公开(公告)号：US20120010275A1

公开(公告)日：2012-01-12

申请号：US13235404

申请日：2011-09-18

Applicant: James W. Ryan

Inventor： James W. Ryan

IPC: A61K48/00 ,  C12N15/63 ,  C12N1/21 ,  C12Q1/68 ,  C12N5/04 ,  C12N1/15 ,  C12P21/00 ,  C12N15/12 ,  C12N5/10

CPC classification number: C12Q1/6883 ,  A61K2039/53 ,  C07K14/47 ,  C12Q1/6876 ,  C12Q2600/156 ,  Y10T436/143333

Abstract: Provided herein are isolated genomic polynucleotide fragments from the p15 arm of chromosome 11 and methods of use.

Abstract translation: 本文提供了来自染色体11的p15臂的分离的基因组多核苷酸片段和使用方法。

公开(公告)号：US08057998B1

公开(公告)日：2011-11-15

申请号：US12276550

申请日：2008-11-24

Applicant: James W Ryan

Inventor： James W Ryan

IPC: C12Q1/68 ,  C12N15/00 ,  C12P21/06 ,  C07H21/02

CPC classification number: C07K14/47 ,  C07K14/4716

Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human resistin and human syntaxin binding protein 2, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human resistin and human syntaxin binding protein 2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

Abstract translation: 本发明涉及编码人抵抗素和人类胃蛋白酶结合蛋白2的分离的基因组多核苷酸片段，含有这些片段的载体和宿主以及与非编码区杂交的片段以及与这些片段的反义寡核苷酸。 本发明进一步涉及使用这些片段获得人抵抗素和人类突触蛋白结合蛋白2并诊断，治疗，预防和/或改善病理性病症的方法。

公开(公告)号：US07964357B1

公开(公告)日：2011-06-21

申请号：US12795864

申请日：2010-06-08

Applicant: James W Ryan

Inventor： James W Ryan

IPC: C12Q1/68 ,  C07H21/02

CPC classification number: A61K31/7088 ,  C12N9/48 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Y304/16005

Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

Abstract translation: 本发明涉及编码人类羧肽酶M的分离的基因组多核苷酸片段和含有这些片段的人类小鼠双分子2同系物，载体和宿主，并且与非编码区杂交的片段以及与这些片段的反义寡核苷酸杂交。 本发明进一步涉及使用这些片段获得人类羧肽酶M和人类小鼠双分钟2同系物并诊断，治疗，预防和/或改善病理性疾病的方法。

公开(公告)号：US20090324627A1

公开(公告)日：2009-12-31

申请号：US12533164

申请日：2009-07-31

Applicant: James W. Ryan

Inventor： James W. Ryan

IPC: A61K39/395 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12P21/02 ,  A61K31/7088 ,  C40B40/08 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  A61K38/00 ,  C07K14/705 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/48 ,  Y10T436/143333

Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human SNARE YKT6, human glucokinase, human adipocyte enhancer binding protein (AEBP1) and DNA directed 50 kD regulatory subunit (POLD2), vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain SNARE YKT6, human glucokinase, AEBP1 protein and POLD2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

Abstract translation: 本发明涉及编码人类SNARE YKT6，人类葡萄糖激酶，人类脂肪细胞增强子结合蛋白（AEBP1）和定向的50kD调节亚基（POLD2）的DNA的分离的基因组多核苷酸片段，含有这些片段的载体和宿主以及与非编码区杂交的片段 以及这些片段的反义寡核苷酸。 本发明进一步涉及使用这些片段获得SNARE YKT6，人类葡萄糖激酶，AEBP1蛋白和POLD2并诊断，治疗，预防和/或改善病理性疾病的方法。

公开(公告)号：US07588915B2

公开(公告)日：2009-09-15

申请号：US10642946

申请日：2003-08-18

Applicant: James W. Ryan

Inventor： James W. Ryan

IPC: C12P21/06 ,  C12Q1/68 ,  C12N15/63 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6876 ,  A61K38/00 ,  C07K14/705 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/48 ,  Y10T436/143333

Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human SNARE YKT6, human glucokinase, human adipocyte enhancer binding protein (AEBP1) and DNA directed 50 kD regulatory subunit (POLD2), vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain SNARE YKT6, human glucokinase, AEBP1 protein and POLD2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

