公开(公告)号：US4560534A

公开(公告)日：1985-12-24

申请号：US548152

申请日：1983-11-02

申请人： Wei-jen Kung ,  Paul O. Vogelhut

发明人： Wei-jen Kung ,  Paul O. Vogelhut

IPC分类号： G01N33/50 ,  C12Q1/00 ,  G01N27/02 ,  G01N27/12 ,  G01N33/487 ,  G01N27/00

CPC分类号： C12Q1/001 ,  C12Q1/006

摘要： Polymer-catalyst transducers are used for detecting one or more constituents in a sample. Changes in conductivity of the polymer-catalyst transducers as a result of electron density changes are measured to provide an indication of a particular constituent present in the sample. Such conductivity changes can be measured using a conductivity meter. The polymer-catalyst transducers are particularly useful for the determination of glucose in whole blood.

摘要翻译： 聚合物 - 催化剂换能器用于检测样品中的一种或多种组分。 测量作为电子密度变化的结果的聚合物 - 催化剂换能器的电导率的变化以提供样品中存在的特定成分的指示。 可以使用电导率仪测量这种电导率变化。 聚合物 - 催化剂换能器特别可用于测定全血中的葡萄糖。

公开(公告)号：US4396579A

公开(公告)日：1983-08-02

申请号：US290656

申请日：1981-08-06

申请人： Hartmut R. Schroeder ,  Paul O. Vogelhut

发明人： Hartmut R. Schroeder ,  Paul O. Vogelhut

IPC分类号： G01N21/76 ,  G01N33/58 ,  C12M1/20

CPC分类号： G01N33/582 ,  G01N21/76 ,  Y10S435/835 ,  Y10T436/206664

摘要： A luminescence detection device for quantitatively detecting an analyte in a liquid sample, which device comprises (1) a reaction compartment having, along a primary axis, opposite end portions, a first of which is for introduction of fluid reagents and sample into the compartment and the other of which forms a light transmissive aperture of predetermined size, the compartment being suitable to hold a composition which luminesces in response to contact with analyte-containing sample; (2) closure means in said first end portion for admitting a cannula, whereby fluid is introduced into said compartment, and for closing the reaction compartment; (3) a photoresponsive imaging layer; (4) means for associating the photoresponsive imaging layer and the reaction compartment such that the photoresponsive imaging layer is positioned preferably substantially perpendicular to the primary axis of the reaction compartment, at a predetermined distance from the end portion forming the aperture so as to be exposed to light emanating therefrom; and (5) means for preventing exposure of the photoresponsive imaging layer to ambient light.

摘要翻译： 一种用于定量检测液体样品中的分析物的发光检测装置，该装置包括（1）反应室，其沿主轴线具有相对的端部，第一个反应室用于将流体试剂和样品引入隔室， 另一个形成预定尺寸的透光孔，该隔室适于容纳响应于与含分析物的样品接触而发光的组合物; （2）在所述第一端部中用于允许插管的封闭装置，由此将流体引入所述隔间并关闭所述反应室; （3）光响应成像层; （4）用于使光响应成像层和反应室相关联的装置，使得光响应成像层优选地基本上垂直于反应室的主轴定位，距离形成孔的端部预定距离以暴露 从其发出的光; 和（5）用于防止光响应成像层暴露于环境光的装置。

公开(公告)号：US5210217A

公开(公告)日：1993-05-11

申请号：US335563

申请日：1989-04-10

申请人： James P. Albarella ,  J. Oakey Noell ,  Paul O. Vogelhut ,  Frederick E. Ward, deceased

发明人： James P. Albarella ,  J. Oakey Noell ,  Paul O. Vogelhut ,  Frederick E. Ward, deceased

IPC分类号： C07D409/04 ,  C07D409/14 ,  C07D521/00 ,  C08G61/12 ,  C12Q1/00 ,  C12Q1/54 ,  G01N33/532 ,  H01B1/12

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D409/04 ,  C07D409/14 ,  C08G61/123 ,  C12Q1/002 ,  C12Q1/54 ,  G01N33/532 ,  H01B1/127

摘要： Electrically conducting homo- and/or copolymers and/or tripolymers can be produced from novel monomers, such as a 3-substituted 2,5-di(2-thienyl)pyrrole. The polymers exhibit unexpectedly high stability and conductivities, and can be functionalized, such as with an enzyme, like glucose oxidase, or an ion-specific binding site, like a crown ether, or an antigen, without adversely affecting the conductivity of the polymer. The functionalized, conducting polymer can be used in a diagnostic device to determine the presence and concentration of a specific analyte in a liquid medium. For example, the presence and concentration of glucose is determined by measuring the conductivity change in the polymer caused by the vibrational excitation induced in the enzyme, glucose oxidase, from its reaction with the glucose and/or by measuring a secondary effect of the enzyme/substrate reaction, such as the change in the conductivity of the conducting polymer caused by the generation of hydrogen peroxide during the glucose-glucose oxidase reaction.

