公开(公告)号：US08376258B2

公开(公告)日：2013-02-19

申请号：US12904955

申请日：2010-10-14

申请人： Takayoshi Ando ,  Teruhiko Koide ,  Masafumi Tsujimoto

发明人： Takayoshi Ando ,  Teruhiko Koide ,  Masafumi Tsujimoto

IPC分类号： B65H75/48

CPC分类号： B60R22/46 ,  B60R2022/468

摘要： A webbing take-up device enabling a reduction in size of an overload release mechanism and easy adjustment of an operation torque of the overload release mechanism is provided. In the overload release mechanism of this webbing take-up device, rotary force of a large diameter gear is transmitted to a small diameter gear via plural annular friction springs. The friction springs are disposed between an inner periphery face of a main body portion of the large diameter gear and a tubular portion of the small diameter gear, side by side along an axial direction of the two gears. Consequently, a radial direction enlargement of the large diameter gear and small diameter gear due to space for disposing the friction springs may be restrained. Moreover, the operation torque of the overload release mechanism may be adjusted stepwise by changing the number of the friction springs to be used, in accordance with requirements.

摘要翻译： 提供一种能够减小过载释放机构的尺寸并且容易调节过载释放机构的操作扭矩的织带卷取装置。 在这种织带卷取装置的过载释放机构中，大直径齿轮的旋转力通过多个环形摩擦弹簧传递到小直径齿轮。 摩擦弹簧沿着两个齿轮的轴向并排设置在大直径齿轮的主体部分的内周面和小直径齿轮的管状部分之间。 因此，可以抑制由于设置摩擦弹簧的空间而引起的大直径齿轮和小直径齿轮的径向扩大。 此外，可以根据要求通过改变要使用的摩擦弹簧的数量来逐步地调节过载释放机构的操作扭矩。

公开(公告)号：US06323017B1

公开(公告)日：2001-11-27

申请号：US09196222

申请日：1998-11-20

申请人： Hideki Adachi ,  Masafumi Tsujimoto ,  Hiroyuki Arai ,  Keizo Inoue

发明人： Hideki Adachi ,  Masafumi Tsujimoto ,  Hiroyuki Arai ,  Keizo Inoue

IPC分类号： C12N914

CPC分类号： C12N9/18

摘要： The present invention relates to an isolated human protein having platelet activating factor acetylhydrolase activity.

摘要翻译： 本发明涉及具有血小板活化因子乙酰水解酶活性的分离的人蛋白质。

公开(公告)号：US5880272A

公开(公告)日：1999-03-09

申请号：US961716

申请日：1997-10-31

申请人： Hideki Adachi ,  Masafumi Tsujimoto ,  Keizo Inoue ,  Hiroyuki Arai

发明人： Hideki Adachi ,  Masafumi Tsujimoto ,  Keizo Inoue ,  Hiroyuki Arai

IPC分类号： C12N9/16 ,  C12N9/18 ,  C12N15/09 ,  C12N15/53 ,  C12N9/20 ,  C12N15/55

CPC分类号： C12N9/18

摘要： An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.

摘要翻译： 提供氧化磷脂降解酶，在生物体的氧应激预防机制中起重要作用。 该酶水解1-酰基-2-巯基脂肪酸酰基-3-磷脂酰胆碱作为其2-酯键的底物，形成1-酰基-2-溶血-3-磷脂酰胆碱。 该酶的活性由4mM氯化钙稍微增强。 酶的分子量为95 +/- 5kDa（通过凝胶过滤）。 该酶由三个亚基组成，其分子量分别通过SDS-聚丙烯酰胺电泳发现为29kDa，30kDa和45kDa。 还提供了编码酶的基因。 该基因对通过遗传工程合成酶非常重要。

公开(公告)号：US5972886A

公开(公告)日：1999-10-26

申请号：US611977

申请日：1996-03-06

申请人： Masafumi Tsujimoto ,  Fuyuki Iwasa ,  Nobuo Tsuruoka ,  Hiroshi Nakazato ,  Kenju Miura ,  Nobuhiro Ishida ,  Tatsuya Kurihara ,  Kozo Yamaichi ,  Nozomi Yamaguchi

发明人： Masafumi Tsujimoto ,  Fuyuki Iwasa ,  Nobuo Tsuruoka ,  Hiroshi Nakazato ,  Kenju Miura ,  Nobuhiro Ishida ,  Tatsuya Kurihara ,  Kozo Yamaichi ,  Nozomi Yamaguchi

IPC分类号： A61K38/00 ,  A61K38/19 ,  A61P7/00 ,  C07K1/20 ,  C07K14/00 ,  C07K14/47 ,  C07K14/475 ,  C07K16/22 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/18 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91 ,  C07K14/52 ,  A61K38/18

CPC分类号： C07K14/475 ,  A61K38/00

摘要： A novel megakaryocyte differentiation factor, for example, consisting essentially of SEQ ID NO: 30; DNA coding for the megakaryocyte differentiation factor, an expression vector comprising the DNA, a host transformed with the expression vector, and a process for production of the megakaryocyte differentiation factor using the host.The megakaryocyte differentiation factor accelerates differentiation of megakaryocytes in the presence of IL-3, and acts as a thrombopoietin, and therefore an effective medicament to various diseases involving a decrease in platelete.

