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1.发明授权Methods for synthesizing glycinols, glyceollins I and II, compositions of selected intermediates, and therapeutic uses thereof 失效大豆醇，glyceollins I和II的合成方法，所选中间体的组成及其治疗用途公开(公告)号：US08563599B2
公开(公告)日：2013-10-22
申请号：US12921013
申请日：2009-03-03
申请人： Paul W. Erhardt , Rahul Khupse , Jefferey G. Sarver , Thomas E. Cleveland , Stephen M. Boue , Thomas E. Wiese , Matthew E. Burow , John A. McLachlan
发明人： Paul W. Erhardt , Rahul Khupse , Jefferey G. Sarver , Thomas E. Cleveland , Stephen M. Boue , Thomas E. Wiese , Matthew E. Burow , John A. McLachlan
IPC分类号： A61K31/352
CPC分类号： C07D493/14
摘要： Two distinct methods are disclosed and claimed for synthesizing glyceollin I plus glyceollin II as a mixture and as their pure forms. Stereochemical isomers and various synthetic intermediates are also synthesized and claimed for their novel compositions of matter. All compounds and their mixtures are claimed for use in formulations that are useful to treat or prevent cancer, or that have utility as selective estrogen receptor modulators, such formulations including enhanced or medical foods, dietary supplements and ethical pharmaceutical agents.
摘要翻译： 公开和要求的两种不同的方法用于合成甘氨酸蛋白I加血糖素II作为混合物，并以其纯形式。 立体化学异构体和各种合成中间体也由它们的新物质组合物合成和要求保护。 要求所有化合物及其混合物用于可用于治疗或预防癌症或具有选择性雌激素受体调节剂的制剂的制剂中，所述制剂包括增强型或医用食品，膳食补充剂和伦理药剂。
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2.发明申请Method and detection system for detection of aflatoxin in corn with fluorescence spectra 有权用荧光光谱检测玉米黄曲霉毒素的方法和检测系统公开(公告)号：US20120061586A1
公开(公告)日：2012-03-15
申请号：US12807673
申请日：2010-09-10
CPC分类号： G01N21/6456 , G01N21/6486 , G01N2021/6423
摘要： A system and method for fluorescence spectral imaging of target material to detect the presence of a contaminant (such as aflatoxin in corn) is provided. An ultraviolet light source is coupled a light-excluding compartment. The fluorescence from the UV excited target passes through a filter (liquid crystal tunable, acoustic-optic tunable, a filter wheel, or other wavelength splitting device) and a lens, to a spectral imaging camera. Fluorescence spectral image data from the camera is analyzed by a computer and presented in human-readable form. Aflatoxin detection in contaminated corn kernels is based on peak fluorescence and peak fluorescence shift in the spectral range from 451 nm to 500 nm. Aflatoxin contamination level within the target material is quantified based on peak fluorescence and peak fluorescence shift and computed corn kernel pixel statistics.
摘要翻译： 提供了用于检测污染物（例如玉米中的黄曲霉毒素）的存在的靶材料的荧光光谱成像的系统和方法。 紫外线光源与光排除隔间相连。 来自UV激发目标的荧光通过滤光器（液晶可调谐，声光可调谐，滤光轮或其它波长分离装置）和透镜到光谱成像相机。 来自相机的荧光光谱图像数据由计算机分析，并以人类可读的形式呈现。 受污染的玉米粒子中的黄曲霉毒素检测是基于从451nm到500nm的光谱范围内的峰值荧光和峰值荧光移动。 目标材料中的黄曲霉毒素污染水平基于峰荧光和峰值荧光偏移和计算的玉米粒子像素统计量进行定量。
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3.发明申请METHODS FOR SYNTHESIZING GLYCINOLS, GLYCEOLLINS I AND II, COMPOSITIONS OF SELECTED INTERMEDIATES, AND THERAPEUTIC USES THEREOF 失效合成甘氨醇，甘氨酸I和II的方法，选择的中间体的组合物及其治疗用途公开(公告)号：US20110144195A1
公开(公告)日：2011-06-16
申请号：US12921013
申请日：2009-03-03
申请人： Paul W. Erhardt , Rahul Khupse , Jeffrerey G. Sarver , Thomas E. Cleveland , Stephen M. Boue , Thomas E. Wiese , Matthew E. Burow , John A. McClachlan
发明人： Paul W. Erhardt , Rahul Khupse , Jeffrerey G. Sarver , Thomas E. Cleveland , Stephen M. Boue , Thomas E. Wiese , Matthew E. Burow , John A. McClachlan
IPC分类号： A61K31/352 , A61K31/35 , A61K31/12 , C07D311/78 , C07D311/04 , C07C49/825 , A61P43/00
CPC分类号： C07D493/14
摘要： Two distinct methods are disclosed and claimed for synthesizing glyceollin I plus glyceollin II as a mixture and as their pure forms. Stereochemical isomers and various synthetic intermediates are also synthesized and claimed for their novel compositions of matter. All compounds and their mixtures are claimed for use in formulations that are useful to treat or prevent cancer, or that have utility as selective estrogen receptor modulators, such formulations including enhanced or medical foods, dietary supplements and ethical pharmaceutical agents.
