公开(公告)号：US20100239545A9

公开(公告)日：2010-09-23

申请号：US10292951

申请日：2002-11-13

Applicant: William McKay ,  Scott Boden ,  Sangwook Yoon

Inventor： William McKay ,  Scott Boden ,  Sangwook Yoon

IPC: A61K48/00 ,  A61F2/44 ,  C07H21/04 ,  C12N5/08

CPC classification number: A61K48/005 ,  A61K35/12 ,  A61K35/13 ,  A61K48/00 ,  A61K2121/00 ,  A61L27/3804 ,  A61L27/3856 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/78 ,  C07K16/18 ,  C12N2799/022

Abstract: Methods of expressing LIM mineralization protein in non-osseous mammalian cells, such as stem cells or intervertebral disc cells (e.g., cells of the annulus fibrosus, or cells of the nucleus pulposus) are described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Expression of the LIM mineralization protein can stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycyan and/or collagen. Methods for treating disc disease associated with trauma or disc degeneration are also described.

Abstract translation: 描述了在非骨骼哺乳动物细胞中表达LIM矿化蛋白的方法，例如干细胞或椎间盘细胞（例如，纤维环的细胞或髓核的细胞）。 所述方法涉及用分离的核酸转染细胞，所述分离的核酸包含可操作地连接到启动子的编码LIM矿化蛋白的核苷酸序列。 转染可以通过直接注射病毒或裸露DNA或非病毒载体如质粒在体内或体内完成。 LIM矿化蛋白的表达可以刺激能够产生蛋白多糖和/或胶原的细胞中的蛋白聚糖和/或胶原产生。 还描述了与创伤或椎间盘退变相关的椎间盘疾病的治疗方法。

公开(公告)号：US20090304649A9

公开(公告)日：2009-12-10

申请号：US11602805

申请日：2006-11-21

Applicant: William McKay ,  Scott Boden ,  Sangwook Yoon

Inventor： William McKay ,  Scott Boden ,  Sangwook Yoon

IPC: A61K48/00

CPC classification number: A61K38/1841 ,  A61K38/1875 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/51

Abstract: Methods of inducing the expression of a proteoglycan such as aggrecan in a cell are described. A method is described which includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The LIM mineralization protein can be rLMP, hLMP-1, hLMP-1s, or hLMP-3. Transfection maybe accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce proteoglycan synthesis in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycan and/or collagen (e.g., intervertebral disc cells including cells of the nucleus pulposus and annulus fibrosus).

Abstract translation: 描述了在细胞中诱导蛋白聚糖如聚集蛋白聚糖表达的方法。 描述了一种方法，其包括用分离的核酸转染细胞，所述分离的核酸包含可操作地连接于启动子的编码LIM矿化蛋白的核苷酸序列。 LIM矿化蛋白可以是rLMP，hLMP-1，hLMP-1或hLMP-3。 转染可以通过直接注射病毒或裸DNA或通过非病毒载体如质粒在体内或体内完成。 该方法可用于在骨细胞中诱导蛋白聚糖合成或刺激能够产生蛋白聚糖和/或胶原的细胞（例如包括髓核细胞和纤维环的椎间盘细胞）中的蛋白聚糖和/或胶原产生。

公开(公告)号：US20070134218A1

公开(公告)日：2007-06-14

申请号：US11602805

申请日：2006-11-21

Applicant: William McKay ,  Scott Boden ,  Sangwook Yoon

Inventor： William McKay ,  Scott Boden ,  Sangwook Yoon

IPC: A61K48/00

CPC classification number: A61K38/1841 ,  A61K38/1875 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/51

Abstract: Methods of inducing the expression of a proteoglycan such as aggrecan in a cell are described. A method is described which includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The LIM mineralization protein can be rLMP, hLMP-1, hLMP-1s, or hLMP-3. Transfection maybe accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce proteoglycan synthesis in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycan and/or collagen (e.g., intervertebral disc cells including cells of the nucleus pulposus and annulus fibrosus).

Abstract translation: 描述了在细胞中诱导蛋白聚糖如聚集蛋白聚糖表达的方法。 描述了一种方法，其包括用分离的核酸转染细胞，所述分离的核酸包含可操作地连接于启动子的编码LIM矿化蛋白的核苷酸序列。 LIM矿化蛋白可以是rLMP，hLMP-1，hLMP-1或hLMP-3。 转染可以通过直接注射病毒或裸DNA或通过非病毒载体如质粒在体内或体内完成。 该方法可用于在骨细胞中诱导蛋白聚糖合成或刺激能够产生蛋白聚糖和/或胶原的细胞（例如包括髓核细胞和纤维环的椎间盘细胞）中的蛋白聚糖和/或胶原产生。

公开(公告)号：US20080166755A9

公开(公告)日：2008-07-10

申请号：US10806915

申请日：2004-03-23

Applicant: Frances Titus ,  Jeffrey Marx ,  Scott Boden ,  Sangwook Yoon ,  Susan Drapeau

Inventor： Frances Titus ,  Jeffrey Marx ,  Scott Boden ,  Sangwook Yoon ,  Susan Drapeau

IPC: C12P21/02 ,  C07K14/475 ,  C07H21/04

CPC classification number: C07K14/005 ,  A61K38/00 ,  C07K14/43581 ,  C07K14/4702 ,  C07K14/475 ,  C07K14/495 ,  C07K14/51 ,  C07K2319/00 ,  C12N2740/16322

Abstract: The invention provides fusion polypeptides comprising protein transduction domains and osteoinductive polypeptides, as well as methods of using such polypeptides to induce osteogenesis and to promote proteoglycan synthesis. The invention also provides osteoinductive peptides which have demonstrated the ability to induce bone formation in vivo.

Abstract translation: 本发明提供了包含蛋白转导结构域和骨诱导多肽的融合多肽，以及使用这些多肽诱导成骨和促进蛋白多糖合成的方法。 本发明还提供已经证明在体内诱导骨形成的能力的骨诱导肽。

公开(公告)号：US20070009493A1

公开(公告)日：2007-01-11

申请号：US11443387

申请日：2006-05-30

Applicant: Susan Drapeau ,  Sangwook Yoon

Inventor： Susan Drapeau ,  Sangwook Yoon

IPC: A61K35/14 ,  C12N5/08

CPC classification number: C12N5/0663 ,  A61K35/28 ,  A61K38/1875 ,  A61K38/57 ,  A61K48/005 ,  C12N2501/998 ,  C12N2502/11

Abstract: The invention provides a method of treatment joint and disk disease comprising administering to the subject in need thereof a novel composition comprising a substantially purified plurality of cells enhanced with at least one bioactive factor capable of causing at least a portion of the plurality of cells to express an increased amount of at least one chondrogenic marker.

Abstract translation: 本发明提供了一种治疗关节和椎间盘疾病的方法，包括向有需要的受试者施用新的组合物，其包含基本上纯化的多个细胞，所述多个细胞用至少一种能使至少一部分细胞表达的生物活性因子增强 增加量的至少一种软骨形成标记物。