Abstract translation: 本发明涉及编码人类SNARE YKT6，人类葡萄糖激酶，人类脂肪细胞增强子结合蛋白（AEBP1）和定向的50kD调节亚基（POLD2）的DNA的分离的基因组多核苷酸片段，含有这些片段的载体和宿主以及与非编码区杂交的片段 以及这些片段的反义寡核苷酸。 本发明进一步涉及使用这些片段获得SNARE YKT6，人类葡萄糖激酶，AEBP1蛋白和POLD2并诊断，治疗，预防和/或改善病理性疾病的方法。

公开(公告)号：US4745124A

公开(公告)日：1988-05-17

申请号：US515474

申请日：1983-07-20

Applicant: James W. Ryan ,  Alfred Chung

Inventor： James W. Ryan ,  Alfred Chung

IPC: A61K38/00 ,  C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07K5/068 ,  C07K5/078 ,  A61K31/40 ,  A61K31/41 ,  C07D207/00 ,  C07D211/72

CPC classification number: C07K5/06173 ,  C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07K5/06086 ,  A61K38/00

Abstract: Novel thiolester compounds are disclosed which are orally effective angiotensin converting enzyme inhibitors useful in the treatment of mammalian hypertension. They have the formula,R.sub.1 -A.sub.1 -S-Z,wherein Z denotes -B-A.sub.2, R.sub.1 is H or an acyl group, A.sub.1 is a carboxylic acid containing at least one amino or imino -N-, A.sub.2 is a carboxylic acid containing at least one amino or imino -N- or a lower alkyl ester or amide thereof, B is a 2-4 carbon backbone chain in mercapto linkage to S which includes a carbonyl or sulfonyl group joined in carboxamido or sulfonamido linkage, respectively, to A.sub.2.Preferably A.sub.2 includes a 4-6 membered C-N ring or a 5 membered ring of one N, one S and 3 C atoms.

Abstract translation: 公开了新颖的硫醇酯化合物，其是可用于治疗哺乳动物高血压的口服有效的血管紧张素转换酶抑制剂。 它们具有式R1-A1-SZ，其中Z表示-B-A2，R1是H或酰基，A1是含有至少一个氨基或亚氨基-N-的羧酸，A2是含有 至少一个氨基或亚氨基-N-或其低级烷基酯或酰胺，B是与S巯基连接的2-4碳主链，其包括分别连接到甲酰氨基或亚磺酰氨基键的羰基或磺酰基。 优选地，A2包括一个N，一个S和3个C原子的4-6元C-N环或5元环。

公开(公告)号：US4695577A

公开(公告)日：1987-09-22

申请号：US64901

申请日：1979-08-14

Applicant: James W. Ryan ,  Alfred Chung

Inventor： James W. Ryan ,  Alfred Chung

IPC: A61K38/00 ,  C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07K5/068 ,  C07K5/078 ,  A61K31/40 ,  A61K31/41 ,  C07D207/00 ,  C07D211/72

CPC classification number: C07K5/06173 ,  C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07K5/06086 ,  A61K38/00

Abstract: Novel compounds are disclosed as potent inhibitors of angiotensin converting enzyme and as orally effective antihypertensive agents. The compounds have the general formula: ##STR1##

公开(公告)号：US4692437A

公开(公告)日：1987-09-08

申请号：US156749

申请日：1980-06-05

Applicant: James W. Ryan ,  Alfred Chung

Inventor： James W. Ryan ,  Alfred Chung

IPC: A61K38/55 ,  A61K20060101 ,  A61K31/40 ,  A61K31/401 ,  A61K31/405 ,  A61K38/00 ,  A61P9/12 ,  A61P43/00 ,  C07C20060101 ,  C07C67/00 ,  C07C325/00 ,  C07C327/20 ,  C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07D403/12 ,  C07K1/113 ,  C07K5/06 ,  C07K5/068 ,  C07K5/078 ,  C07K5/08 ,  C07K14/81 ,  A61K31/425 ,  C07D207/00 ,  C07D277/04

CPC classification number: C07D207/16 ,  C07D207/22 ,  C07D207/50 ,  C07K5/06086 ,  C07K5/06173 ,  A61K38/00 ,  Y10S930/12

