公开(公告)号：US20240209016A1

公开(公告)日：2024-06-27

申请号：US18555180

申请日：2022-04-11

Applicant: STEALTH LABELS BIOTECH AB

Inventor： Elin ESBJÖRNER WINTERS ,  Marcus WILHELMSSON ,  Pauline PFEIFFER ,  Audrey GALLUD ,  Jesper NILSSON

IPC: C07H19/14 ,  C12N15/10

CPC classification number: C07H19/14 ,  C12N15/10

Abstract: This specification discloses novel phosphate compounds of formula (I) useful for labelling RNA in-cellulo. The phosphates are spontaneously taken up by cells and may be used as substrates for RNA synthesis once across the cell membrane. This technology therefore offers a general route to understanding the biological behaviour of RNA of interest, including RNA based drugs.

公开(公告)号：US20230219995A1

公开(公告)日：2023-07-13

申请号：US18001805

申请日：2021-06-24

Applicant: STEALTH LABELS BIOTECH AB

Inventor： Marcus WILHELMSSON ,  Elin ESBJÖRNER ,  Tom BALADI

IPC: C07H19/24 ,  C07H1/00 ,  C07H21/02 ,  G01N33/58

CPC classification number: C07H19/24 ,  C07H1/00 ,  C07H21/02 ,  G01N33/582 ,  C12Q1/6809

Abstract: This specification discloses a novel methodology for labelling RNA via enzymatic incorporation of a minimally perturbing fluorescent tricyclic cytosine analogue. This analogue is shown to be 100% incorporated in example transcripts and is fully compatible with both in vitro and in cell transcription. Spectroscopic characterization shows that the incorporation rate of the cytosine analogue is on par with its natural counterpart. Using live cell imaging and flow cytometry, labelled mRNAs are efficiently and correctly translated upon transfection into living cells and cell-free systems. The spectral properties of the modified transcripts and their correct translation product allow for their straightforward and simultaneous visualization. This technology therefore offers a general route to understanding the biological behaviour of RNA of interest, including RNA based drugs. The fluorescent tricyclic cytosine analogue has formula (I):