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1.发明授权Integrated microfluidic component for purifying analyte molecules and purification method 失效用于纯化分析物分子的集成微流体组分和纯化方法公开(公告)号：US08431339B2
公开(公告)日：2013-04-30
申请号：US12447305
申请日：2007-10-26
申请人： Paul Vulto , Gerald Urban
发明人： Paul Vulto , Gerald Urban
CPC分类号： C12N15/101 , B01L3/502753 , B01L2200/0631 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2400/0421 , G01N27/44791
摘要： The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section.
摘要翻译： 本发明涉及一种用于纯化分析物分子的方法，特别涉及一种分离部分用于分离分析物和样品其他组分的这类成分，其中提供至少一个样品室 用于接收含有分析物分子的样品和用于接收纯化的分析物分子的至少一个收集室。 根据本发明，微流体组分具有至少一个用于检测纯化的分析物分子的存在和/或浓度的集成受体装置。 根据本发明的一个有利的发展，分离部由电泳凝胶过滤部形成。
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公开(公告)号：US09771553B2
公开(公告)日：2017-09-26
申请号：US14003606
申请日：2012-03-08
CPC分类号： C12M27/18 , B01F13/0083 , B01L3/502746 , B01L3/56 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2300/0816 , B01L2300/0851 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/0688 , B01L2400/086 , B01L2400/088 , C12M23/16 , C12M23/34 , C12M29/10 , C12M35/08 , Y10T137/0318
摘要： Apparatus for processing liquids or liquid-based substances includes a plurality of volumes at least two of which are defined at least in part by one or more phaseguides inside the volume and/or in a conduit connected thereto for controlling aliquoting of one or more liquids or liquid-based substances inside the volume. Each volume has an upstream and downstream side with respect to meniscus advancement direction via which it may be filled with or emptied of one or more liquids or liquid-based substances. The apparatus also includes at least one common upstream-side conduit connected to supply a liquid or liquid-based substance via a plurality of the inlet or extraction conduits, a plurality of the phaseguides exhibiting a predetermined level of stability and one or more of the phaseguides exhibiting a predetermined different stability compared with the stability of at least one of the other phaseguides whereby to control the preference order in which the volumes fill and/or empty. The stability is determined by the value and radius of an acute angle along a said phaseguide at the downstream side of the phaseguide.
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公开(公告)号：US20120097272A1
公开(公告)日：2012-04-26
申请号：US13147070
申请日：2010-01-29
申请人： Paul Vulto , Gerald Urban , Susann Podszun
发明人： Paul Vulto , Gerald Urban , Susann Podszun
IPC分类号： F15D1/00
CPC分类号： B01L3/502707 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L3/502784 , B01L2200/0621 , B01L2200/0642 , B01L2300/0816 , B01L2300/0851 , B01L2300/087 , B01L2300/0874 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/0605 , B01L2400/0688 , B01L2400/082 , B01L2400/086 , B01L2400/088 , Y10T137/8593
摘要： The present invention relates to phaseguide patterns for use in fluid systems such as channels, chambers, and flow through cells. In order to effectively control filling and/or emptying of fluidic chambers and channels, techniques for a controlled overflowing of phaseguides are proposed. In addition, techniques of confined liquid patterning in a larger fluidic structure, including approaches for patterning overflow structures and the specific shape of phaseguides, are provided. The invention also proposes techniques to effectively rotate the advancement of a liquid/air meniscus over a certain angle. In particular, a phaseguide pattern for guiding a flow of a liquid contained within a compartment is provided, wherein an overflow of the phaseguide by a moving liquid phase is controlled by a local change in capillary force along the phaseguide, wherein said overflow by the liquid over the phaseguide is provoked at the position of the local change in capillary force.