Abstract: Inhibitors of angiotensin converting enzyme which are physiologically acceptable salts of compounds which have the formula: ##STR1## wherein R is hydrogen, formyl, acetyl, propanoyl, butanoyl, phenylacetyl, phenylpropanoyl, benzoyl, cyclopentanecarbonyl, tert-butyloxycarbonyl, cyclopentanecarbonyl-L-lysyl, pyro-L-glutamyl-L-lysyl, L-lysyl, L-arginyl or pyro-L-glutamyl;A is phenylalanyl, glycyl, alanyl, tryptophyl, tyrosyl, isoleucyl, leucyl, histidyl, or valyl, the .alpha.-amino group thereof being in amide linkage with R;R.sub.1 is hydrogen or methyl;R.sub.2 is L-proline, L-3,4-dehydroproline, D,L-3,4-dehydroproline, L-3-hydroxyproline, L-4-hydroxyproline, L-thiazolidine-4-carboxylic acid, or L-5-oxo-proline, the imino group thereof being in imide linkage with the adjacent ##STR2## and, n is 0 or 1, such that when n is O, R.sub.1 is methyl are disclosed as useful anti-hypertensive agents.

Abstract translation: 其中R为氢，甲酰基，乙酰基，丙酰基，丁酰基，苯乙酰基，苯基丙酰基，苯甲酰基，环戊烷羰基，叔丁氧基羰基，环戊烷羰基-L-赖氨酰基的化合物的生理上可接受的盐的血管紧张素转换酶抑制剂 ，pyro-L-谷氨酰-L-赖氨酰，L-赖氨酰，L-精氨酰或焦-L-谷氨酰; A是与R的酰胺键合的苯丙氨酰基，甘氨酰基，丙氨酰基，色氨酸，酪氨酰基，异亮氨酰基，亮氨酰基，组氨酰基或缬氨酰基; R1是氢或甲基; R2是L-脯氨酸，L-3,4-脱氢脯氨酸，D，L-3,4-脱氢脯氨酸，L-3-羟脯氨酸，L-4-羟基脯氨酸，L-噻唑烷-4-羧酸或L-5- 氧基 - 脯氨酸，其亚氨基与相邻的“IMAGE”具有酰亚胺键，并且n为0或1，使得当n为O时，R 1为甲基作为有用的抗高血压剂公开。

公开(公告)号：US4372941A

公开(公告)日：1983-02-08

申请号：US184653

申请日：1980-09-05

Applicant: James W. Ryan

Inventor： James W. Ryan

IPC: C07K5/087 ,  C12Q1/37 ,  A61K43/00

CPC classification number: C07K5/0812 ,  C12Q1/37

Abstract: A rapid, efficient and accurate method is provided for quantitatively assaying for an enzyme, such as angiotensin converting enzyme, an aminopeptidase, a carboxypeptidase, or trypsin, which catalyzes hydrolysis of a radiolabelled substrate to produce a radiolabelled remnant product preferentially soluble in a water-immiscible organic solvent, whereby the assay is conducted in a vial having a volume of not more than 20 milliliters, preferably 7 ml. or less, by incubating substrate in buffer and sample, stopping the reaction after a predetermined time with, e.g., acid or base, adding the water-immiscible organic solvent containing scintillant, capping the vial, mixing by inverting the vial whereupon a spontaneous phase separation occurs. The radioactivity of the organic phase is then counted in an appropriate scintillation counter and the enzymic activity is thereupon calculated.

Abstract translation: 提供了一种快速，有效和准确的方法来定量测定酶，例如血管紧张素转换酶，氨基肽酶，羧肽酶或胰蛋白酶，其催化放射性标记的底物水解产生优选溶于水溶性的放射性标记的残留产物， 不混溶的有机溶剂，其中测定在体积不大于20毫升，优选7毫升的小瓶中进行。 或以下，通过将缓冲液和样品中的底物孵育，在例如酸或碱等预定时间后停止反应，加入含有闪烁体的与水不混溶的有机溶剂，盖上小瓶，通过翻转小瓶进行混合，从而自发相分离 发生。 然后在合适的闪烁计数器中计数有机相的放射性，并计算酶活性。

公开(公告)号：US4335041A

公开(公告)日：1982-06-15

申请号：US191029

申请日：1980-09-26

Applicant: James W. Ryan ,  Alfred Chung

Inventor： James W. Ryan ,  Alfred Chung

IPC: C07K5/087 ,  C12Q1/37 ,  C07C103/52

CPC classification number: C12Q1/37 ,  C07K5/0812

Abstract: A method for measuring the activity of angiotensin converting enzyme is disclosed. In this assay, benzoylphenylalanylalanyproline or a radioisotope labelled form thereof is used as the substrate for the angiotensin converting enzyme.

Abstract translation: 公开了一种测定血管紧张素转换酶活性的方法。 在该测定中，使用苯甲酰苯丙氨酰脯氨酸或其放射性同位素标记形式作为血管紧张素转化酶的底物。