Cheesemaking with recombinant aspartic protease
    3.
    发明授权
    Cheesemaking with recombinant aspartic protease 失效
    用重组天冬氨酸蛋白酶制作

    公开(公告)号:US5800849A

    公开(公告)日:1998-09-01

    申请号:US535237

    申请日:1995-12-29

    CPC classification number: A23C19/0326 C12N9/58

    Abstract: A process of producing cheese in improved yields, wherein a recombinant aspartic protease, derived from Rhizomucor miehei or Rhizomucor pusillus, is added to milk in sufficient amounts to effect clotting of the milk, after which the resulting curd is processed in a manner known per se for making cheese.

    Abstract translation: PCT No.PCT / DK94 / 00163 Sec。 371 1995年12月29日第 102(e)1995年12月29日PCT PCT 1994年4月22日PCT公布。 公开号WO94 / 24880 日期1994年11月10日以提高的产量生产干酪的方法,其中将来自根霉(Rhizomucor miehei)或根毛霉(Rhizomucor pusillus)的重组天冬氨酸蛋白酶以足够的量添加到乳中,以实现乳的凝结,之后将所得凝乳加工 本身已知的制作奶酪的方式。

    Method of producing non-bovine chymosin and use hereof
    4.
    发明申请
    Method of producing non-bovine chymosin and use hereof 审中-公开
    生产非牛凝乳酶的方法及其应用

    公开(公告)号:US20080226768A1

    公开(公告)日:2008-09-18

    申请号:US11898540

    申请日:2007-09-13

    CPC classification number: A23C19/043 C12N9/6483 C12Y304/23004

    Abstract: A method of recombinantly producing a non-bovine pre-prochymosin, prochymosin or chymosin derived from ruminant species including deer species, buffalo species, antelope species, giraffe species, ovine species and caprine species; Camelidae species such as Camelus dromedarius; porcine species; or Equidae species. The recombinant enzymes are used in milk coagulating compositions in cheese manufacturing based on cow's milk and milk from any animal species which are used in cheese manufacturing including camel's milk.

    Abstract translation: 一种重组产生源自鹿类,水牛种,羚羊种,长颈鹿物种,羊种和山羊种的反刍动物物种的非牛前凝乳酶原,凝乳酶原或凝乳酶的方法; 骆驼科(Camelidae)种如骆驼属骆驼属(Camelus dromedarius); 猪种; 或Equidae种。 重组酶用于奶酪制造中的牛奶凝固组合物,其基于来自用于奶酪制造的任何动物物种的牛奶和牛奶,包括骆驼乳。

    Process for making cheese
    5.
    发明授权
    Process for making cheese 失效
    制作奶酪的过程

    公开(公告)号:US06258390B1

    公开(公告)日:2001-07-10

    申请号:US08990884

    申请日:1997-12-15

    Applicant: Peter Budtz

    Inventor: Peter Budtz

    CPC classification number: C12Y203/02013 A23C19/0328 A23C19/043

    Abstract: A process for making cheese including: a) adding to cheesemilk a transglutaminase, incubating for a suitable period, b) incubating with a rennet so as to cause clotting, and c) separating whey from the coagulate, and d) processing the coagulate into cheese. Cheese products produced by said process are contemplated and to the use of transglutaminase for maintaining proteins in the cheese material during a conventional cheese-making process.

    Abstract translation: 一种制备奶酪的方法,包括:a)将酪氨酸酶加入转谷氨酰胺酶,孵育适合的时间,b)与凝乳酶一起孵育以引起凝血,和c)从凝血酶中分离乳清,和d)将凝固物加工成奶酪 。 考虑到由所述方法生产的奶酪产品以及在常规奶酪制备过程中使用转谷氨酰胺酶来保持酪乳酪材料中的蛋白质。

    Method for hydrolyzing proteins with gluyasp specific protease
    6.
    发明授权
    Method for hydrolyzing proteins with gluyasp specific protease 失效
    用gluyasp特异性蛋白酶水解蛋白质的方法

    公开(公告)号:US5866357A

    公开(公告)日:1999-02-02

    申请号:US927661

    申请日:1992-08-31

    CPC classification number: A23J3/34

    Abstract: A method is disclosed for obtaining limited specific hydrolysis of proteins using a Glu/Asp specific protease to produce peptides with C-terminal Glu or Asp. The protease is obtained from Bacillus licheniformis, has a mass of about 23,600, is inhibited by diisopropyl fluoride but not by phenylmethane sulfonylfluoride, and has a pH of maximal activity between 6.5 and 10.0. The proteins are treated with the protease at neutral pH and the reaction terminated by raising the temperature above 70.degree. C. or lowering the pH below 5.0 to obtain the hydrolysate. The process may include a second protease which is specific for Arg/Lys, and produces peptides with C-terminal Arg or Lys.

    Abstract translation: PCT No.PCT / DK91 / 00069 Sec。 371日期:1992年8月31日 102(e)日期1992年8月31日PCT 1991年3月8日PCT公布。 公开号WO91 / 13554 1991年9月19日公开了一种用于使用Glu / Asp特异性蛋白酶获得有限的蛋白质特异性水解以产生具有C端Glu或Asp的肽的方法。 蛋白酶从地衣芽孢杆菌获得,质量约为23,600,被二异丙基氟化物抑制,但不被苯基甲烷磺酰氟抑制,并且具有6.5至10.0之间最大活性的pH。 蛋白质在中性pH下用蛋白酶处理,并且通过将温度升高至70℃以上或将pH降低至5.0以终止反应,得到水解产物。 该方法可以包括对Arg / Lys特异的第二种蛋白酶,并产生具有C末端Arg或Lys的肽。

    Protein preparations
    10.
    发明授权
    Protein preparations 失效
    蛋白质制剂

    公开(公告)号:US5523237A

    公开(公告)日:1996-06-04

    申请号:US90048

    申请日:1993-07-16

    CPC classification number: A23J3/34

    Abstract: A plastein material is made by reversing the normal hydrolytic activity of a serine protease. The protease produces a plastein material by acting on a proteinaceous substrate. The substrate is preferably whey, casein or soy protein.

    Abstract translation: PCT No.PCT / DK92 / 00036 Sec。 371日期:1993年7月16日 102(e)日期1993年7月16日PCT提交1992年2月6日PCT公布。 No.W092 / 13964 PCT出版物 日期:1992年8月20日。通过逆转丝氨酸蛋白酶的正常水解活性来制备平滑素材料。 蛋白酶通过作用在蛋白质底物上而产生Plastein材料。 底物优选为乳清,酪蛋白或大豆蛋白。

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