公开(公告)号：US07037654B2

公开(公告)日：2006-05-02

申请号：US10011201

申请日：2001-11-09

Applicant: Ahmed Chenna ,  Sharat Singh

Inventor： Ahmed Chenna ,  Sharat Singh

IPC: C12Q1/68 ,  C07K7/00 ,  A23J1/00 ,  C07H21/04 ,  C25D17/00

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/6818 ,  C40B20/08 ,  C40B40/08 ,  C40B50/16 ,  C40B70/00 ,  G01N33/561 ,  C12Q2521/319 ,  C12Q2563/131 ,  C12Q2565/30

Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Abstract translation: 提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。 检测涉及到识别标签作为目标识别的结果。 探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。 这些探针包括与位于探针中的切割部分相邻的交互功能，使得交互功能不构成电子标签记录器的一部分。 将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。 将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

公开(公告)号：US06916612B2

公开(公告)日：2005-07-12

申请号：US09825246

申请日：2001-04-02

Applicant: Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

Inventor： Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

IPC: C12N15/09 ,  C07B61/00 ,  C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  B01D57/02 ,  C07H21/04

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C40B70/00 ,  G01N33/561 ,  Y10S435/81

Abstract: Probe sets for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or “e-tag probes”, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Abstract translation: 提供了用于已知选择的核苷酸靶序列的多路检测的探针组。 检测涉及到识别标签作为目标识别的结果。 探针组包括电泳标签探针或“e-标签探针”，其包含与靶结合部分连接的检测区域和称为迁移率调节剂的迁移率限定区域。 e-标签探针的靶结合部分与互补靶序列杂交，随后是e-标签探针的核酸酶切割和可检测的电子标签或电子标签记录的释放。 将混合物暴露于捕获剂，其结合未切割和/或部分切割的e-标签探针，然后进行电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

公开(公告)号：US06632630B2

公开(公告)日：2003-10-14

申请号：US09820289

申请日：2001-03-28

Applicant: Sharat Singh ,  Ahmed Chenna ,  Hossein Salimi-Moosari ,  Ian Gibbons

Inventor： Sharat Singh ,  Ahmed Chenna ,  Hossein Salimi-Moosari ,  Ian Gibbons

IPC: C12Q126

CPC classification number: C12Q1/26 ,  G01N2333/795 ,  G01N2333/80 ,  G01N2333/90245 ,  Y10S435/968

Abstract: Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.

Abstract translation: 使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基和荧光肼。 向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。 该方法可用于评价化合物的酶调节活性

公开(公告)号：US08357277B2

公开(公告)日：2013-01-22

申请号：US12744749

申请日：2008-11-26

Applicant: Youssouf Badal ,  Ahmed Chenna ,  Syed Hasan Tahir ,  Yuping Tan

Inventor： Youssouf Badal ,  Ahmed Chenna ,  Syed Hasan Tahir ,  Yuping Tan

IPC: G01N27/447

CPC classification number: G01N33/532 ,  G01N33/533

Abstract: The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample.

Abstract translation: 本发明涉及检测和/或定量样品中至少一种分析物的增强方法。

公开(公告)号：US20050053939A1

公开(公告)日：2005-03-10

申请号：US10494879

申请日：2002-11-08

Applicant: Ahmed Chenna ,  Sharat Singh

Inventor： Ahmed Chenna ,  Sharat Singh

IPC: G01N33/532 ,  C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/532

Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region, both linked to a target-binding moiety. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Also described are biopolymers and nucleosides containing such interactive functionalities.

Abstract translation: 提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。 检测涉及到识别标签作为目标识别的结果。 探针组包括电泳标签探针或e-标签探针，其包含与靶结合部分连接的检测区域和迁移率定义区域。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。 这些探针包括与位于探针中的切割部分相邻的交互功能，使得交互功能不构成电子标签记录器的一部分。 还描述了含有这种相互作用功能的生物聚合物和核苷。

公开(公告)号：US20050048553A1

公开(公告)日：2005-03-03

申请号：US10918876

申请日：2004-08-16

Applicant: Ahmed Chenna ,  Tracy Matray ,  Vincent Hernandez ,  Herbert Hooper ,  Sharat Singh

Inventor： Ahmed Chenna ,  Tracy Matray ,  Vincent Hernandez ,  Herbert Hooper ,  Sharat Singh

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/58 ,  C12Q1/6809 ,  G01N33/6845 ,  G01N2030/027 ,  C12Q2565/102 ,  C12Q2565/137

Abstract: Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositions and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.

Abstract translation: 公开了用于以同质或非均相测定形式测定样品中一种或多种目标分析物的方法和试剂盒，其使用通过可切割键连接分子标签的结合组合物。 通常，在允许在结合组合物和分析物之间形成稳定的复合物的条件下，形成包含样品和包含多种这样的结合组合物的试剂的测定混合物。 在本发明的一个方面，结合组合物和它们各自的结合位点之间的相互作用使切割诱导部分靠近可裂解连接，或为切割诱导部分提供可识别的底物。 以这种方式，每个分析物的一个或多个分子标签从配合物中释放出来。 释放的分子标签被色谱分离，并且基于释放和分离的分子标签的分析确定靶分析物的存在和/或量。

公开(公告)号：US06770439B2

公开(公告)日：2004-08-03

申请号：US09825244

申请日：2001-04-02

Applicant: Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

Inventor： Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

IPC: C12Q168

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C40B70/00 ,  G01N33/561 ,  Y10S435/81

Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Abstract translation: 提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。 检测涉及到识别标签作为目标识别的结果。 探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。 将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。 将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

公开(公告)号：US10538549B2

公开(公告)日：2020-01-21

申请号：US14659588

申请日：2015-03-16

Applicant: Bo Hang ,  Ahmed Chenna

Inventor： Bo Hang ,  Ahmed Chenna

IPC: G01N33/53 ,  C07H19/23 ,  C07H19/16

Abstract: NNA-derived specific adducts represent an integrated biomarker of exposure to thirdhand smoke (THS) as NNA is unique to THS. The NNA-dG covalent binding adduct could serve as such a biomarker, and play a role in identifying individuals exposed to THS, thus providing critical information for early detection and prevention.

公开(公告)号：US20110180408A1

公开(公告)日：2011-07-28

申请号：US12744749

申请日：2008-11-26

Applicant: Youssouf Badal ,  Ahmed Chenna ,  Syed Hasan Tahir ,  Yuping Tang

Inventor： Youssouf Badal ,  Ahmed Chenna ,  Syed Hasan Tahir ,  Yuping Tang

IPC: G01N27/447

CPC classification number: G01N33/532 ,  G01N33/533

Abstract: The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample.

Abstract translation: 本发明涉及检测和/或定量样品中至少一种分析物的增强方法。

公开(公告)号：US07001725B2

公开(公告)日：2006-02-21

申请号：US09824851

申请日：2001-04-02

Applicant: Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

Inventor： Sharat Singh ,  Tracy Matray ,  Ahmed Chenna

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  B65D85/42 ,  G01N27/26

CPC classification number: C07H19/10 ,  C07H19/06 ,  C07H21/00 ,  Y10S435/808

Abstract: Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, a capture agent and optionally a cleaving agent. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Abstract translation: 提供了用于多个检测一种或多种配体和靶抗配糖体的结合或相互作用的试剂盒。 检测涉及到识别标签作为目标识别的结果。 试剂盒包括电泳标签探针或电子标签探针，捕获剂和任选的裂解剂。 电子标签探针包括检测区域和称为迁移率调节剂的迁移率限定区域，两者都与靶结合部分连接。 在使用试剂盒时，将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。 将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。