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公开(公告)号：US09080139B2
公开(公告)日：2015-07-14
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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2.发明授权Buffer solution for electroporation and a method comprising the use of the same 有权用于电穿孔的缓冲溶液和包括其使用的方法公开(公告)号：US08192990B2
公开(公告)日：2012-06-05
申请号：US13009024
申请日：2011-01-19
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Mueller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Mueller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
摘要： A method for introducing biologically active molecules into animal or human cells using an electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution including HEPES and at least 10 mmol×1−1 magnesium ions (Mg2+), the buffer solution having a buffer capacity of at least 20 mmol×1−1 ×pH−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C., and an ionic strength of at least 200 mmol×1−1. An electric voltage is applied to the suspension.
摘要翻译： 使用电流将生物活性分子引入动物或人细胞的方法包括悬浮细胞并将生物活性分子溶解在包括HEPES和至少10mmol×1-1的镁离子（Mg 2+）的缓冲溶液中，缓冲溶液 在pH7至pH8和25℃的温度下，缓冲容量至少为20mmol×1-1×pH-1，离子强度为至少200mmol×1 -1。 向悬架施加电压。
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公开(公告)号：US20120087841A1
公开(公告)日：2012-04-12
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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公开(公告)号：US08058042B2
公开(公告)日：2011-11-15
申请号：US12766273
申请日：2010-04-23
IPC分类号： C12N13/00
CPC分类号： A61N1/0412 , A61N1/327 , C12N15/87 , H03K3/57
摘要： Disclosed is a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. A controller for monitoring the chronological progression of the voltage pulse is provided which controls at least one continuation of discharge after termination. Biomaterial is treated by using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse.
摘要翻译： 公开了一种电路装置，其包括用于电荷的至少一个存储装置，以通过选择性地放电存储装置产生至少一个电压脉冲，以及至少一个用于控制放电的控制单元。 提供用于监视电压脉冲的时间顺序的控制器，其控制终止后至少一次续航。 通过使用由第一电压脉冲产生的至少一个电场来处理生物材料，一旦电参数的值已经超过或低于预设极限，该第一电压脉冲终止。 在第一个电压脉冲终止后，通过附加的电压脉冲继续。
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公开(公告)号：US20100267106A1
公开(公告)日：2010-10-21
申请号：US12766273
申请日：2010-04-23
CPC分类号： A61N1/0412 , A61N1/327 , C12N15/87 , H03K3/57
摘要： The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse, said controller controlling at least one continuation of discharge after termination.
摘要翻译： 本发明涉及一种使用由第一电压脉冲产生的至少一个电场来处理生物材料的方法，一旦电参数的值已经超过或低于预设极限，该第一电压脉冲终止。 在第一个电压脉冲终止后，通过附加的电压脉冲继续。 本发明还涉及一种电路装置，其包括至少一个用于电荷的存储装置，以通过选择性地放电存储装置产生至少一个电压脉冲，以及至少一个用于控制放电的控制单元。 本发明提供了一种用于监视电压脉冲的时间顺序的控制器，所述控制器在终止后控制至少一次放电继续。
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公开(公告)号：US07732175B2
公开(公告)日：2010-06-08
申请号：US11151643
申请日：2005-06-14
IPC分类号： C12N13/00
CPC分类号： A61N1/0412 , A61N1/327 , C12N15/87 , H03K3/57
摘要： The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse. This controller controls at least one continuation of discharge after termination.
摘要翻译： 本发明涉及一种使用由第一电压脉冲产生的至少一个电场来处理生物材料的方法，一旦电参数的值已经超过或低于预设极限，该第一电压脉冲终止。 在第一个电压脉冲终止后，通过附加的电压脉冲继续。 本发明还涉及一种电路装置，其包括至少一个用于电荷的存储装置，以通过选择性地放电存储装置产生至少一个电压脉冲，以及至少一个用于控制放电的控制单元。 本发明提供一种用于监视电压脉冲的时间顺序的控制器。 该控制器在终止后至少控制一次放电继续。
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公开(公告)号：US07700357B2
公开(公告)日：2010-04-20
申请号：US11427474
申请日：2006-06-29
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61N1/0412 , A61N1/327
摘要： Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration.The biological material is added to at most 50 μl of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 μs, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 μs and is then again continued.
