公开(公告)号：US20240016132A1

公开(公告)日：2024-01-18

申请号：US18353012

申请日：2023-07-14

申请人： Oxitec Limited

发明人： Martha Koukidou ,  Luke Alphey ,  Simon Warner

IPC分类号： A01K67/033 ,  C12N15/85 ,  C12Q1/6888

CPC分类号： A01K67/0339 ,  C12N15/8509 ,  C12Q1/6888 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/706 ,  A01K2267/02 ,  C12N2800/90 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2840/007 ,  C12N2840/44 ,  C12N2840/75 ,  C12N2999/007 ,  C12Q2600/158

摘要： Two or more conditional, dominant, lethal gene expression systems provide high levels of penetrance in insects. Lethality is induced at an earlier stage of development and the risk of biochemical resistance is reduced, as compared to a single insect conditional, dominant, lethal gene expression system. The invention is useful for the control of insect populations.

公开(公告)号：US20090053735A1

公开(公告)日：2009-02-26

申请号：US11658762

申请日：2005-07-27

申请人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

发明人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

IPC分类号： G01N33/573 ,  C12N9/10 ,  C12N15/00 ,  C12N5/00 ,  C12P21/04

CPC分类号： C12N9/13 ,  C07K2319/02 ,  C07K2319/21 ,  C12N9/18 ,  C12N2799/026 ,  C12N2840/105 ,  C12N2840/75

摘要： An object of the present invention is to provide an expression vector that allows for stable production of N-deacetylase/N-sulfotransferase 2 in large amounts and a process for production of N-deacetylase/N-sulfotransferase 2 using the same. The present invention provides a recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having lobster L21 DNA, DNA encoding gp67 signal peptide and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.

摘要翻译： 本发明的目的在于提供能够大量稳定生产N-脱乙酰酶/ N-磺基转移酶2的表达载体和使用其的N-脱乙酰酶/ N-磺基转移酶2的制造方法。 本发明提供了通过将杆状病毒DNA，具有龙虾L21 DNA的DNA片段，编码gp67信号肽的DNA和编码人N-脱乙酰酶/ N-磺基转移酶2的第79〜883位氨基酸的DNA并入本发明而获得的重组杆状病毒表达载体 在5'到3'的方向。

公开(公告)号：US11737436B2

公开(公告)日：2023-08-29

申请号：US16997416

申请日：2020-08-19

申请人： Oxitec Limited

发明人： Martha Koukidou ,  Luke Alphey ,  Simon Warner

IPC分类号： C12N15/85 ,  A01K67/033 ,  C12Q1/6888

CPC分类号： A01K67/0339 ,  C12N15/8509 ,  C12Q1/6888 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/706 ,  A01K2267/02 ,  C12N2800/90 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2840/007 ,  C12N2840/44 ,  C12N2840/75 ,  C12N2999/007 ,  C12Q2600/158

摘要： Two or more conditional, dominant, lethal gene expression systems provide high levels of penetrance in insects. Lethality is induced at an earlier stage of development and the risk of biochemical resistance is reduced, as compared to a single insect conditional, dominant, lethal gene expression system. The invention is useful for the control of insect populations.

公开(公告)号：US07790447B2

公开(公告)日：2010-09-07

申请号：US11658762

申请日：2005-07-27

申请人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

发明人： Jun-ichi Aikawa ,  Masafumi Tsujimoto ,  Takuji Kaneko

IPC分类号： C12N15/36 ,  C12N9/00

CPC分类号： C12N9/13 ,  C07K2319/02 ,  C07K2319/21 ,  C12N9/18 ,  C12N2799/026 ,  C12N2840/105 ,  C12N2840/75

摘要： An object of the present invention is to provide an expression vector that allows for stable production of N-deacetylase/N-sulfotransferase 2 in large amounts and a process for production of N-deacetylase/N-sulfotransferase 2 using the same. The present invention provides a recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having lobster L21 DNA, DNA encoding gp67 signal peptide and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.

摘要翻译： 本发明的目的在于提供能够大量稳定生产N-脱乙酰酶/ N-磺基转移酶2的表达载体和使用其的N-脱乙酰酶/ N-磺基转移酶2的制造方法。 本发明提供了通过将杆状病毒DNA，具有龙虾L21 DNA的DNA片段，编码gp67信号肽的DNA和编码人N-脱乙酰酶/ N-磺基转移酶2的第79〜883位氨基酸的DNA并入本发明而获得的重组杆状病毒表达载体 在5'到3'的方向。

公开(公告)号：US20170188559A1

公开(公告)日：2017-07-06

申请号：US15313922

申请日：2015-06-04

申请人： Oxitec Limited

发明人： Martha KOUKIDOU ,  Luke ALPHEY ,  Simon WARNER

IPC分类号： A01K67/033 ,  C12Q1/68 ,  C12N15/85

CPC分类号： A01K67/0339 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/706 ,  A01K2267/02 ,  C12N15/8509 ,  C12N2800/90 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2840/007 ,  C12N2840/44 ,  C12N2840/75 ,  C12N2999/007 ,  C12Q1/6888 ,  C12Q2600/158

摘要： Two or more conditional, dominant, lethal gene expression systems provide high levels of penetrance in insects. Lethality is induced at an earlier stage of development and the risk of biochemical resistance is reduced, as compared to a single insect conditional, dominant, lethal gene expression system. The invention is useful for the control of insect populations.

公开(公告)号：US20110207210A1

公开(公告)日：2011-08-25

申请号：US12322181

申请日：2009-01-31

申请人： Tzong-Yuan Wu ,  Ying-Ju Chen ,  Chao-Yi Teng ,  Yi-Ting Lin ,  Yu-Jie Chen ,  Yi-Jane Wu ,  Seng-Chi Chen

发明人： Tzong-Yuan Wu ,  Ying-Ju Chen ,  Chao-Yi Teng ,  Yi-Ting Lin ,  Yu-Jie Chen ,  Yi-Jane Wu ,  Seng-Chi Chen

IPC分类号： C12N15/866

CPC分类号： C07K14/005 ,  C12N2770/22022 ,  C12N2830/75 ,  C12N2840/203 ,  C12N2840/75

摘要： Provided herein are isolated or synthesized Rhopalosiphum padi virus (RhPV) nucleic acids having promotor activities, and vectors containing the isolated or synthesized RhPV nucleic acids for gene delivery in insect expression systems.

摘要翻译： 本文提供了具有启动子活性的分离或合成的罗非鱼属病毒（RhPV）核酸，以及含有用于昆虫表达系统中的基因递送的分离或合成的RhPV核酸的载体。