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公开(公告)号：US20050025306A1
公开(公告)日：2005-02-03
申请号：US10631914
申请日：2003-07-30
CPC分类号： H04M19/04 , H04M19/042
摘要： Crescendo telephone ringers and methods of providing a crescendo telephone ring signal. The crescendo telephone ringer provides a gradual increase in audible ringing volume as a sequence of incoming electrical ring signals is detected. According to one embodiment, upon receipt of an incoming call, a first audible ring signal corresponding to a first detected electrical ring signal is set to a minimum volume. Subsequent audible ring signals corresponding to subsequent detected electrical ring signals are set to increasing volume levels, and finally to a maximum ringer volume.
摘要翻译： 渐强电话铃声和提供渐强电话铃声信号的方法。 渐强电话铃声提供了可听响铃音量的逐渐增加，因为检测到一系列进入的电环信号。 根据一个实施例，在接收到来电时，对应于第一检测到的电环信号的第一可听振铃信号被设置为最小音量。 对应于随后检测到的电环信号的随后的可听铃信号被设置为增加音量级别，最后设置为最大振铃音量。
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公开(公告)号：US07723103B2
公开(公告)日：2010-05-25
申请号：US11621031
申请日：2007-01-08
IPC分类号： C12N15/74
CPC分类号： C12N15/70
摘要： Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.
摘要翻译： 提供适合用于克隆的载体制备物和克隆构建体。 载体制剂是具有两个3'末端的双链DNA分子，每个末端具有单个碱基对突出端，其能够与待克隆的双链多核苷酸序列上的单个碱基对突出端杂交。 载体制剂的突出端合适地是dCMP，并且待克隆的多核苷酸序列的突出端合适地是dGMP。 在其他实施方案中，要克隆的多核苷酸序列的突出端是任何ddNTP，并且载体制剂的相应突出端是与ddNTP配对的任何碱基。 后一个实施方案特别适用于制备仅具有单个插入物的重组分子。 还提供了克隆方法，制备重组分子文库的方法和用于实施方法的试剂盒。
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公开(公告)号：US20080166773A1
公开(公告)日：2008-07-10
申请号：US11621031
申请日：2007-01-08
CPC分类号： C12N15/70
摘要： Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.
摘要翻译： 提供适合用于克隆的载体制备物和克隆构建体。 载体制剂是具有两个3'末端的双链DNA分子，每个末端具有单个碱基对突出端，其能够与待克隆的双链多核苷酸序列上的单个碱基对突出端杂交。 载体制剂的突出端合适地是dCMP，并且待克隆的多核苷酸序列的突出端合适地是dGMP。 在其他实施方案中，要克隆的多核苷酸序列的突出端是任何ddNTP，并且载体制剂的相应突出端是与ddNTP配对的任何碱基。 后一个实施方案特别适用于制备仅具有单个插入物的重组分子。 还提供了克隆方法，制备重组分子文库的方法和用于实施方法的试剂盒。
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