摘要翻译： 公开和要求的两种不同的方法用于合成甘氨酸蛋白I加血糖素II作为混合物，并以其纯形式。 立体化学异构体和各种合成中间体也由它们的新物质组合物合成和要求保护。 要求所有化合物及其混合物用于可用于治疗或预防癌症或具有选择性雌激素受体调节剂的制剂的制剂中，所述制剂包括增强型或医用食品，膳食补充剂和伦理药剂。
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4.发明申请Antiestrogenic glyceollins suppress human breast and ovarian carcinoma proliferation and tumorigenesis 有权抗雌激素性糖蛋白抑制人乳腺癌和卵巢癌的增殖和肿瘤发生公开(公告)号：US20080200537A1
公开(公告)日：2008-08-21
申请号：US12082583
申请日：2008-04-11
CPC分类号： A61K36/48
摘要： The flavonoid family of phytochemicals, particularly those derived from soy, has received attention regarding their hormonal activity and their effects on human health and disease. The types and amounts of these compounds in soy and other plants are controlled by both constitutive expression and stress-induced biosynthesis. The health benefits of soy may therefore be dependent upon the amounts of the various hormonally active phytochemicals present. We have identified increased biosynthesis of the isoflavonoid phytoalexin compounds, Glyceollins I, II and III, in soy plants grown under stressed conditions (elicited soy), which exhibit marked anti-estrogenic effects on ER function. Here we demonstrate that specific glyceollins, isolated from elicited soy, displayed anti-estrogenic activity, suppressing basal and estrogen stimulated colony formation of ER-positive estrogen dependent breast cancer cells and inhibiting ER-dependent gene expression of progesterone receptor (PgR) and stromal derived factor-1 (SDF1/CXCL12). Examining the effects of glyceollin on in vivo tumor formation/growth we demonstrate the ability of glyceollins to significantly suppress basal and estrogen-stimulated tumor growth of ER-positive MCF-7 breast and BG-1 ovarian carcinoma cells in ovariectomized female nude mice. We further demonstrate that the effects of glyceollins on suppression of tumor growth correlate with inhibition of estrogen stimulated PgR expression. In contrast to the uterotropic activity of tamoxifen the glyceollins displayed no uterine agonist activity. The Glyceollin (I-III) compounds may represent an important component of the health effects of soy as well as represent novel anti-estrogens useful in the prevention or treatment of breast and ovarian carcinoma.
