公开(公告)号：US5696314A

公开(公告)日：1997-12-09

申请号：US679089

申请日：1996-07-12

申请人： Robert McCaffrey ,  Bonnie Dalzell ,  Katarina Tkacik ,  Susan Holbert ,  Mark Boden ,  James Flaherty ,  Michael Flanagan ,  Josef Brown ,  Kimiya Takeshita ,  Peter Edelman

发明人： Robert McCaffrey ,  Bonnie Dalzell ,  Katarina Tkacik ,  Susan Holbert ,  Mark Boden ,  James Flaherty ,  Michael Flanagan ,  Josef Brown ,  Kimiya Takeshita ,  Peter Edelman

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26 ,  G01N33/535 ,  G01N33/66 ,  H01L29/78

CPC分类号： C12Q1/002 ,  Y10S435/817

摘要： An electrochemical sensor is designed for use in detecting a concentration of an analyte in a sample solution. The sensor to have a longer useful lifetime, improved stability and improved response time for single or multiple use applications. In one aspect, some of these goals are achieved by providing the multilayer enzyme electrode membrane having both nonporous and microporous layers. The microporous layer is formed from a polymer binder, a polymer powder, a surfactant and a mineral powder. In another aspect, certain of these goals are achieved by the use of a layer comprised of an enzyme disposed within a polymer matrix. In certain embodiments, the multilayer enzyme electrode membrane includes an outermost layer which is capable of dissolving in the sample solution such that the interface between the multilayer enzyme electrode membrane and the sample solution is continually renewed. The sensor provides an electrical current output proportional to the reagent chemical interaction of the analyte with an immobilized enzyme layer during exposure of the sensor in such solution.

摘要翻译： 电化学传感器设计用于检测样品溶液中分析物的浓度。 该传感器具有更长的使用寿命，提高了单个或多个使用应用的稳定性和改进的响应时间。 在一个方面，通过提供具有无孔和多孔层的多层酶电极膜来实现这些目标中的一些。 微孔层由聚合物粘合剂，聚合物粉末，表面活性剂和矿物粉末形成。 在另一方面，这些目标中的某些通过使用由置于聚合物基质内的酶构成的层来实现。 在某些实施方案中，多层酶电极膜包括能够溶解在样品溶液中的最外层，使得多层酶电极膜和样品溶液之间的界面不断更新。 传感器在这种溶液中曝光期间，传感器提供与分析物与固定化酶层的试剂化学相互作用成比例的电流输出。

公开(公告)号：US5707502A

公开(公告)日：1998-01-13

申请号：US680050

申请日：1996-07-12

申请人： Robert McCaffrey ,  Katarina Tkacik ,  Brian Holman ,  James Flaherty ,  Josef Brown ,  Peter Edelman

发明人： Robert McCaffrey ,  Katarina Tkacik ,  Brian Holman ,  James Flaherty ,  Josef Brown ,  Peter Edelman

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  C12Q1/002 ,  Y10S435/817

摘要： A sensor for measuring a concentration of an analyte in a solution. The sensor comprises a substrate layer, an electrode layer and an immobilized enzyme layer. The sensor further includes an enzyme/polymer layer and/or a hydrophobic layer. The enzyme/polymer layer includes an enzyme disposed within a polymer matrix. The hydrophobic layer is formed from a material that is more hydrophobic than the immobilized enzyme layer material. In certain embodiments, the sensor includes the enzyme/polymer layer and the hydrophobic layer disposed between the enzyme/polymer layer and the immobilized enzyme layer such that the enzyme/polymer layer is disposed along the hydrophobic layer within an area above the immobilized enzyme layer.

摘要翻译： 用于测量溶液中分析物浓度的传感器。 传感器包括基底层，电极层和固定化酶层。 传感器还包括酶/聚合物层和/或疏水层。 酶/聚合物层包括设置在聚合物基质内的酶。 疏水层由比固定化酶层材料更疏水的材料形成。 在某些实施方案中，传感器包括酶/聚合物层和设置在酶/聚合物层和固定化酶层之间的疏水层，使得酶/聚合物层沿着疏水层设置在固定化酶层上方的区域内。