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1.发明申请Hybrid automated continuous nucleic acid and protein analyzer using real-time PCR and liquid bead arrays 审中-公开混合自动连续核酸和蛋白质分析仪，使用实时PCR和液珠阵列公开(公告)号：US20050239192A1
公开(公告)日：2005-10-27
申请号：US11159008
申请日：2005-06-21
CPC分类号： G01N1/2202 , G01N1/2211 , G01N15/1459 , G01N35/08 , G01N2001/2217 , Y02A90/26
摘要： A nucleic acid assay system for analyzing a sample using a reagent. A sample and reagent delivery unit is operatively connected to a thermal cycler for delivering the sample and the reagent to the thermal cycler. A hybridization chamber is operatively connected to the thermal cycler. A flow cytometer is operatively connected to the hybridization chamber.
摘要翻译： 一种用于使用试剂分析样品的核酸测定系统。 样品和试剂递送单元可操作地连接到热循环仪，用于将样品和试剂输送到热循环仪。 杂交室可操作地连接到热循环仪。 流式细胞仪可操作地连接到杂交室。
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2.发明授权Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid 有权使用磁珠分析包含核酸的样品的聚合酶链反应体系公开(公告)号：US07867713B2
公开(公告)日：2011-01-11
申请号：US12106390
申请日：2008-04-21
申请人： Shanavaz Nasarabadi
发明人： Shanavaz Nasarabadi
CPC分类号： G01N35/1097 , B01L7/52 , C12Q1/6806 , G01N35/0098
摘要： A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.
摘要翻译： 用于分析含有核酸的样品的聚合酶链反应系统包括提供磁珠; 提供具有聚合酶链式反应室，与聚合酶链式反应室相邻的聚合酶链反应磁体位置的聚合酶链反应室和与聚合酶链反应室相邻的后聚合酶前体位置的流路。 核酸与磁珠结合。 具有核酸的磁珠流动到流路中的预聚合酶链反应磁体位置。 磁珠和核酸用乙醇洗涤。 聚合酶链反应室中的核酸被扩增。 将磁珠和核酸分离成含有磁珠的废物流和含有核酸的聚合酶链反应混合物混合物。 含有核酸的反应混合物流入通道中的分析单元用于分析。
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公开(公告)号：US20090263794A1
公开(公告)日：2009-10-22
申请号：US12106390
申请日：2008-04-21
申请人： Shanavaz Nasarabadi
发明人： Shanavaz Nasarabadi
CPC分类号： G01N35/1097 , B01L7/52 , C12Q1/6806 , G01N35/0098
摘要： A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.
摘要翻译： 用于分析含有核酸的样品的聚合酶链反应系统包括提供磁珠; 提供具有聚合酶链式反应室，与聚合酶链式反应室相邻的聚合酶链反应磁体位置的聚合酶链反应室和与聚合酶链反应室相邻的后聚合酶前体位置的流路。 核酸与磁珠结合。 具有核酸的磁珠流动到流路中的预聚合酶链反应磁体位置。 磁珠和核酸用乙醇洗涤。 聚合酶链反应室中的核酸被扩增。 将磁珠和核酸分离成含有磁珠的废物流和含有核酸的聚合酶链反应混合物混合物。 含有核酸的反应混合物流入通道中的分析单元用于分析。
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公开(公告)号：US20070107538A1
公开(公告)日：2007-05-17
申请号：US11497908
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N15/0606 , C12N1/06 , G01N1/2208 , G01N1/44 , G01N2015/0088 , G01N2015/0261
摘要： A system for sampling air and collecting particles potentially including bioagents entrained in the air for detection. The system comprises collecting a sample of the air with the particles entrained in the air, directing the sample to a receiving surface, directing a liquid to the receiving surface thereby producing a liquid surface, wherein the particles potentially including bioagents become captured in the liquid, and heating the liquid wherein the particles potentially including bioagents become heated to lysis the bioagents.
摘要翻译： 一种用于对空气进行采样和收集颗粒的系统，其中可能包括携带在空气中的生物信号进行检测。 该系统包括用携带在空气中的颗粒收集空气样品，将样品引导到接收表面，将液体引导到接收表面，从而产生液体表面，其中潜在地包括生物标记的颗粒被捕获在液体中， 并加热液体，其中潜在地包括生物体的颗粒被加热以裂解生物制剂。
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公开(公告)号：US20070117110A1
公开(公告)日：2007-05-24
申请号：US11454478
申请日：2006-06-16
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2565/626 , C12Q2563/155 , C12Q2561/101 , C12Q2537/143
摘要： Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.
摘要翻译： 本文公开了用于快速多重分析以确定DNA样品中靶DNA序列的存在和身份的两种方法。 两种方法使用报告DNA序列，例如修饰的常规Taqman探针，以使用流式细胞术检测方法将多重PCR扩增与基于微球的杂交组合。 也可以并入实时PCR检测。 第一种方法使用花青染料，例如Cy3 TM作为连接到报告DNA序列的5'末端的报告物。 第二种方法将报告染料（例如FAM）置于报告DNA序列的3'末端和5'末端的猝灭剂染料（例如TAMRA）上。
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公开(公告)号：US07972818B2
公开(公告)日：2011-07-05
申请号：US11454478
申请日：2006-06-16
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2565/626 , C12Q2563/155 , C12Q2561/101 , C12Q2537/143
摘要： Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM™ on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA™, on the 5′ end.