摘要翻译： 植物化学成分的黄酮类化合物，特别是那些衍生自大豆的类黄酮，已经受到关于其激素活性及其对人类健康和疾病的影响的关注。 这些化合物在大豆和其他植物中的类型和数量都受到组成型表达和应激诱导的生物合成的控制。 因此，大豆的健康益处可能取决于存在的各种激素活性植物化学物质的量。 我们已经确定了在胁迫条件下生长的大豆植物（引发的大豆）中异黄酮类植物抗坏血酸化合物Glyceollins I，II和III的生物合成的增加，其对ER功能表现出显着的抗雌激素作用。 在这里我们证明，从引出的大豆中分离的特定的果糖显示出抗雌激素活性，抑制ER阳性雌激素依赖性乳腺癌细胞的基础和雌激素刺激的集落形成，并抑制孕激素受体（PgR）和基质衍生的ER依赖性基因表达 因子-1（SDF1 / CXCL12）。 检查血糖浓度对体内肿瘤形成/生长的影响，我们证明了血清蛋白在卵巢切除的雌性裸鼠中显着抑制ER阳性MCF-7乳腺和BG-1卵巢癌细胞的基础和雌激素刺激的肿瘤生长的能力。 我们进一步证明，糖蛋白对抑制肿瘤生长的影响与抑制雌激素刺激的PgR表达有关。 与他莫昔芬的子宫活动相反，glyceollins不显示子宫激动剂活性。 Glyceollin（I-III）化合物可能代表大豆健康影响的重要组成部分，也可代表用于预防或治疗乳腺癌和卵巢癌的新型抗雌激素。
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5.发明申请Method and apparatus for non-invasive rapid fungal specie (mold) identification having hyperspectral imagery 审中-公开用于具有高光谱图像的非侵入性快速真菌（霉菌）鉴定的方法和装置公开(公告)号：US20080102487A1
公开(公告)日：2008-05-01
申请号：US11590747
申请日：2006-11-01
CPC分类号： G01N21/31
摘要： In a method and apparatus for identifying and distinguishing fungal species, a hyperspectral imaging scanner is used to acquire hyperspectral image data for radiation obtained from a sample area in which at least one unknown fungal species is present. A computer compares the acquired hyperspectral image data with spectral signature data stored in a digital library, which includes spectral signature data for each one of a group of known fungal species, and identifies the fungal species, based on the result of such comparison. The spectral signature data stored in the digital library take into account, for each fungal species, spectral variations that can occur due to at least one of environmental and temporal influences. The computer comparison includes a pixel-by-pixel analysis of the degree of difference between acquired hyperspectral image data and the spectral signature data, so that a spatial distribution of identified fungal species can be determined for a sample area.
摘要翻译： 在用于识别和区分真菌物种的方法和装置中，使用高光谱成像扫描仪来获取从存在至少一种未知真菌物种的样品区获得的辐射的高光谱图像数据。 计算机将获取的高光谱图像数据与存储在数字图书馆中的光谱签名数据进行比较，该数据库包括一组已知真菌物种中的每一个的光谱特征数据，并且基于这种比较的结果识别真菌物种。 存储在数字图书馆中的光谱特征数据考虑到每个真菌种类，由于环境和时间影响中的至少一种而可能发生的光谱变化。 计算机比较包括对获取的高光谱图像数据和光谱特征数据之间的差异程度的逐像素分析，从而可以为样本区域确定所鉴定真菌物种的空间分布。
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6.发明授权Method and detection system for detection of aflatoxin in corn with fluorescence spectra 有权用荧光光谱检测玉米黄曲霉毒素的方法和检测系统公开(公告)号：US08563934B2
公开(公告)日：2013-10-22
申请号：US12807673
申请日：2010-09-10
IPC分类号： G01J1/58
CPC分类号： G01N21/6456 , G01N21/6486 , G01N2021/6423
摘要： A system and method for fluorescence spectral imaging of target material to detect the presence of a contaminant (such as aflatoxin in corn) is provided. An ultraviolet light source is coupled with a light-excluding compartment. The fluorescence from the UV excited target passes through a filter (liquid crystal tunable, acoustic-optic tunable, a filter wheel, or other wavelength splitting device) and a lens, to a spectral imaging camera. Fluorescence spectral image data from the camera are analyzed by a computer and presented in human-readable form. Aflatoxin detection in contaminated corn kernels is based on peak fluorescence and peak fluorescence shift in the spectral range from 451 nm to 500 nm. Aflatoxin contamination level within the target material is quantified based on peak fluorescence and peak fluorescence shift and computed corn kernel pixel statistics.
