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    Identifying and quantifying small RNAs
    1.
    发明申请
    Identifying and quantifying small RNAs 审中-公开
    识别和定量小RNA

    公开(公告)号：US20090220969A1

    公开(公告)日：2009-09-03

    申请号：US12231525

    申请日：2008-09-03

    Applicant: Vincent Chiang ,  Rui Shi

    Inventor： Vincent Chiang ,  Rui Shi

    IPC: C12Q1/68 ,  C12P19/34 ,  C12N9/12

    CPC classification number: C12Q1/6853 ,  C12Q2525/173 ,  C12Q2521/119 ,  C12Q2521/107

    Abstract: One-step RT-PCR methods, compositions and kits for the detection and quantification of small RNAs in a sample are disclosed. The one-step RT-PCR approach involves polyadenylation of a small RNA followed by reverse transcription with a first primer containing a poly(T) sequence and at least two 3′ nucleotides complementary to the 3′ terminal end nucleotides of the small RNA, to produce a cDNA. This may be followed by PCR amplification using the same first primer as the revere primer and a second, forward primer in which a portion of its sequence is complementary to the 3′ terminal end of the cDNA. This may be then followed by detection and/or quantification of the amplified product.

    Abstract translation: 公开了用于检测和定量样品中的小RNA的一步RT-PCR方法，组合物和试剂盒。 一步RT-PCR方法涉及小RNA的多聚腺苷酸化，然后用含有poly（T）序列的第一引物和与小RNA的3'末端核苷酸互补的至少两个3'核苷酸进行逆转录， 产生cDNA。 然后可以使用与反向引物相同的第一引物进行PCR扩增，第二引物其序列的一部分与cDNA的3'末端互补。 然后可以检测和/或定量扩增产物。

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