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公开(公告)号:US06437141B2
公开(公告)日:2002-08-20
申请号:US09824316
申请日:2001-04-02
申请人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号: C07D27904
CPC分类号: A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译: 具有化学式R 1 -R 8的花青染料各自独立地选自氢,C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇,胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号:US06224644B1
公开(公告)日:2001-05-01
申请号:US09448242
申请日:1999-11-24
申请人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号: C07D27904
CPC分类号: A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译: 具有化学式R 1 -R 8的花青染料各自独立地选自氢,C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇,胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号:US5767287A
公开(公告)日:1998-06-16
申请号:US869032
申请日:1997-06-04
IPC分类号: G01N33/533 , C07D209/14 , C09B23/00 , C09B23/02 , G01N21/78 , C07D209/08
CPC分类号: C09B23/02 , C07D209/14
摘要: Novel intermediates having an indolenine nucleus which are useful in the synthesis of fluorescent sulfoindocyanine dyes are described. Dyes synthesized using such intermediates do not contain a reactive group that will covalently attach to a target molecule at an amine- or hydroxy-containing site. Rather, these intermediates are linked to an enzyme substrate or a member of a specific binding pair. Also included are tyramide-containing sulfonidocyanine dyes.
摘要翻译: 描述了可用于合成荧光磺基吲哚菁染料的具有吲哚宁核的新型中间体。 使用这种中间体合成的染料不含有将在含胺或羟基的位点共价连接到靶分子上的反应性基团。 相反,这些中间体连接到酶底物或特异性结合对的成员。 还包括含有酪酰胺的磺胺蓝染料。
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公开(公告)号:US5688966A
公开(公告)日:1997-11-18
申请号:US687853
申请日:1996-07-26
IPC分类号: G01N33/533 , C07D209/14 , C09B23/00 , C09B23/02 , G01N21/78 , C07D403/06
CPC分类号: C09B23/02 , C07D209/14
摘要: Novel intermediates having an indolenine nucleus which are useful in the synthesis of fluorescent sulfoindocyanine dyes are described. Dyes synthesized using such intermediates do not contain a reactive group that will covalently attach to a target molecule at an amine- or hydroxy-containing site. Rather, these intermediates are linked to an enzyme substrate or a member of a specific binding pair. Also included are tyramide-comaining sulfonidocyanine dyes.
摘要翻译: 描述了可用于合成荧光磺基吲哚菁染料的具有吲哚宁核的新型中间体。 使用这种中间体合成的染料不含有将在含胺或羟基的位点共价连接到靶分子上的反应性基团。 相反,这些中间体连接到酶底物或特异性结合对的成员。 还包括酪酰胺接枝磺胺蓝染料。
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公开(公告)号:US6114350A
公开(公告)日:2000-09-05
申请号:US294678
申请日:1999-04-19
申请人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号: G01N33/533 , A61K49/00 , C07D209/08 , C09B23/00 , C09K11/06 , A61K31/47 , A61K31/425 , C07D215/04 , C07D277/60 , C07D279/16
CPC分类号: A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要: A cyanine dye having the formula ##STR1## wherein R.sub.1 -R.sub.8 are each independently selected from a group consisting of hydrogen, C.sub.1 -C.sub.6 alkyl group, and C.sub.0 -C.sub.4 alkyl group having a hydrophilic substituent thereon. R.sub.11 and R.sub.12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译: 具有下式的花青染料其中R 1 -R 8各自独立地选自氢,C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇,胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号:US06204389B1
公开(公告)日:2001-03-20
申请号:US09448241
申请日:1999-11-24
申请人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号: C07D27904
CPC分类号: A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要: A cyanine dye having the formula wherein R1--R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译: 具有式R 1 -R 8的花青染料各自独立地选自氢,C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇,胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号:US06197956B1
公开(公告)日:2001-03-06
申请号:US09449333
申请日:1999-11-24
申请人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人: Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号: C07D27916
CPC分类号: A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
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