Abstract:
The preferred embodiments are directed to a method and apparatus of operating a scanning probe microscope (SPM) to perform sample measurements using a survey scan that is less than five lines, and more preferably two lines, to accurately locate a field of features of a sample. This is accomplished by selecting a step distance between adjacent lines of the survey scan that does not equal the pitch of the features in a direction orthogonal to the direction the survey scan traverses, i.e., does not equal the pitch of the features in the scan direction, XPO. The aspect ratio of the scans can also be modified to further improve sample throughput.
Abstract:
The identification of a novel gene, termed IκBNS, regulated during negative selection of immature CD4+CD8+double positive (DP) thymocytes in mice, and the human homolog of the gene, are described, along with the gene products produced by these genes. Methods of using the disclosed genes and gene products are also described.