摘要翻译： 本文公开了用于快速多重分析以确定DNA样品中靶DNA序列的存在和身份的两种方法。 两种方法使用报告DNA序列，例如修饰的常规探针，使用流式细胞术检测方法将多重PCR扩增与基于微球的杂交组合。 也可以并入实时PCR检测。 第一种方法使用花青染料，例如Cy3 TM作为与报告DNA序列的5'末端连接的报告物。 第二种方法将报告染料，例如FAM TM置于报告DNA序列的3'末端，并在5'端定位猝灭剂染料，例如TAMRA TM。
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公开(公告)号：US20070107539A1
公开(公告)日：2007-05-17
申请号：US11497918
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N15/0606 , G01N1/2208 , G01N1/44 , G01N2015/0088 , G01N2015/0261
摘要： A system for sampling air and collecting particles entrained in the air that potentially include bioagents. The system comprises providing a receiving surface, directing a liquid to the receiving surface and producing a liquid surface. Collecting samples of the air and directing the samples of air so that the samples of air with particles entrained in the air impact the liquid surface. The particles potentially including bioagents become captured in the liquid. The air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid but cause minor turbulence. The liquid surface has a surface tension and the collector samples the air and directs the air to the liquid surface so that the air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid, but cause minor turbulence on the surface resulting in insignificant evaporation of the liquid.
摘要翻译： 一种用于对空气进行取样并收集在空气中的颗粒的系统，其中可能包括生物标签。 该系统包括提供接收表面，将液体引导到接收表面并产生液体表面。 收集空气的样品并引导空气样品，使得夹带在空气中的颗粒的空气样品冲击液体表面。 潜在地包括生物标记的颗粒被捕获在液体中。 夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流。 液体表面具有表面张力，并且收集器对空气进行采样并将空气引导到液体表面，使得夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流 在表面上导致液体的微不足道的蒸发。
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公开(公告)号：US20070107537A1
公开(公告)日：2007-05-17
申请号：US11497897
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N1/2208 , G01N2001/2217
摘要： A system for sampling air and collecting particles entrained in the air comprising a receiving surface, a liquid input that directs liquid to the receiving surface and produces a liquid surface, an air input that directs the air so that the air with particles entrained in the air impact the liquid surface, and an electrostatic contact connected to the liquid that imparts an electric charge to the liquid. The particles potentially including bioagents become captured in the liquid by the air with particles entrained in the air impacting the liquid surface. Collection efficiency is improved by the electrostatic contact electrically charging the liquid. The effects of impaction and adhesion due to electrically charging the liquid allows a unique combination in a particle capture medium that has a low fluid consumption rate while maintaining high efficiency.
摘要翻译： 用于对空气进行采样并收集夹带在空气中的颗粒的系统，包括接收表面，将液体引导到接收表面并产生液体表面的液体输入;引导空气的空气输入，使得具有夹带在空气中的颗粒的空气 冲击液体表面，以及连接到液体上的电荷的静电接触。 潜在地包括生物标记的颗粒被空气捕获在液体中，颗粒夹带在空气中，撞击液体表面。 通过使液体充电的静电接触来提高收集效率。 由于对液体进行充电而引起的冲击和粘附的影响允许在保持高效率的同时具有低流体消耗率的颗粒捕获介质中的独特组合。
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公开(公告)号：US20060057599A1
公开(公告)日：2006-03-16
申请号：US11000491
申请日：2004-11-29
申请人： John Dzenitis , Steve Brown , Billy Colston , Dora Gutierrez , Bruce Henderer , Benjamin Hindson , Ramakrishna Madabhushi , Anthony Makarewicz , Don Masquelier , Mary McBride , Thomas Metz , Shanavaz Nasarabadi , Ujwal Setlur , Sally Smith , Kodumudi Venkateswaran
发明人： John Dzenitis , Steve Brown , Billy Colston , Dora Gutierrez , Bruce Henderer , Benjamin Hindson , Ramakrishna Madabhushi , Anthony Makarewicz , Don Masquelier , Mary McBride , Thomas Metz , Shanavaz Nasarabadi , Ujwal Setlur , Sally Smith , Kodumudi Venkateswaran
CPC分类号： G01N1/2202 , G01N1/2211 , G01N15/1459 , G01N35/08 , G01N2001/2217 , Y02A90/26
摘要： An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for detecting said bioagents.
摘要翻译： 一种用于监测用于生物制剂的空气，水，土壤或其他物质的自主监测装置。 收集者收集一定数量的被监测的空气，水，土壤或其他物质。 样品制备系统准备所选择的潜在生物标记颗粒的样品。 通过用于检测所述生物代理的系统分析样品。
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公开(公告)号：US07083951B2
公开(公告)日：2006-08-01
申请号：US10077321
申请日：2002-02-14
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2565/626 , C12Q2563/155 , C12Q2561/101 , C12Q2537/143
摘要： Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.
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