摘要翻译： 导电的均聚物和/或共聚物和/或三聚物可以由新型单体如3-取代的2,5-二（2-噻吩基）吡咯制备。 聚合物表现出意想不到的高稳定性和电导率，并且可以如酶，如葡萄糖氧化酶，或离子特异性结合位点，如冠醚或抗原而被官能化，而不会不利地影响聚合物的导电性。 官能化的导电聚合物可用于诊断装置中以确定液体介质中特定分析物的存在和浓度。 例如，葡萄糖的存在和浓度通过测量由酶，葡萄糖氧化酶，其与葡萄糖的反应引起的振动激发引起的聚合物中的电导率变化和/或通过测量酶/ 底物反应，例如在葡萄糖 - 葡萄糖氧化酶反应期间由过氧化氢的产生引起的导电聚合物的导电性的变化。

公开(公告)号：US4886625A

公开(公告)日：1989-12-12

申请号：US114011

申请日：1987-10-29

申请人： James P. Albarella ,  J. Oakey Noell ,  Paul O. Vogelhut ,  deceased, Frederick E. Ward

发明人： James P. Albarella ,  J. Oakey Noell ,  Paul O. Vogelhut ,  deceased, Frederick E. Ward

IPC分类号： C12Q1/26 ,  C07D333/40 ,  C07D409/04 ,  C07D409/14 ,  C07D419/14 ,  C07D421/04 ,  C07D421/14 ,  C07D521/00 ,  C08G61/10 ,  C08G61/12 ,  C12Q1/00 ,  C12Q1/54 ,  G01N33/532 ,  G01N33/543 ,  H01B1/12

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D409/04 ,  C07D409/14 ,  C08G61/123 ,  C12Q1/002 ,  C12Q1/54 ,  G01N33/532 ,  H01B1/127

摘要： Electrically conducting homo- and/or copolymers and/or tripolymers can be produced from novel monomers, such as a 3-substituted 2,5-di(2-thienyl)pyrrole. The polymers exhibit unexpectedly high stability and conductivities, and can be functionalized, such as with an enzyme, like glucose oxidase, or an ion-specific binding site, like a crown ether, or an antigen, without adversely affecting the conductivity of the polymer. The functionalized, conducting polymer can be used in a diagnostic device to determine the presence and concentration of a specific analyte in a liquid medium. For example, the presence and concentration of glucose is determined by measuring the conductivity change in the polymer caused by the vibrational excitation induced in the enzyme, glucose oxidase, from its reaction with the glucose and/or by measuring a secondary effect of the enzyme/substrate reaction, such as the change in the conductivity of the conducting polymer caused by the generation of hydrogen peroxide during the glucose-glucose oxidase reaction.

公开(公告)号：US4231754A

公开(公告)日：1980-11-04

申请号：US41682

申请日：1979-05-23

申请人： Paul O. Vogelhut

发明人： Paul O. Vogelhut

IPC分类号： C12Q1/66 ,  G01N21/76 ,  G01N31/22 ,  G01N33/52 ,  G01N33/50

CPC分类号： G01N21/76 ,  C12Q1/66 ,  G01N33/525 ,  Y10S435/805

摘要： A device, method of making the device and method for determining a constituent in a sample by production of luminescence are disclosed. More particularly, there is provided a test device for determining an analyte in a sample comprising unitary solid carrier means incorporated with a first reagent system responsive to the presence of said analyte to produce a reaction product and a second reagent system responsive to the presence of said reaction product to produce luminescence. The test device can further comprise a photoresponsive layer physically associated with said carrier means and responsive to light produced by the chemiluminescent system.

摘要翻译： 公开了一种制造该器件的方法，以及通过产生发光来确定样品中成分的方法。 更具体地，提供了一种用于确定样品中的分析物的测试装置，其包括结合有第一试剂系统的单一固体载体装置，其响应于所述分析物的存在而产生反应产物和第二试剂系统，所述第二试剂系统响应于所述 反应产物产生发光。 测试装置还可以包括物理上与所述载体装置相关联并响应于由化学发光系统产生的光的光响应层。