摘要翻译： 提供了用于检测污染物（例如玉米中的黄曲霉毒素）的存在的靶材料的荧光光谱成像的系统和方法。 紫外光源与不包括光的隔间结合。 来自UV激发目标的荧光通过滤光器（液晶可调谐，声光可调谐，滤光轮或其它波长分离装置）和透镜到光谱成像相机。 来自相机的荧光光谱图像数据由计算机分析并以人类可读的形式呈现。 受污染的玉米粒子中的黄曲霉毒素检测是基于从451nm到500nm的光谱范围内的峰值荧光和峰值荧光移动。 目标材料中的黄曲霉毒素污染水平基于峰荧光和峰值荧光偏移和计算的玉米粒子像素统计量进行定量。
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公开(公告)号：US20130041022A1
公开(公告)日：2013-02-14
申请号：US13141572
申请日：2009-12-22
IPC分类号： A61K31/35 , C07D493/14 , A61P35/00 , C12N5/09
CPC分类号： A61K31/35 , A61K31/353
摘要： The present disclosure demonstrates the molecular effects of glyceollins on human prostate cancer cell LNCaP to further elucidate its potential effects on prostate cancer prevention. The glyceollins inhibited LNCaP cell growth similar to that of the soy isoflavone genistein. The growth inhibitory effects of the glyceollins appeared to be due to an inhibition on G1/S progression and correlated with an up-regulation of cyclin-dependent kinase inhibitor A1 and B1 mRNA and protein levels. By contrast, genistein only up-regulates cyclin-dependent kinase inhibitor A1. In addition, glyceollin treatments led to down-regulated mRNA levels for androgen responsive genes. In contrast to genistein, this effect of glyceollins on androgen responsive genes appeared to be mediated through modulation of an estrogen- but not androgen-mediated pathway. Hence, the glyceollins exerted multiple effects on LNCaP cells that may be considered cancer preventive and the mechanisms of action appeared to be different from other soy-derived phytochemicals.
摘要翻译： 本发明揭示了glyceollins对人前列腺癌细胞LNCaP的分子效应，以进一步阐明其对前列腺癌预防的潜在影响。 glyceollins抑制与大豆异黄酮染料木素类似的LNCaP细胞生长。 glyceollins的生长抑制作用似乎是由于对G1 / S进展的抑制，与细胞周期蛋白依赖性激酶抑制剂A1和B1 mRNA及蛋白质水平的上调相关。 相比之下，染料木素只上调细胞周期蛋白依赖性激酶抑制剂A1。 此外，甘草素处理导致雄激素反应基因的下调mRNA水平。 与染料木素相反，血凝素对雄激素反应基因的这种影响似乎是通过调节雌激素而不是雄激素介导的途径来介导的。 因此，glyceollins对可能被认为是预防癌症的LNCaP细胞产生多重效应，并且其作用机制似乎与其他大豆衍生的植物化学物质不同。
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8.发明授权Antiestrogenic glyceollins suppress human breast and ovarian carcinoma proliferation and tumorigenesis 有权抗雌激素性糖蛋白抑制人乳腺癌和卵巢癌的增殖和肿瘤发生公开(公告)号：US08323706B2
公开(公告)日：2012-12-04
申请号：US12082583
申请日：2008-04-11
CPC分类号： A61K36/48
摘要： The flavonoid family of phytochemicals, particularly those derived from soy, has received attention regarding their hormonal activity and their effects on human health and disease. The types and amounts of these compounds in soy and other plants are controlled by both constitutive expression and stress-induced biosynthesis. The health benefits of soy may therefore be dependent upon the amounts of the various hormonally active phytochemicals present. We have identified increased biosynthesis of the isoflavonoid phytoalexin compounds, Glyceollins I, II and III, in soy plants grown under stressed conditions (elicited soy), which exhibit marked anti-estrogenic effects on ER function. Here we demonstrate that specific glyceollins, isolated from elicited soy, displayed anti-estrogenic activity, suppressing basal and estrogen stimulated colony formation of ER-positive estrogen dependent breast cancer cells and inhibiting ER-dependent gene expression of progesterone receptor (PgR) and stromal derived factor-1 (SDF1/CXCL12). Examining the effects of glyceollin on in vivo tumor formation/growth we demonstrate the ability of glyceollins to significantly suppress basal and estrogen-stimulated tumor growth of ER-positive MCF-7 breast and BG-1 ovarian carcinoma cells in ovariectomized female nude mice. We further demonstrate that the effects of glyceollins on suppression of tumor growth correlate with inhibition of estrogen stimulated PgR expression. In contrast to the uterotropic activity of tamoxifen the glyceollins displayed no uterine agonist activity. The Glyceollin (I-III) compounds may represent an important component of the health effects of soy as well as represent novel anti-estrogens useful in the prevention or treatment of breast and ovarian carcinoma.
