Process for labeling acridinium to microparticles and application in an
instrument
    4.
    发明授权
    Process for labeling acridinium to microparticles and application in an instrument 失效
    将吖啶标记为微粒并用于仪器中的方法

    公开(公告)号:US5468649A

    公开(公告)日:1995-11-21

    申请号:US196536

    申请日:1994-02-15

    CPC分类号: G01N33/585

    摘要: Embodiments of the invention provide methods of preparing an activated acridinium microparticle. Generally, the methods involve direct covalent coupling or an affinity format. The direct covalent coupling method involves coating a microparticle with a proteinaceous compound. Then, a 10-methyl-N-tosyl-N-(2-carboxyethyl)-9-acridinium carboximide trifluoromethane sulfonate is coupled to the proteinaceous compound. In the affinity format, a microparticle is coated with a biotinylated proteinaceous compound. The microparticle is reacted with an anti-biotin labelled 10-methyl-N-tosyl-N-(2-carboxyethyl)-9-acridinium carboximide trifluoromethane sulfonate. Methods are also provided for using such a microparticle. Those methods of use can estimate transfer efficiency, calibrate optics, and measure membrane pore size of a chemiluminescence based instrument. Test elements for analytical instruments are also provided.

    摘要翻译: 本发明的实施方案提供了制备活化的吖啶微粒的方法。 通常,该方法涉及直接共价偶联或亲和格式。 直接共价偶联法包括用蛋白质化合物包被微粒。 然后,将10-甲基-N-甲苯磺酰基-N-(2-羧乙基)-9-吖啶鎓三氟甲磺酸酯与蛋白质化合物偶联。 在亲和性形式中,微粒被生物素化的蛋白质化合物包被。 将微粒与抗生物素标记的10-甲基-N-甲苯磺酰基-N-(2-羧乙基)-9-吖啶鎓三氟甲磺酸盐反应。 还提供了使用这种微粒的方法。 这些使用方法可以估计传递效率,校准光学和测量基于化学发光的仪器的膜孔径。 还提供了分析仪器的测试元件。

    Confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency
    5.
    发明申请
    Confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency 审中-公开
    共焦显微镜系统用于实时同步时间测量异时波周期和睫状脉搏频率

    公开(公告)号:US20060256342A1

    公开(公告)日:2006-11-16

    申请号:US10550124

    申请日:2004-03-26

    IPC分类号: G01B9/02

    CPC分类号: G02B21/0084 G01N21/49

    摘要: The present invention relates to a method and system that enables continuous real-time analysis of both ciliary beat frequency and metachronal wave frequency from a single spot in excised native ciliated epithelial tissues as well as in primary and subsequent epithelial cultures. Such method and system utilizes the concept of time-scale wavelet analysis and Hilbert Transformation for backscattered light derived from a confocal (conjugate) spot on the moving cilia. This light contains inherent high and low frequency components corresponding to CBF and MWF.

    摘要翻译: 本发明涉及一种方法和系统,其能够在切除的天然纤毛上皮组织以及初级和随后的上皮培养物中从单个斑点连续实时分析睫状节拍频率和异时波频率。 这种方法和系统利用时间尺度小波分析和希尔伯特变换的概念,用于来自移动纤毛上的共焦点(共轭)斑点的背散射光。 该灯含有对应于CBF和MWF的固有高频和低频分量。