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    Anti-microbial proteins
    2.
    发明授权
    Anti-microbial proteins 失效
    抗微生物蛋白质

    公开(公告)号：US5607919A

    公开(公告)日：1997-03-04

    申请号：US543238

    申请日：1995-10-13

    申请人： Kirsten Bojsen ,  Karsten M. Kragh ,  J.o slashed.rn D. Mikkelsen ,  Klaus K. Nielsen ,  John E. Nielsen

    发明人： Kirsten Bojsen ,  Karsten M. Kragh ,  J.o slashed.rn D. Mikkelsen ,  Klaus K. Nielsen ,  John E. Nielsen

    IPC分类号： A01H5/00 ,  A01N65/00 ,  A01N65/08 ,  C07K1/20 ,  C07K14/00 ,  C07K14/37 ,  C07K14/41 ,  C07K14/415 ,  C07K19/00 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/29 ,  C12N15/56 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91

    CPC分类号： C12Y302/01014 ,  A01N65/08 ,  C07K14/415 ,  C12N15/8282 ,  C12N9/2402

    摘要： Anti-microbial proteins isolated from sugar beet, wherein the anti-microbial proteins exclude chitinases and glucanases. Said proteins include a pure protein selected from those depicted in SEQ ID Nos 2, 5 and 8, or a functionally equivalent analogue thereof in which one or more amino acids have been added, substituted or removed without substantially reducing the protein's anti-microbial activity, or mixtures of such proteins or analogues. A synergistic anti-fungal effect is observed if at least one of the AX proteins is combined with a WIN protein. The invention also provides recombinant DNA comprising a sequence encoding a protein according to the invention, a vector comprising said DNA and transformed plants comprising said DNA. The invention further provides an anti-microbial composition containing one or more of the said proteins, and a process for combatting fungi or bacteria which comprises exposing them to said proteins or compositions.

    摘要翻译： 从甜菜中分离的抗微生物蛋白质，其中抗微生物蛋白质排除几丁质酶和葡聚糖酶。 所述蛋白质包括选自SEQ ID No.2,5和8中描述的那些的纯蛋白质或其功能上等同的类似物，其中一个或多个氨基酸已被添加，取代或除去而不显着降低蛋白质的抗微生物活性， 或这些蛋白质或类似物的混合物。 如果将至少一种AX蛋白与WIN蛋白结合，则观察到协同的抗真菌作用。 本发明还提供了包含编码本发明蛋白质的序列的重组DNA，包含所述DNA的载体和包含所述DNA的转化植物。 本发明还提供了含有一种或多种所述蛋白质的抗微生物组合物，以及用于对抗真菌或细菌的方法，其包括将其暴露于所述蛋白质或组合物。

    Process of obtaining ethanol without glucoamylase using Pseudomonas saccharophila G4-amylase variants thereof
    10.
    发明授权
    Process of obtaining ethanol without glucoamylase using Pseudomonas saccharophila G4-amylase variants thereof 失效
    使用酿酒酵母G4-淀粉酶变体获得没有葡糖淀粉酶的乙醇的方法

    公开(公告)号：US08318451B2

    公开(公告)日：2012-11-27

    申请号：US12318513

    申请日：2008-12-30

    申请人： Andrew Shaw ,  Regina Chin ,  Karsten M. Kragh

    发明人： Andrew Shaw ,  Regina Chin ,  Karsten M. Kragh

    IPC分类号： C12Q1/40 ,  C12N9/26 ,  C12N9/28 ,  C07K14/00 ,  C12P21/00

    CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23L29/35 ,  Y02E50/16 ,  Y02E50/17

    摘要： Pseudomonas saccharophila G4-forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.

    摘要翻译： 糖酵母（Saccharophila）G4形成淀粉酶（PS4）及其变体有利地可用于酶催化的高温液化步骤以从淀粉例如玉米淀粉生产乙醇。 PS4产生显着量的麦芽三糖，其可以在随后的发酵步骤中被酿酒酵母用于产生乙醇。 PS4的这种性质有利地允许在没有糖化步骤的情况下从液化淀粉生产乙醇。 提供了PS4变体，其具有改进的性质，例如热稳定性和/或改变的外切特异性和内切特异性淀粉酶活性。