摘要翻译： 植物化学成分的黄酮类化合物，特别是那些衍生自大豆的类黄酮，已经受到关于其激素活性及其对人类健康和疾病的影响的关注。 这些化合物在大豆和其他植物中的类型和数量都受到组成型表达和应激诱导的生物合成的控制。 因此，大豆的健康益处可能取决于存在的各种激素活性植物化学物质的量。 我们已经确定了在胁迫条件下生长的大豆植物（引发的大豆）中异黄酮类植物抗坏血酸化合物Glyceollins I，II和III的生物合成的增加，其对ER功能表现出显着的抗雌激素作用。 在这里我们证明，从引出的大豆中分离的特定的果糖显示出抗雌激素活性，抑制ER阳性雌激素依赖性乳腺癌细胞的基础和雌激素刺激的集落形成，并抑制孕激素受体（PgR）和基质衍生的ER依赖性基因表达 因子-1（SDF1 / CXCL12）。 检查血糖浓度对体内肿瘤形成/生长的影响，我们证明了血清蛋白在卵巢切除的雌性裸鼠中显着抑制ER阳性MCF-7乳腺和BG-1卵巢癌细胞的基础和雌激素刺激的肿瘤生长的能力。 我们进一步证明，糖蛋白对抑制肿瘤生长的影响与抑制雌激素刺激的PgR表达有关。 与他莫昔芬的子宫活动相反，glyceollins不显示子宫激动剂活性。 Glyceollin（I-III）化合物可能代表大豆健康影响的重要组成部分，也可代表用于预防或治疗乳腺癌和卵巢癌的新型抗雌激素。
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9.发明授权Transformation of plants with a chloroperoxidase gene to enhance disease resistance 失效用氯过氧化物酶基因转化植物以增强抗病性公开(公告)号：US06703540B1
公开(公告)日：2004-03-09
申请号：US09522401
申请日：2000-03-09
申请人： Thomas J. Jacks , Jeffrey W. Cary , Kanniah Rajasekaran , Thomas E. Cleveland, III , Karl-Heinz Van Pee
发明人： Thomas J. Jacks , Jeffrey W. Cary , Kanniah Rajasekaran , Thomas E. Cleveland, III , Karl-Heinz Van Pee
IPC分类号： C12N1509
CPC分类号： C12N9/0065 , C12N15/8282
摘要： The present invention provides a method of making plants resistant to plant pathogens by transformation of host cells with a nucleic acid encoding a nonheme haloperoxidase. Transgenic plants which express a nonheme chloroperoxidase thereby resulting in enhanced resistance to phytopathogens are provided.
摘要翻译： 本发明提供了通过用编码非氨基过氧化物酶的核酸转化宿主细胞来制备对植物病原体耐受植物的方法。 提供了表达非化学过氧化物酶从而导致对植物病原体的增强抗性的转基因植物。
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10.发明授权Small peptides with antipathogenic activity, treated plants and methods for treating same 失效具有抗病原活性的小肽，经处理的植物及其处理方法公开(公告)号：US06183736B2
公开(公告)日：2001-02-06
申请号：US09287515
申请日：1999-04-07
IPC分类号： A01N6300
CPC分类号： C07K7/56 , A01N37/46 , A01N63/02 , C07K14/32 , Y10S435/839
摘要： The invention relates to two lipopeptides a1 and a2 produced by Bacillus subtilis and their use as an anitfungal agent against Aspergillus flavus. Both peptides are cyclic, acidic and have broad range of antifungal and antimicrobial activity. Both peptides belong to the Bacillomycin D family. A method and composition for controlling aflatoxin contamination in plants susceptible to alflatoxin-producing fungi, like Aspergillus flavus or Aspergillus parasiticus is also disclosed.
摘要翻译： 本发明涉及由枯草芽孢杆菌生产的两种脂肽a1和a2及其作为抗黄曲霉的抗真菌剂的用途。 这两种肽都是环状，酸性的，具有广泛的抗真菌和抗菌活性。 这两种肽都属于百利霉素D家族。 还公开了一种用于控制对产黄体生物菌（如黄曲霉或寄生曲霉）敏感的植物易感黄曲霉毒素污染的方法和组合物。
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