公开(公告)号：US6114350A

公开(公告)日：2000-09-05

申请号：US294678

申请日：1999-04-19

申请人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

发明人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

IPC分类号： G01N33/533 ,  A61K49/00 ,  C07D209/08 ,  C09B23/00 ,  C09K11/06 ,  A61K31/47 ,  A61K31/425 ,  C07D215/04 ,  C07D277/60 ,  C07D279/16

CPC分类号： A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  C07D209/08

摘要： A cyanine dye having the formula ##STR1## wherein R.sub.1 -R.sub.8 are each independently selected from a group consisting of hydrogen, C.sub.1 -C.sub.6 alkyl group, and C.sub.0 -C.sub.4 alkyl group having a hydrophilic substituent thereon. R.sub.11 and R.sub.12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.

摘要翻译： 具有下式的花青染料其中R 1 -R 8各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。

公开(公告)号：US06197956B1

公开(公告)日：2001-03-06

申请号：US09449333

申请日：1999-11-24

申请人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

发明人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

IPC分类号： C07D27916

CPC分类号： A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  C07D209/08

摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.

公开(公告)号：US06437141B2

公开(公告)日：2002-08-20

申请号：US09824316

申请日：2001-04-02

申请人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

发明人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

IPC分类号： C07D27904

CPC分类号： A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  C07D209/08

摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.

摘要翻译： 具有化学式R 1 -R 8的花青染料各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。

公开(公告)号：US06224644B1

公开(公告)日：2001-05-01

申请号：US09448242

申请日：1999-11-24

申请人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

发明人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

IPC分类号： C07D27904

CPC分类号： A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  C07D209/08

摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.

摘要翻译： 具有化学式R 1 -R 8的花青染料各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。

公开(公告)号：US06204389B1

公开(公告)日：2001-03-20

申请号：US09448241

申请日：1999-11-24

申请人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

发明人： Malcolm Harry Randall ,  Philip Richard Buzby ,  Thomas Joseph Erickson ,  Joseph David Trometer ,  Joseph John Miller, Jr. ,  David George Ahern ,  Mark Norman Bobrow

IPC分类号： C07D27904

CPC分类号： A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  C07D209/08

摘要： A cyanine dye having the formula wherein R1--R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.

摘要翻译： 具有式R 1 -R 8的花青染料各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。