-
公开(公告)号：US08158349B2
公开(公告)日：2012-04-17
申请号：US13006928
申请日：2011-01-14
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
公开(公告)号：US20120152818A1
公开(公告)日：2012-06-21
申请号：US13410161
申请日：2012-03-01
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
IPC: B01D29/50
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
公开(公告)号：US20050208548A1
公开(公告)日：2005-09-22
申请号：US11053065
申请日：2005-02-07
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
Patent Agency Ranking
公开(公告)号：US08927261B2
公开(公告)日：2015-01-06
申请号：US13410161
申请日：2012-03-01
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
IPC: C12M1/34 , C12M3/00 , C12Q1/68 , C12P19/34 , C12M1/00 , G01N15/06 , G01N33/00 , G01N33/48 , C07H21/02 , B01L3/00 , C12N15/10
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
公开(公告)号：US20110137020A1
公开(公告)日：2011-06-09
申请号：US13006928
申请日：2011-01-14
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
IPC: C07H1/06
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
公开(公告)号：US07897378B2
公开(公告)日：2011-03-01
申请号：US11053065
申请日：2005-02-07
Applicant: Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
Inventor： Dirk Block , Ruediger Haenel , Joerg Kleiber , Manuela Poignee-Heger , Thomas Walter , Ralf Zielenski
CPC classification number: B01L3/5021 , B01L2300/0609 , B01L2300/0681 , C12N15/1017
Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.
Abstract translation: 本发明涉及一种从样品中分离和纯化核酸的方法，因此适用于此。
-
公开(公告)号：US20060058518A1
公开(公告)日：2006-03-16
申请号：US10540406
申请日：2004-01-28
Applicant: Frank Bergmann , Christine Market-Hahn , Joerg Kleiber , Dirk Block
Inventor： Frank Bergmann , Christine Market-Hahn , Joerg Kleiber , Dirk Block
CPC classification number: C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.
Abstract translation: 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。
-
公开(公告)号：US5474916A
公开(公告)日：1995-12-12
申请号：US950849
申请日：1992-09-28
Applicant: Udo Reischl , Ruediger Rueger , Cortina Kaletta , Christoph Kessler , Joerg Kleiber
Inventor： Udo Reischl , Ruediger Rueger , Cortina Kaletta , Christoph Kessler , Joerg Kleiber
CPC classification number: C12Q1/6865
Abstract: Process for the specific production of nucleic acids based on the principle of transcription in which a promoter oligonucleotide and a template-specific oligonucleotide which can hybridize with it are used as a promoter reagent and a process for nucleic acid detection which is based on this process.
Abstract translation: 基于转录原理进行特异性生产核酸的方法，其中使用可与其杂交的启动子寡核苷酸和模板特异性寡核苷酸作为启动子试剂和基于该方法的核酸检测方法。
-
-
-
-
-
-
-
Search Results
8
records
(took 0.015 seconds)
