of the carry multiplexer is coupled to a G input. A CI input is coupled to the other input of the XOR gate and to the 1 input of the carry multiplexer.

摘要翻译： 用于FPGA的逻辑模块包括由2：1多路复用器的N级树形成的LUT。 LUT的N个输入中的每一个连接到树的一个级别中的多路复用器的选择输入。 在树的叶片处的每个数据输入由产生逻辑0或逻辑1的配置存储器单元驱动。在树的最后一级的单个多路复用器的输出形成Y输出，并耦合到 XOR门的一个输入和双输入进位多路复用器的选择输入。 进位多路复用器的0输入耦合到G输入。 CI输入耦合到XOR门的另一个输入端和进位复用器的1个输入端。

公开(公告)号：US20110301044A1

公开(公告)日：2011-12-08

申请号：US13209306

申请日：2011-08-12

申请人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： C40B20/08 ,  C40B60/10 ,  C40B20/04

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US07960685B2

公开(公告)日：2011-06-14

申请号：US12537100

申请日：2009-08-06

申请人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

发明人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

IPC分类号： H01L27/00

CPC分类号： G01N21/6456 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G02B21/0032 ,  G02B21/004

摘要： The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

摘要翻译： 本发明提供了用于以快速扫描速率获得样品的高分辨率图像的成像装置和方法。 具有比垂直尺寸长的水平尺寸的矩形检测器阵列可以与定位成将样本的一部分的矩形图像引导到矩形检测器阵列的成像光学元件一起使用。 扫描装置可以被配置成以扫描轴尺寸扫描样本，其中矩形检测器阵列的垂直尺寸和图像的两个矩形尺寸中的较短的尺寸在扫描轴尺寸上，并且其中垂直尺寸 因为矩形检测器阵列足够短以在单个轴上实现共焦。

公开(公告)号：US20100259763A1

公开(公告)日：2010-10-14

申请号：US12822325

申请日：2010-06-24

申请人： Wenyi Feng

发明人： Wenyi Feng

IPC分类号： G01B11/02

CPC分类号： G02B21/006 ,  G01N2015/1452 ,  G02B21/008

摘要： Systems and methods for facilitating focusing of an image scanner, such as a confocal microscope, are disclosed. Measurement of optical characteristics in certain areas of a test sample are compared to stored or baseline optical characteristic profiles to determine an appropriate correction to properly focus the scanner. In one aspect, the method includes obtaining a dynamic profile at a current detection region of a test sample and associating the dynamic profile to a profile selected from a set of stored baseline profiles. Each of the stored baseline profiles is associated with a correction.

摘要翻译： 公开了用于促进诸如共焦显微镜的图像扫描仪的聚焦的系统和方法。 将测试样品的某些区域中的光学特性的测量与存储的或基线光学特性曲线进行比较，以确定适当的校正以适当地聚焦扫描仪。 在一个方面，该方法包括在测试样本的当前检测区域获得动态分布，并将动态分布与从一组存储的基线分布中选择的分布相关联。 每个存储的基准曲线与校正相关联。

公开(公告)号：US07772879B1

公开(公告)日：2010-08-10

申请号：US12101589

申请日：2008-04-11

申请人： Wenyi Feng ,  Jonathan Greene

发明人： Wenyi Feng ,  Jonathan Greene

IPC分类号： G06F7/38 ,  H03K19/173

CPC分类号： H03K19/1737 ,  G06F7/503 ,  H03K19/17728

摘要： A logic module for an FPGA includes a LUT formed from an N-level tree of 2:1 multiplexers. Each of the N inputs to the LUT is connected to the select inputs of the multiplexers in one level of the tree. Each of the data inputs at the leaves of the tree is driven by a configuration memory cell that produces either a logic 0 or a logic 1. The output of the single multiplexer at the last level of the tree forms a Y output and is coupled to one input of an XOR gate and to the select input of a two-input carry multiplexer. The 0 input of the carry multiplexer is coupled to a G input. A CI input is coupled to the other input of the XOR gate and to the 1 input of the carry multiplexer.

摘要翻译： 用于FPGA的逻辑模块包括由2：1多路复用器的N级树形成的LUT。 LUT的N个输入中的每一个连接到树的一个级别中的多路复用器的选择输入。 在树的叶片处的每个数据输入由产生逻辑0或逻辑1的配置存储器单元驱动。在树的最后一级的单个多路复用器的输出形成Y输出，并耦合到 XOR门的一个输入和双输入进位多路复用器的选择输入。 进位多路复用器的0输入耦合到G输入。 CI输入耦合到XOR门的另一个输入端和进位复用器的1个输入端。

公开(公告)号：US20090272914A1

公开(公告)日：2009-11-05

申请号：US12434495

申请日：2009-05-01

申请人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may, for instance, be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The captured and detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection (TIR) methods may benefit from the techniques of the present invention. In addition, the biological samples imaged may be present on the surfaces of the support structure in a random special pattern and need not be at known locations in order for the imaging to be performed.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构例如可以是允许试剂流体流过并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 捕获和检测到的荧光发射辐射然后可用于产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射（TIR）方法均可受益于本发明的技术。 此外，成像的生物样品可以以随机特殊图案存在于支撑结构的表面上，并且不需要在已知位置处以便进行成像。

公开(公告)号：US07589315B2

公开(公告)日：2009-09-15

申请号：US11871085

申请日：2007-10-11

申请人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

发明人： Wenyi Feng ,  Theofilos Kotseroglou ,  Mark Wang ,  Alexander Triener ,  Diping Che ,  Robert Kain

IPC分类号： H01L27/00

CPC分类号： G01N21/6456 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G02B21/0032 ,  G02B21/004

摘要： The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

摘要翻译： 本发明提供了用于以快速扫描速率获得样品的高分辨率图像的成像装置和方法。 具有比垂直尺寸长的水平尺寸的矩形检测器阵列可以与定位成将样本的一部分的矩形图像引导到矩形检测器阵列的成像光学元件一起使用。 扫描装置可以被配置成以扫描轴尺寸扫描样本，其中矩形检测器阵列的垂直尺寸和图像的两个矩形尺寸中的较短的尺寸在扫描轴尺寸上，并且其中垂直尺寸 因为矩形检测器阵列足够短以在单个轴上实现共焦。