Chemical reagents and methods for detection and quantification of proteins in complex mixtures
    1.
    发明授权
    Chemical reagents and methods for detection and quantification of proteins in complex mixtures 有权
    用于复杂混合物中蛋白质的检测和定量的化学试剂和方法

    公开(公告)号:US07473535B2

    公开(公告)日:2009-01-06

    申请号:US10225447

    申请日:2002-08-20

    IPC分类号: G01N33/53 A61K38/00 C07K1/00

    摘要: The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.

    摘要翻译: 本发明提供了包含亲和标签,可检测部分,接头,同位素标签和反应性基团的试剂。 本发明还提供了使用本发明试剂的方法。 该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。 本发明另外提供了分离,鉴定和定量样品中的多肽的方法。 本发明还提供使用本发明的试剂诊断疾病的方法。