公开(公告)号：US5831030A

公开(公告)日：1998-11-03

申请号：US472659

申请日：1995-06-07

申请人： Masafumi Tsujimoto ,  Fuyuki Iwasa ,  Nobuo Tsuruoka ,  Hiroshi Nakazato ,  Kenju Miura ,  Nobuhiro Ishida ,  Tatsuya Kurihara ,  Kozo Yamaichi ,  Nozomi Yamaguchi

发明人： Masafumi Tsujimoto ,  Fuyuki Iwasa ,  Nobuo Tsuruoka ,  Hiroshi Nakazato ,  Kenju Miura ,  Nobuhiro Ishida ,  Tatsuya Kurihara ,  Kozo Yamaichi ,  Nozomi Yamaguchi

IPC分类号： A61K38/00 ,  A61K38/19 ,  A61P7/00 ,  C07K1/20 ,  C07K14/00 ,  C07K14/47 ,  C07K14/475 ,  C07K16/22 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/18 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91

CPC分类号： C07K14/475 ,  A61K38/00

摘要： The present invention provides novel megakaryocyte differentiation factors, which are useful as therapeutic agents due to their accelerating effects on the differentiation of megakaryocytes and their thrombopoietic effects. The megakaryocyte differentiation factors of the present invention include the megakaryocyte differentiation factor having the amino acid sequence shown in SEQ ID NO: 34. The present invention also provides DNA coding for the megakaryocyte differentiation factors of the present invention, expression vectors containing the DNA, hosts transformed with the expression vectors, and processes for production of the megakaryocyte differentiation factors using transformed hosts. The present invention further provides specific antibodies to the megakaryocyte differentiation factors of the present invention.

摘要翻译： 本发明提供了新的巨核细胞分化因子，由于它们对巨核细胞的分化及其血小板生成作用的加速作用，它们可用作治疗剂。 本发明的巨核细胞分化因子包括具有SEQ ID NO：34所示的氨基酸序列的巨核细胞分化因子。本发明还提供编码本发明的巨核细胞分化因子的DNA，含有该DNA的表达载体，宿主 用表达载体转化，以及使用转化宿主生产巨核细胞分化因子的过程。 本发明还提供了针对本发明的巨核细胞分化因子的特异性抗体。

公开(公告)号：US4948875A

公开(公告)日：1990-08-14

申请号：US123316

申请日：1987-11-20

申请人： Shoji Tanaka ,  Masafumi Tsujimoto ,  Yayoi Wada ,  Nobuo Tsuruoka ,  Hiroshi Nakazato

发明人： Shoji Tanaka ,  Masafumi Tsujimoto ,  Yayoi Wada ,  Nobuo Tsuruoka ,  Hiroshi Nakazato

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/20 ,  C07K14/00 ,  C07K14/52 ,  C07K14/525 ,  C12N15/00 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/525 ,  C12N15/70

摘要： Disclosed is a human TNF derivative with a reduced tendency to cause adverse side effects that is obtained by replacing the 31st and 32nd arginine residues from the N-terminal of the amino acid sequence of human TNF, which has a potential for causing serious adverse effects, with asparagine residue and threoine residue, respectively, as well as a method of preparing the same utilizing recombinant DNA technology.

摘要翻译： 公开了通过用可能引起严重不良反应的人TNF的氨基酸序列的N-末端替代第31位和32位精氨酸残基而导致副作用降低的趋势的人TNF衍生物， 分别使用天冬酰胺残基和苏氨酸残基，以及利用重组DNA技术制备其的方法。

公开(公告)号：US07790447B2

公开(公告)日：2010-09-07

申请号：US11658762

申请日：2005-07-27

申请人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

发明人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

IPC分类号： C12N15/36 ,  C12N9/00

CPC分类号： C12N9/13 ,  C07K2319/02 ,  C07K2319/21 ,  C12N9/18 ,  C12N2799/026 ,  C12N2840/105 ,  C12N2840/75

摘要： An object of the present invention is to provide an expression vector that allows for stable production of N-deacetylase/N-sulfotransferase 2 in large amounts and a process for production of N-deacetylase/N-sulfotransferase 2 using the same. The present invention provides a recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having lobster L21 DNA, DNA encoding gp67 signal peptide and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.

