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公开(公告)号：US5932703A
公开(公告)日：1999-08-03
申请号：US660542
申请日：1996-06-07
申请人： Ronald Godiska , Patrick W. Gray
发明人： Ronald Godiska , Patrick W. Gray
IPC分类号： G01N33/566 , A61K38/00 , A61K39/00 , A61P29/00 , A61P43/00 , C07K14/52 , C07K14/715 , C07K16/24 , C12N5/00 , C12N5/10 , C12N15/09 , C12N15/19 , C12P21/02 , C12P21/08 , C12R1/91 , A61K38/19
CPC分类号： C07K14/7158 , C07K14/523 , A01K2217/05 , A61K38/00 , A61K39/00 , C07K2319/00 , C07K2319/02 , Y10S930/14
摘要： The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C--C chemokine designated MDC, and polypeptide analogs thereof. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein, and polypeptide analogs thereof.
摘要翻译： 本发明提供了编码新的人巨噬细胞衍生的C-C趋化因子（命名为MDC）的纯化和分离的多核苷酸序列及其多肽类似物。 还提供了用于重组生成趋化因子的材料和方法，以及纯化和分离的趋化因子蛋白及其多肽类似物。
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公开(公告)号：US09029134B2
公开(公告)日：2015-05-12
申请号：US12159956
申请日：2007-01-12
申请人： Ronald Godiska , David A. Mead , Nikolai V. Ravin
发明人： Ronald Godiska , David A. Mead , Nikolai V. Ravin
摘要： Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.
摘要翻译： 提供了源自大肠杆菌的噬菌体和适合克隆的宿主细胞的线性载体。 线性载体包括包含左端粒和第一可选择标记的左臂，包含右端粒和第二可选择标记的右臂和位于左臂和右臂之间的克隆区。 载体的任选的其它组分包括转录终止序列，多克隆位点和记录填充区。
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公开(公告)号：US07723103B2
公开(公告)日：2010-05-25
申请号：US11621031
申请日：2007-01-08
IPC分类号： C12N15/74
CPC分类号： C12N15/70
摘要： Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.
摘要翻译： 提供适合用于克隆的载体制备物和克隆构建体。 载体制剂是具有两个3'末端的双链DNA分子，每个末端具有单个碱基对突出端，其能够与待克隆的双链多核苷酸序列上的单个碱基对突出端杂交。 载体制剂的突出端合适地是dCMP，并且待克隆的多核苷酸序列的突出端合适地是dGMP。 在其他实施方案中，要克隆的多核苷酸序列的突出端是任何ddNTP，并且载体制剂的相应突出端是与ddNTP配对的任何碱基。 后一个实施方案特别适用于制备仅具有单个插入物的重组分子。 还提供了克隆方法，制备重组分子文库的方法和用于实施方法的试剂盒。
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公开(公告)号：US20080166773A1
公开(公告)日：2008-07-10
申请号：US11621031
申请日：2007-01-08
CPC分类号： C12N15/70
摘要： Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.
摘要翻译： 提供适合用于克隆的载体制备物和克隆构建体。 载体制剂是具有两个3'末端的双链DNA分子，每个末端具有单个碱基对突出端，其能够与待克隆的双链多核苷酸序列上的单个碱基对突出端杂交。 载体制剂的突出端合适地是dCMP，并且待克隆的多核苷酸序列的突出端合适地是dGMP。 在其他实施方案中，要克隆的多核苷酸序列的突出端是任何ddNTP，并且载体制剂的相应突出端是与ddNTP配对的任何碱基。 后一个实施方案特别适用于制备仅具有单个插入物的重组分子。 还提供了克隆方法，制备重组分子文库的方法和用于实施方法的试剂盒。
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公开(公告)号：US06348574B1
公开(公告)日：2002-02-19
申请号：US09088337
申请日：1998-06-01
IPC分类号： C07K1472
CPC分类号： C07K14/705 , A61K38/00 , A61K2039/505 , C07K16/28 , C07K2319/00
摘要： DNA sequences encoding seven novel seven transmembrane receptors and variants thereof are disclosed as well as materials and methods for production of the same by recombinant techniques. Antibody substances specific for each of the seven transmembrane receptors are disclosed as useful for the modulation of the ligand/receptor binding reactions of the receptors.
