公开(公告)号：US20070148651A1

公开(公告)日：2007-06-28

申请号：US10582637

申请日：2004-11-12

Applicant: Uwe Michelsen ,  Karl Holschuh ,  Achim Schwammle

Inventor： Uwe Michelsen ,  Karl Holschuh ,  Achim Schwammle

IPC: C12Q1/68 ,  C12N1/08

CPC classification number: C12N15/1013

Abstract: The present invention relates to a method and a kit for the isolation of RNA in the presence of DNA. The isolation is carried out by binding to a magnetite solid phase, where the binding buffer produces a concentration of guanidinium thiocyanate of>2.5 mol/l, and a certain concentration of phosphate is present during the binding.

Abstract translation: 本发明涉及在DNA存在下分离RNA的方法和试剂盒。 通过结合磁铁矿固相进行分离，其中结合缓冲液产生> 2.5mol / l的硫氰酸胍浓度，并且在结合期间存在一定浓度的磷酸盐。

公开(公告)号：US20110008797A1

公开(公告)日：2011-01-13

申请号：US12922470

申请日：2009-03-19

Applicant: Christian Zilch ,  Wilhelm Gerdes ,  Johann Bauer ,  Karl Holschuh

Inventor： Christian Zilch ,  Wilhelm Gerdes ,  Johann Bauer ,  Karl Holschuh

IPC: C12Q1/68 ,  C12P19/34 ,  C12M1/00 ,  C12M1/34

CPC classification number: H01F1/0063 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/14 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2300/1894 ,  B82Y5/00 ,  B82Y25/00 ,  C12N15/1096 ,  H01F1/0054 ,  H01F1/37 ,  H01F1/407

Abstract: The invention relates to a method for the thermal control of at least one temperature-dependent enzymatic reaction in the presence of magnetic particles, particularly nanoparticles, or magnetic beads in vitro by means of the heating of the magnetic beads or magnetic particles to at least one certain target temperature by means of alternating magnetic fields. The enzymatic reaction which can be controlled with the method according to the invention is preferably a PCR reaction or another reaction for the elongation or amplification of nucleic acids, including DNA, RNA or hybrids or derivatives thereof, which takes place directly on the functionalized magnetic beads. Further aspects of the invention relate to a reactor for carrying out the method and the use of the method or the reactor in analytics and diagnostics.

Abstract translation: 本发明涉及一种用于在磁性颗粒，特别是纳米颗粒或磁珠的存在下，通过将磁珠或磁性颗粒加热至少至少一种在体外热控制至少一种温度依赖性酶反应的方法 某些目标温度通过交变磁场。 可用本发明方法控制的酶反应优选是直接在功能化磁珠上进行的PCR反应或用于延伸或扩增核酸（包括DNA，RNA或其杂合物或衍生物）的另一反应 。 本发明的其它方面涉及用于在分析和诊断中执行该方法和使用该方法或反应器的反应器。

公开(公告)号：US06548253B1

公开(公告)日：2003-04-15

申请号：US09830956

申请日：2001-06-08

Applicant: Karl Holschuh ,  Uwe Michelsen

Inventor： Karl Holschuh ,  Uwe Michelsen

IPC: C12Q168

CPC classification number: C12N15/1006 ,  C12N15/1013

Abstract: The invention relates to a method of isolating plasmid DNA from microorganism cultures with the aid of solid-phase bodies. The solid-phase bodies can be silica gels, silicates, or glass-like materials and the solid-phase bodies can have magnetic properties. The solid-phase bodies are used for isolating the microorganisms and for isolating the plasmid DNA.

Abstract translation: 本发明涉及借助于固相体从微生物培养物中分离质粒DNA的方法。 固相体可以是硅胶，硅酸盐或玻璃状材料，固相体可以具有磁性。 固相体用于分离微生物和分离质粒DNA。

公开(公告)号：US20130122517A1

公开(公告)日：2013-05-16

申请号：US13811743

申请日：2011-06-30

Applicant: Tanja Henzler ,  Karl Holschuh ,  Johann Bauer ,  Thomas Herget ,  Michael Hartmann ,  Oliver Poetz ,  Thomas Joos

Inventor： Tanja Henzler ,  Karl Holschuh ,  Johann Bauer ,  Thomas Herget ,  Michael Hartmann ,  Oliver Poetz ,  Thomas Joos

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  B82Y15/00 ,  G01N33/54346 ,  G01N33/588

Abstract: The present invention relates to a method and kit for performing assays like immunoassays. The assays are performed by using two different types of magnetic beads.

Abstract translation: 本发明涉及用于进行如免疫测定法的测定的方法和试剂盒。 通过使用两种不同类型的磁珠来进行测定。

公开(公告)号：US06355792B1

公开(公告)日：2002-03-12

申请号：US09601397

申请日：2000-08-02

Applicant: Uwe Michelsen ,  Karl Holschuh ,  Robertus Hendriks

Inventor： Uwe Michelsen ,  Karl Holschuh ,  Robertus Hendriks

IPC: C07H2100

CPC classification number: C12N15/1006

Abstract: The invention provides a method for isolating and purifying nucleic acids from liquid samples. The method comprises using a solid carrier which is essentially made of oxide inorganic materials containing hydroxyl groups (e.g., silica gel or hydroxylapatite). The nucleic acids are bound to the carrier material in the acidic pH range while they are eluted in the alkaline pH range.

Abstract translation: 本发明提供了从液体样品中分离和纯化核酸的方法。 该方法包括使用基本上由含有羟基的氧化物无机材料（例如硅胶或羟基磷灰石）制成的固体载体。 核酸在酸性pH范围内与载体材料结合，同时它们在碱性pH范围内洗脱。