摘要翻译： 本发明涉及用于流体系统（例如通道，室和流通池）中的相位导图。 为了有效地控制流体室和通道的填充和/或排空，提出了用于受控溢流的相位导引的技术。 此外，还提供了在更大的流体结构中的限制液体图案化技术，包括图案化溢出结构的方法和相位导体的具体形状。 本发明还提出了在一定角度上有效地旋转液体/空气弯液面的推进的技术。 特别地，提供了用于引导容纳在隔间内的液体的流动的相位指示图案，其中通过移动液相的相位导向器的溢流通过沿着相位导管的毛细管力的局部变化来控制，其中所述液体溢出 在该位置上引发了相位导致毛细血管力变化的位置。
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公开(公告)号：US10450542B2
公开(公告)日：2019-10-22
申请号：US14003620
申请日：2012-03-08
申请人： Paul Vulto , Sebastiaan Johannes Trietsch , Heiko Jan van der Linden , Adrianus Theodorus Joore , Thomas Hankemeier
发明人： Paul Vulto , Sebastiaan Johannes Trietsch , Heiko Jan van der Linden , Adrianus Theodorus Joore , Thomas Hankemeier
摘要： Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.
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5.发明申请公开(公告)号：US20130340883A1
公开(公告)日：2013-12-26
申请号：US14003606
申请日：2012-03-08
IPC分类号： B01L3/00
CPC分类号： C12M27/18 , B01F13/0083 , B01L3/502746 , B01L3/56 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2300/0816 , B01L2300/0851 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/0688 , B01L2400/086 , B01L2400/088 , C12M23/16 , C12M23/34 , C12M29/10 , C12M35/08 , Y10T137/0318
摘要： Apparatus for processing liquids or liquid-based substances includes a plurality of volumes at least two of which are defined at least in part by one or more phaseguides inside the volume and/or in a conduit connected thereto for controlling aliquoting of one or more liquids or liquid-based substances inside the volume. Each volume has an upstream and downstream side with respect to meniscus advancement direction via which it may be filled with or emptied of one or more liquids or liquid-based substances. The apparatus also includes at least one common upstream-side conduit connected to supply a liquid or liquid-based substance via a plurality of the inlet or extraction conduits, a plurality of the phaseguides exhibiting a predetermined level of stability and one or more of the phaseguides exhibiting a predetermined different stability compared with the stability of at least one of the other phaseguides whereby to control the preference order in which the volumes fill and/or empty. The stability is determined by the value and radius of an acute angle along a said phaseguide at the downstream side of the phaseguide.
摘要翻译： 用于处理液体或液体物质的装置包括多个体积，其中至少两个体积至少部分地由体积内的一个或多个相导体和/或与其连接的导管中限定，用于控制一种或多种液体的等分或 体内液体物质。 每个体积具有相对于弯液面前进方向的上游侧和下游侧，通过该方向可以填充或排空一种或多种液体或液体物质。 该设备还包括至少一个公共上游侧管道，其连接用于经由多个入口或抽取管道供应液体或液体物质，多个相位导线呈现预定水平的稳定性，以及一个或多个相位导数 与至少一个其它相导体的稳定性相比具有预定的不同稳定性，从而控制体积填充和/或空的倾向顺序。 稳定性由沿着相导向器下游侧的所述相位导轨的锐角的值和半径确定。
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公开(公告)号：US09174215B2
公开(公告)日：2015-11-03
申请号：US13147070
申请日：2010-01-29
申请人： Paul Vulto , Gerald Urban , Susann Podszun
发明人： Paul Vulto , Gerald Urban , Susann Podszun
CPC分类号： B01L3/502707 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L3/502784 , B01L2200/0621 , B01L2200/0642 , B01L2300/0816 , B01L2300/0851 , B01L2300/087 , B01L2300/0874 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/0605 , B01L2400/0688 , B01L2400/082 , B01L2400/086 , B01L2400/088 , Y10T137/8593
摘要： The present invention relates to phaseguide patterns for use in fluid systems such as channels, chambers, and flow through cells. In order to effectively control filling and/or emptying of fluidic chambers and channels, techniques for a controlled overflowing of phaseguides are proposed. In addition, techniques of confined liquid patterning in a larger fluidic structure, including approaches for patterning overflow structures and the specific shape of phaseguides, are provided. The invention also proposes techniques to effectively rotate the advancement of a liquid/air meniscus over a certain angle. In particular, a phaseguide pattern for guiding a flow of a liquid contained within a compartment is provided, wherein an overflow of the phaseguide by a moving liquid phase is controlled by a local change in capillary force along the phaseguide, wherein said overflow by the liquid over the phaseguide is provoked at the position of the local change in capillary force.