摘要翻译： 公开了编码七种新型七个跨膜受体及其变体的DNA序列以及通过重组技术制备其的材料和方法。 公开了对七种跨膜受体中的每一种特异性的抗体物质，用于调节受体的配体/受体结合反应。
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公开(公告)号：US20100255561A1
公开(公告)日：2010-10-07
申请号：US12755235
申请日：2010-04-06
申请人： Eric Steinmetz , Ronald Godiska , David A. Mead
发明人： Eric Steinmetz , Ronald Godiska , David A. Mead
IPC分类号： C12N1/21
摘要： A system for ligase-free cloning and/or expressing a target gene is described herein. A preferred version of the invention includes an E. coli host. The host preferably includes a T7 RNA polymerase gene comprising a T7gpl coding sequence, a lacUV5 promoter, and a lac operator. The host preferably further includes a lacI gene comprising a lacI coding sequence with an ATG start codon, a promoter derived from the lacql allele, and a translational enhancer derived from a 5′ RNA leader sequence of T7 gene 10. The invention further includes a low-copy plasmid vector comprising a T7 promoter a lac operator operationally linked to the T7 promoter. The system is configured to inhibit target gene expression when uninduced and to permit gene expression upon induction by auto-induction.
摘要翻译： 本文描述了用于无连接酶克隆和/或表达靶基因的系统。 本发明的优选方案包括大肠杆菌宿主。 宿主优选包含T7RNA聚合酶基因，其包含T7gpl编码序列，lacUV5启动子和lac操纵子。 宿主优选还包括lacI基因，其包含具有ATG起始密码子的lacI编码序列，来自lacQL等位基因的启动子和源自T7基因10的5'RNA前导序列的翻译增强子。本发明还包括低 -copy质粒载体，其包含T7启动子，与T7启动子可操作地连接的lac操纵子。 该系统配置为在未诱导时抑制靶基因表达，并允许通过自动诱导诱导后的基因表达。
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公开(公告)号：US20090263873A1
公开(公告)日：2009-10-22
申请号：US12159956
申请日：2007-01-12
申请人： Ronald Godiska , David A. Mead , Nikolai V. Ravin
发明人： Ronald Godiska , David A. Mead , Nikolai V. Ravin
摘要： Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.
摘要翻译： 提供了源自大肠杆菌的噬菌体和适合克隆的宿主细胞的线性载体。 线性载体包括包含左端粒和第一可选择标记的左臂，包含右端粒和第二可选择标记的右臂和位于左臂和右臂之间的克隆区。 载体的任选的其它组分包括转录终止序列，多克隆位点和记录填充区。
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公开(公告)号：US06790947B1
公开(公告)日：2004-09-14
申请号：US08479620
申请日：1995-06-07
申请人： Ronald Godiska , Patrick W. Gray
发明人： Ronald Godiska , Patrick W. Gray
IPC分类号： C07H2102
CPC分类号： C07K14/523 , A01K2217/05 , A61K38/00 , A61K39/00 , C07K14/7158 , C07K2319/00 , C07K2319/02
摘要： The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C—C chemokine designated MDC. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein.
摘要翻译： 本发明提供了编码命名为MDC的新型人巨噬细胞衍生C-C趋化因子的纯化和分离的多核苷酸序列。 还提供了用于重组生成趋化因子和纯化和分离的趋化因子蛋白的材料和方法。
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公开(公告)号：US06320023B1
公开(公告)日：2001-11-20
申请号：US08479603
申请日：1995-06-07
申请人： Ronald Godiska , Patrick W. Gray
发明人： Ronald Godiska , Patrick W. Gray
IPC分类号： C07K1452
CPC分类号： C07K14/523 , A61K38/00 , C12N2799/021
摘要： The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C-C chemokine designated MDC. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein.
摘要翻译： 本发明提供了编码命名为MDC的新型人巨噬细胞衍生C-C趋化因子的纯化和分离的多核苷酸序列。 还提供了用于重组生成趋化因子和纯化和分离的趋化因子蛋白的材料和方法。
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公开(公告)号：US6107475A
公开(公告)日：2000-08-22
申请号：US299843
申请日：1999-04-26
IPC分类号： A61K38/00 , C07K14/705 , C07K16/28 , C07H21/04
CPC分类号： C07K16/28 , C07K14/705 , A61K2039/505 , A61K38/00 , C07K2317/34 , C07K2319/00
摘要： DNA sequences encoding seven novel seven transmembrane receptors and variants thereof are disclosed as well as materials and methods for production of the same by recombinant techniques. Antibody substances specific for each of the seven transmembrane receptors are disclosed as useful for the modulation of the ligand/receptor binding reactions of the receptors.
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