公开(公告)号：US20110033836A1

公开(公告)日：2011-02-10

申请号：US12635539

申请日：2009-12-10

Applicant: DOUGLAS RICHMAN ,  MARY T. WRIN ,  SUSAN LITTLE ,  CHRISTOS J. PETROPOULOS ,  NEIL T. PARKIN ,  JEANNETTE WHITCOMB ,  WEI HUANG

Inventor： DOUGLAS RICHMAN ,  MARY T. WRIN ,  SUSAN LITTLE ,  CHRISTOS J. PETROPOULOS ,  NEIL T. PARKIN ,  JEANNETTE WHITCOMB ,  WEI HUANG

IPC: C12Q1/70

CPC classification number: G01N33/56988 ,  C07K14/005 ,  C07K14/16 ,  C07K14/162 ,  C07K16/08 ,  C07K16/10 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/56983 ,  G01N2333/16

Abstract: The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

Abstract translation: 本发明提供了鉴定化合物是否抑制病毒进入细胞的方法，该方法包括：（a）从病毒感染的患者获得编码病毒包膜蛋白的核酸; （b）共转染入第一细胞（i）步骤（a）的核酸，和（ii）缺乏编码包膜蛋白的核酸的病毒表达载体，其包含指示核酸，其产生 使得第一细胞产生包含由患者获得的核酸编码的包膜蛋白的病毒颗粒; （c）在化合物存在下使步骤（b）中产生的病毒颗粒与第二细胞接触，其中第二细胞表达病毒结合的细胞表面受体; （d）测量由第二细胞产生的信号量以确定病毒颗粒的感染性; 和（e）将步骤（d）中测量的信号量与在不存在化合物时产生的信号量进行比较，其中在化合物存在下测量的信号量减少表明化合物抑制病毒进入 进入第二个细胞。

公开(公告)号：US20050214743A1

公开(公告)日：2005-09-29

申请号：US10504921

申请日：2003-02-14

Applicant: Douglas Richman ,  Mary Wrin ,  Susan Little ,  Christos Petropoulos ,  Neil Parkin ,  Jeannette Whitcomb ,  Wei Huang

Inventor： Douglas Richman ,  Mary Wrin ,  Susan Little ,  Christos Petropoulos ,  Neil Parkin ,  Jeannette Whitcomb ,  Wei Huang

IPC: C12N15/86 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: G01N33/56988 ,  C07K14/005 ,  C07K14/16 ,  C07K14/162 ,  C07K16/08 ,  C07K16/10 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/56983 ,  G01N2333/16

Abstract: The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic add of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

Abstract translation: 本发明提供了鉴定化合物是否抑制病毒进入细胞的方法，该方法包括：（a）从病毒感染的患者获得编码病毒包膜蛋白的核酸; （b）共转染入第一细胞（i）步骤（a）的核酸添加，和（ii）缺乏编码包膜蛋白的核酸的病毒表达载体，其包含指示核酸，其产生 使得第一细胞产生包含由患者获得的核酸编码的包膜蛋白的病毒颗粒; （c）在化合物存在下使步骤（b）中产生的病毒颗粒与第二细胞接触，其中第二细胞表达病毒结合的细胞表面受体; （d）测量由第二细胞产生的信号量以确定病毒颗粒的感染性; 和（e）将步骤（d）中测量的信号量与在不存在化合物时产生的信号量进行比较，其中在化合物存在下测量的信号量减少表明化合物抑制病毒进入 进入第二个细胞。

公开(公告)号：US09175355B2

公开(公告)日：2015-11-03

申请号：US12635539

申请日：2009-12-10

Applicant: Douglas Richman ,  Mary T. Wrin ,  Susan Little ,  Christos J. Petropoulos ,  Neil T. Parkin ,  Jeannette Whitcomb ,  Wei Huang

Inventor： Douglas Richman ,  Mary T. Wrin ,  Susan Little ,  Christos J. Petropoulos ,  Neil T. Parkin ,  Jeannette Whitcomb ,  Wei Huang

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/569 ,  C07K14/16 ,  C07K16/08 ,  C07K14/005 ,  C07K16/10

CPC classification number: G01N33/56988 ,  C07K14/005 ,  C07K14/16 ,  C07K14/162 ,  C07K16/08 ,  C07K16/10 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/56983 ,  G01N2333/16

Abstract: The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

Abstract translation: 本发明提供了鉴定化合物是否抑制病毒进入细胞的方法，该方法包括：（a）从病毒感染的患者获得编码病毒包膜蛋白的核酸; （b）共转染入第一细胞（i）步骤（a）的核酸，和（ii）缺乏编码包膜蛋白的核酸的病毒表达载体，其包含指示核酸，其产生 使得第一细胞产生包含由患者获得的核酸编码的包膜蛋白的病毒颗粒; （c）在化合物存在下使步骤（b）中产生的病毒颗粒与第二细胞接触，其中第二细胞表达病毒结合的细胞表面受体; （d）测量由第二细胞产生的信号量以确定病毒颗粒的感染性; 和（e）将步骤（d）中测量的信号量与在不存在化合物时产生的信号量进行比较，其中在化合物存在下测量的信号量减少表明化合物抑制病毒进入 进入第二个细胞。

公开(公告)号：US07247439B1

公开(公告)日：2007-07-24

申请号：US10077027

申请日：2002-02-15

Applicant: Douglas Richman ,  Mary T. Wrin ,  Susan Little ,  Christos J. Petropoulos ,  Neil T. Parkin ,  Jeannette M. Whitcomb ,  Wei Huang

Inventor： Douglas Richman ,  Mary T. Wrin ,  Susan Little ,  Christos J. Petropoulos ,  Neil T. Parkin ,  Jeannette M. Whitcomb ,  Wei Huang

IPC: G01N33/53

CPC classification number: G01N33/56988 ,  C07K14/005 ,  C07K14/16 ,  C07K14/162 ,  C07K16/08 ,  C07K16/10 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/56983 ,  G01N2333/16

Abstract: The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

Abstract translation: 本发明提供了一种用于鉴定化合物是否抑制病毒进入细胞的方法，其包括：（a）从感染病毒的患者获得编码病毒包膜蛋白的核酸; （b）共转染入第一细胞（i）步骤（a）的核酸，和（ii）缺乏编码包膜蛋白的核酸的病毒表达载体，其包含指示核酸，其产生 使得第一细胞产生包含由患者获得的核酸编码的包膜蛋白的病毒颗粒; （c）在化合物存在下使步骤（b）中产生的病毒颗粒与第二细胞接触，其中第二细胞表达病毒结合的细胞表面受体; （d）测量由第二细胞产生的信号量以确定病毒颗粒的感染性; 和（e）将步骤（d）中测量的信号量与在不存在化合物时产生的信号量进行比较，其中在化合物存在下测量的信号量减少表明该化合物抑制病毒进入 进入第二个细胞。