摘要翻译： 公开了一种通过电流处理生物材料的方法，其中将生物材料加入到具有相对高的离子强度的小体积的缓冲溶液中。 通过具有预设持续时间的高电压脉冲在缓冲溶液中产生强电场。 将生物材料加入至多50μl具有至少100mmol / l的离子强度的缓冲溶液。 通过具有至少10μs的预设持续时间的至少一个电压脉冲，在缓冲溶液中产生场强至少为1kV / cm的电场。 电压脉冲因此在至少100μs的持续时间中断至少一次，然后再次继续。
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公开(公告)号：US20060094095A1
公开(公告)日：2006-05-04
申请号：US11151643
申请日：2005-06-14
CPC分类号： A61N1/0412 , A61N1/327 , C12N15/87 , H03K3/57
摘要： The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse, said controller controlling at least one continuation of discharge after termination.
摘要翻译： 本发明涉及一种使用由第一电压脉冲产生的至少一个电场来处理生物材料的方法，一旦电参数的值已经超过或低于预设极限，该第一电压脉冲终止。 在第一个电压脉冲终止后，通过附加的电压脉冲继续。 本发明还涉及一种电路装置，其包括至少一个用于电荷的存储装置，以通过选择性地放电存储装置产生至少一个电压脉冲，以及至少一个用于控制放电的控制单元。 本发明提供了一种用于监视电压脉冲的时间顺序的控制器，所述控制器在终止后控制至少一次放电继续。
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公开(公告)号：US06390144B1
公开(公告)日：2002-05-21
申请号：US09855187
申请日：2001-05-14
申请人： Adnan Wahhoud , Peter Czura , Herbert Mueller , Fritz Rupflin , Thomas Laukamp
发明人： Adnan Wahhoud , Peter Czura , Herbert Mueller , Fritz Rupflin , Thomas Laukamp
IPC分类号： D03D3922
摘要： Ground warp and pile warp thread sheets are supplied to a shed forming device. The ground warp and the pile warp are separately deflected over a deflecting rod upstream from the shed forming device. The deflecting rod supports the ground warp and directs the pile warp to cross and interpenetrate through the ground warp before deflecting the pile warp into the back shed. The deflecting rod is positively driven and controlled to pivot about a horizontal axis to provide a positive controlled partial compensation of the pile warp thread length variations. The pile warp also runs over a pile warp thread reserve and compensating device upstream from the thread crossing location to provide an additional passive uncontrolled compensation of the pile warp thread length variations and to provide a pile warp thread reserve during the pile loop formation and/or the shed changes in the weaving process.
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10.发明授权Arrangement for controlling the movement of a rearward-side air guiding system on motor vehicles 失效用于控制机动车辆后方导风系统的运动的装置公开(公告)号：US06382708B1
公开(公告)日：2002-05-07
申请号：US09822844
申请日：2001-04-02
IPC分类号： B62D3702
CPC分类号： B62D35/007 , B62D37/02 , Y10S180/903
摘要： Arrangement for controlling the movement of a rearward-side air guiding system on motor vehicles, the rearward-side air guiding system having a rear spoiler provided on a rearward-side hood and a rear wing connected therewith, and the rear wing being movable by means of the arrangement from a retracted inoperative position into an extended operative position and vice versa. The arrangement for controlling the movement of the rear wing includes at least two telescopic tilt-out devices and one connection respectively between a driving device and the telescopic tilt-out device. The driving device contains a motor and at least one hydraulic cylinder, so that, when the motor (17) is triggered, a pressure is built up in the at least one hydraulic cylinder (18,18′) which can be transmitted to the telescopic tilt-out devices (16,16′) and has the effect that the rear wing (12) is moved against a spring force into its operative position.
摘要翻译： 用于控制机动车辆后方空气引导系统的运动的布置，后侧空气引导系统具有设置在后侧罩上的后扰流板和与其连接的后翼，后翼可通过装置移动 从缩回的非操作位置到延伸的操作位置，反之亦然。 用于控制后翼的运动的装置包括分别在驱动装置和伸缩倾斜装置之间的至少两个伸缩倾斜装置和一个连接。 所述驱动装置包括马达和至少一个液压缸，使得当所述马达（17）被触发时，在所述至少一个液压缸（18,18'）中建立压力，所述液压缸能够传递到所述伸缩臂 倾斜装置（16,16'），并且具有后翼（12）克服弹簧力移动到其操作位置的作用。
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