公开(公告)号：US09110075B2

公开(公告)日：2015-08-18

申请号：US13252778

申请日：2011-10-04

Applicant: Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

Inventor： Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

IPC: G01N33/533 ,  C07K1/13 ,  G01N33/68

CPC classification number: G01N33/6842 ,  C07K16/00 ,  C07K2317/40 ,  G01N33/582 ,  G01N33/6803

Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.

Abstract translation: 公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。

公开(公告)号：US20090011432A1

公开(公告)日：2009-01-08

申请号：US12177049

申请日：2008-07-21

Applicant: Po-Ying Chan-Hui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakosssian ,  Tracy J. Matray

Inventor： Po-Ying Chan-Hui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakosssian ,  Tracy J. Matray

IPC: G01N33/53 ,  G01N33/573

CPC classification number: G01N33/542 ,  G01N33/566 ,  G01N2333/71 ,  G01N2333/726 ,  Y10S436/824

Abstract: Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.

Abstract translation: 提供了用于检测样品（例如细胞或组织裂解物）中分子复合物（特别是蛋白质）的形成的方法。 在一个方面，提供了对复合物中的第一种蛋白质特异性的切割探针和一种或多种特异于复合物中的一种或多种第二种蛋白质的结合化合物。 在结合时，诱导切割探针以产生仅在切割探针的局部区域中切割附着于结合化合物的分子标签的活性物质，例如单线态氧。 将释放的分子标签与测定混合物和彼此分离，以提供与复合物中存在的蛋白质的数量和类型相关的读数。

公开(公告)号：US20150080564A1

公开(公告)日：2015-03-19

申请号：US14343607

申请日：2012-09-07

Applicant: Tracy J. Matray ,  Iwona M. Maciagiewicz ,  Michael E. Houston, JR.

Inventor： Tracy J. Matray ,  Iwona M. Maciagiewicz ,  Michael E. Houston, JR.

IPC: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H23/00 ,  C12N15/113

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H23/00 ,  C12N15/113

Abstract: Synthesis and uses of conformationally restricted nucleomonomers (CRN) to prepare nucleic acid compounds. Methods for preparing nucleomonomers for nucleic acid compounds in high yields and in multi-gram scale for therapeutic modalities useful for treating or preventing diseases or disorders by up- or down-regulating the expression of genes and other nucleic acid based regulatory systems in a cell.

Abstract translation: 构象限制性核单体（CRN）的合成和用途，用于制备核酸化合物。 用于通过上调或下调细胞中基因和其他基于核酸的调节系统的表达来治疗或预防疾病或病症的治疗方法，以高产量和多克量表制备用于核酸化合物的核单体的方法。

公开(公告)号：US09751909B2

公开(公告)日：2017-09-05

申请号：US14343607

申请日：2012-09-07

Applicant: Tracy J. Matray ,  Iwona M. Maciagiewicz ,  Michael E. Houston, Jr.

Inventor： Tracy J. Matray ,  Iwona M. Maciagiewicz ,  Michael E. Houston, Jr.

IPC: C07H21/04 ,  C07H19/04 ,  C07H19/16 ,  C07H19/00 ,  C07H19/06 ,  C07H23/00 ,  C07H19/10 ,  C12N15/113

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H23/00 ,  C12N15/113

Abstract: Processes for synthesis of conformationally restricted nucleomonomers (CRN) by ozonolysis or oxidation of a 2′-vinyl substituted nucleoside precursor with protected 3′-hydroxyl, 4′-hydroxymethyl and 5′-hydroxyl positions. The CRN nucleomonomers can be used to prepare nucleic acid compounds. CRN nucleomonomers for nucleic acid compounds can be prepared in high yields and in multi-gram scale. The nucleic acid compounds can be of various regulatory classes such as antisense RNA for therapeutic modalities useful for treating or preventing diseases or disorders by up- or down-regulating the expression of genes and other nucleic acid based regulatory systems in a cell.

公开(公告)号：US20120088307A1

公开(公告)日：2012-04-12

申请号：US13252778

申请日：2011-10-04

Applicant: Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

Inventor： Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

IPC: G01N27/00

CPC classification number: G01N33/6842 ,  C07K16/00 ,  C07K2317/40 ,  G01N33/582 ,  G01N33/6803

Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.

Abstract translation: 公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。

公开(公告)号：US07402397B2

公开(公告)日：2008-07-22

申请号：US10916291

申请日：2004-08-10

Applicant: Po-Ying Chan-Hui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakosssian ,  Tracy J. Matray

Inventor： Po-Ying Chan-Hui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakosssian ,  Tracy J. Matray

IPC: G01N33/53 ,  G01N33/532 ,  G01N33/554 ,  C07K1/10 ,  C07K1/14

CPC classification number: G01N33/542 ,  G01N33/566 ,  G01N2333/71 ,  G01N2333/726 ,  Y10S436/824

Abstract: Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.

公开(公告)号：US07255999B2

公开(公告)日：2007-08-14

申请号：US10154042

申请日：2002-05-21

Applicant: Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

Inventor： Sharat Singh ,  Hossein Salimi-Moosavi ,  Syed Hasan Tahir ,  Gerald J. Wallweber ,  Hrair Kirakossian ,  Tracy J. Matray ,  Vincent S. Hernandez

IPC: G01N33/53 ,  G01N33/533 ,  C12Q1/42

CPC classification number: G01N33/6842 ,  C07K16/00 ,  C07K2317/40 ,  G01N33/582 ,  G01N33/6803

Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

Abstract translation: 公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。 包含样品和包含切割诱导部分的第一试剂和用于靶多肽上的结合位点的第一结合剂的混合物经受各自结合部分的结合发生的条件。 结合位点是涉及靶多肽的翻译后修饰活性的结果。 该方法可用于确定靶多肽本身。 在另一个实施方案中，靶多肽的存在和/或量与试剂例如涉及靶多肽的翻译后修饰的酶的存在和/或量和/或活性有关。 第一结合剂和结合位点之间的相互作用使切割诱导部分紧密接近可切割部分，其可与多肽相关，并且仅在接近切割诱导部分时易于切割。 以这种方式，可以释放每个多肽的电泳标签。 分离释放的电泳标签，并且基于相应的电泳标签确定靶多肽的存在和/或量。

公开(公告)号：US06649351B2

公开(公告)日：2003-11-18

申请号：US10008593

申请日：2001-11-09

Applicant: Tracy J. Matray ,  Vincent S. Hernandez ,  Ahmed Chenna ,  Herbert Hooper ,  Sharat Singh

Inventor： Tracy J. Matray ,  Vincent S. Hernandez ,  Ahmed Chenna ,  Herbert Hooper ,  Sharat Singh

IPC: C12Q168

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/6816 ,  C40B20/08 ,  C40B40/08 ,  C40B50/16 ,  C40B70/00 ,  G01N33/561 ,  C12Q2521/319 ,  C12Q2563/131 ,  C12Q2565/627

Abstract: The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first binding reagents containing a cleavage-inducing moiety and a target binding moiety, each of the second binding reagents containing a tagged probe having a mass modifier region attached to a target binding moiety by a cleavable linkage, the cleavable linkage being susceptible to cleavage when in proximity to an activated cleavage-inducing moiety; (b) activating the cleavage-inducing moiety to release a tag reporter, and (c) detecting a mass of the tag reporter, the mass uniquely corresponding to a known target analyte.