摘要翻译： 本发明的目的在于提供能够大量稳定生产N-脱乙酰酶/ N-磺基转移酶2的表达载体和使用其的N-脱乙酰酶/ N-磺基转移酶2的制造方法。 本发明提供了通过将杆状病毒DNA，具有龙虾L21 DNA的DNA片段，编码gp67信号肽的DNA和编码人N-脱乙酰酶/ N-磺基转移酶2的第79〜883位氨基酸的DNA并入本发明而获得的重组杆状病毒表达载体 在5'到3'的方向。

公开(公告)号：US5849557A

公开(公告)日：1998-12-15

申请号：US283917

申请日：1994-08-03

申请人： Hideki Adachi ,  Masafumi Tsujimoto ,  Keizo Inoue ,  Hiroyuki Arai

发明人： Hideki Adachi ,  Masafumi Tsujimoto ,  Keizo Inoue ,  Hiroyuki Arai

IPC分类号： C12N9/16 ,  C12N9/18 ,  C12N15/09 ,  C12N15/53 ,  C12N9/20

CPC分类号： C12N9/18

摘要： An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.

公开(公告)号：US5151349A

公开(公告)日：1992-09-29

申请号：US473168

申请日：1990-01-31

申请人： Shoji Tanaka ,  Masafumi Tsujimoto ,  Yayoi Wada ,  Nobuo Tsuruoka ,  Hiroshi Nakazato

发明人： Shoji Tanaka ,  Masafumi Tsujimoto ,  Yayoi Wada ,  Nobuo Tsuruoka ,  Hiroshi Nakazato

IPC分类号： C07K14/525 ,  C12N15/70

CPC分类号： C07K14/525 ,  C12N15/70

摘要： Disclosed is a human TNF derivative with a reduced tendency to cause adverse side effects that is obtained by replacing the 31st and 32nd arginine residues from the N-terminal of the amino acid sequence of human TNF, which has a potential for causing serious adverse effects, with asparagine residue and threoine residue, respectively, as well as a method of preparing the same utilizing recombinant DNA technology.

摘要翻译： 公开了通过用可能引起严重不良反应的人TNF的氨基酸序列的N-末端替代第31位和32位精氨酸残基而导致副作用降低的趋势的人TNF衍生物， 分别使用天冬酰胺残基和苏氨酸残基，以及利用重组DNA技术制备其的方法。

公开(公告)号：US4828990A

公开(公告)日：1989-05-09

申请号：US908707

申请日：1986-10-10

申请人： Naoki Higashi ,  Shunjiro Sugimoto ,  Masafumi Tsujimoto ,  Hounai Shirasawa ,  Tsutomu Okada ,  Kazumori Yamamoto ,  Tattanahalli L. Nagabhushan ,  Paul P. Trotta

发明人： Naoki Higashi ,  Shunjiro Sugimoto ,  Masafumi Tsujimoto ,  Hounai Shirasawa ,  Tsutomu Okada ,  Kazumori Yamamoto ,  Tattanahalli L. Nagabhushan ,  Paul P. Trotta

IPC分类号： C12N15/09 ,  A61K38/21 ,  C07K1/14 ,  C07K14/52 ,  C07K14/555 ,  C07K14/57 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/57 ,  Y10S435/811 ,  Y10S930/142

摘要： A method of purifying a polypeptide having a physiological activity such as one having interferon activities from a culture mixture of a microorganism obtained by a recombinant DNA technique and capable of producing the polypeptide is disclosed. The method comprises subjecting the cultured cells to extraction and purification in the presence of a salt of zinc or copper and polyethyleneimine thereby inhibiting decomposition and denaturation of the polypeptide. The extracted polypeptide can be further purified by column chromatographies using a column containing an anion exchange resin, column containing a cation exchange resin and column containing a gel filtration resin.

摘要翻译： PCT No.PCT / JP85 / 00715 Sec。 371日期1986年10月10日第 102（e）日期1986年10月10日PCT 1985年12月26日PCT PCT。 第WO86 / 04067号公报 日本1986年7月17日。一种纯化具有生理活性的多肽的方法，例如具有干扰素活性的多肽，其由通过重组DNA技术获得的能够产生该多肽的微生物的培养物混合物中进行。 该方法包括在锌或铜盐和聚乙烯亚胺的存在下使培养的细胞进行提取和纯化，从而抑制多肽的分解和变性。 提取的多肽可以使用含有阴离子交换树脂的柱，含有阳离子交换树脂的柱和含有凝胶过滤树脂的柱，通过柱色谱进一步纯化。