摘要翻译： 本发明涉及用于流体系统（例如通道，室和流通池）中的相位导图。 为了有效地控制流体室和通道的填充和/或排空，提出了用于受控溢流的相位导引的技术。 此外，还提供了在更大的流体结构中的限制液体图案化技术，包括图案化溢出结构的方法和相位导体的具体形状。 本发明还提出了在一定角度上有效地旋转液体/空气弯液面的推进的技术。 特别地，提供了用于引导容纳在隔间内的液体的流动的相位指示图案，其中通过移动液相的相位导向器的溢流通过沿着相位导管的毛细管力的局部变化来控制，其中所述液体溢出 在该位置上引发了相位导致毛细血管力变化的位置。
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7.发明申请公开(公告)号：US20140065597A1
公开(公告)日：2014-03-06
申请号：US14003620
申请日：2012-03-08
申请人： Paul Vulto , Sebastiaan Johannes Trietsch , Heiko Jan Van der linden , Adrianus Theodorus Joore , Thomas Hankemeier
发明人： Paul Vulto , Sebastiaan Johannes Trietsch , Heiko Jan Van der linden , Adrianus Theodorus Joore , Thomas Hankemeier
IPC分类号： C12M1/00
CPC分类号： C12M27/18 , B01F13/0083 , B01L3/502746 , B01L3/56 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2300/0816 , B01L2300/0851 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/0688 , B01L2400/086 , B01L2400/088 , C12M23/16 , C12M23/34 , C12M29/10 , C12M35/08 , Y10T137/0318
摘要： Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.
摘要翻译： 用于处理基于生命的有机颗粒的装置，包括选自包括细胞，细胞球体，组织，真核生物，微生物，器官或胚胎的颗粒的颗粒，包括中空体积（10），其中（a）至少内部分为 第一（14），第二（16）和第三（17）子体积，形成在容积内形成的至少两个相位导体（12,13），（b）包括相对上游和相对下游的部分， 弯液面或大量液体在体积（10）中的移动。 该装置包括至少第一，第二和第三流体导管（19,21,22），其被连接以允许容积（10）的上游外部与相应的所述子体积（14,16,17）之间的流体连通。 以及至少一个另外的导管（24），其连接以允许容积（10）的下游外部与所述子体积之间的流体连通。 第一子体积（14）包含一种或多种在凝胶或凝胶状物质中或由凝胶状物质支持的基于生命的颗粒; 并且所述第二子体积（16）与所述第一子体积连通以允许物质在所述第一和第二子体积（14,16）之间运输并且包含至少一种凝胶或凝胶状物质。
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8.发明申请Integrated Microfluidic Component for Purifying Analyte Molecules and Purification Method 失效用于纯化分析物分子的集成微流体组分和纯化方法公开(公告)号：US20100084270A1
公开(公告)日：2010-04-08
申请号：US12447305
申请日：2007-10-26
申请人： Paul Vulto , Gerald Urban
发明人： Paul Vulto , Gerald Urban
CPC分类号： C12N15/101 , B01L3/502753 , B01L2200/0631 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2400/0421 , G01N27/44791
摘要： The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section.
摘要翻译： 本发明涉及一种用于纯化分析物分子的方法，特别涉及一种分离部分用于分离分析物和样品其他组分的这类成分，其中提供至少一个样品室 用于接收含有分析物分子的样品和用于接收纯化的分析物分子的至少一个收集室。 根据本发明，微流体组分具有至少一个用于检测纯化的分析物分子的存在和/或浓度的集成受体装置。 根据本发明的一个有利的发展，分离部由电泳凝胶过滤部形成。
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