公开(公告)号：US06190905B1

公开(公告)日：2001-02-20

申请号：US09408257

申请日：1999-09-29

Applicant: Henrik Dalbøge ,  Stephan Christgau ,  Lene Nonboe Andersen ,  Lene Venke Kofod ,  Markus Sakari Kauppinen ,  Jack Bech Nielsen ,  Claus Dambmann

Inventor： Henrik Dalbøge ,  Stephan Christgau ,  Lene Nonboe Andersen ,  Lene Venke Kofod ,  Markus Sakari Kauppinen ,  Jack Bech Nielsen ,  Claus Dambmann

IPC: D06M1600

CPC classification number: C11D3/3947 ,  A21D8/042 ,  A23K20/189 ,  C11D3/0078 ,  C11D3/386 ,  C11D3/38618 ,  C11D3/38636 ,  C12N9/62 ,  C12N9/82

Abstract: An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO: 1 or 2. Methods are described for using the protease compositions in reducing vescosity, cleaning contact lenses, baking, and preparing animal feed.

Abstract translation: 在4-7的pH下显示出蛋白酶活性的分离和纯化的酶，其在5％过氧化氢中显示蛋白酶，并且由与SEQ ID NO：1或2所示的DNA序列杂交的DNA序列编码。描述方法 用于使用蛋白酶组合物减少紫外线，清洁隐形眼镜，烘烤和制备动物饲料。

公开(公告)号：US06270968B1

公开(公告)日：2001-08-07

申请号：US09189060

申请日：1998-11-05

Applicant: Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

Inventor： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

IPC: C12Q168

CPC classification number: C12N15/1044 ,  C12N9/2437 ,  C12N9/248 ,  C12Q1/6811 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01032

Abstract: A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.

Abstract translation: 一种用于提供具有感兴趣活性的杂交多肽的方法，其通过以下步骤：i）使用未表征的DNA样品进行PCR扩增和与编码显示所述目的活性的多肽的一种或多种已知基因同源的寡核苷酸引物，以获得一种或多种PCR 产品，ii）将获得的PCR产物连接到5'结构基因序列和3'结构基因序列，其中5'和3'结构基因序列衍生自编码显示所述目标活性的多肽的一个或多个基因， 以形成杂交DNA序列，iii）表达所得的杂交DNA序列，和iv）筛选杂合多肽以鉴定编码显示所述目的活性或相关活性的多肽的序列。

公开(公告)号：US06197564B1

公开(公告)日：2001-03-06

申请号：US09219277

申请日：1998-12-22

Applicant: Lene Venke Kofod ,  MArkus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Henrik Dalbøge ,  Lene Nonboe Andersen ,  Joan Qi Si ,  Tina Sejersgård Jacobsen ,  Niels Munk ,  Anette Müllertz

Inventor： Lene Venke Kofod ,  MArkus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Henrik Dalbøge ,  Lene Nonboe Andersen ,  Joan Qi Si ,  Tina Sejersgård Jacobsen ,  Niels Munk ,  Anette Müllertz

IPC: C12N924

CPC classification number: C12Y302/01032 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L2/04 ,  A23L7/107 ,  A23L29/06 ,  C12C5/004 ,  C12C5/006 ,  C12C7/28 ,  C12G1/00 ,  C12G1/0203 ,  C12N9/2482 ,  C12Y302/01008 ,  D21C5/005

Abstract: A DNA construct encoding an enzyme(s) exhibiting xylanase activity, which enzyme is immunologically reactive with antibody raised against a purified xylanase derived from Aspergillus aculealus, CBS 101.43, recombinant vectors and cells comprising said construct and a method for producing said enzyme using said cell comprising said construct.

Abstract translation: 编码表现出木聚糖酶活性的酶的DNA构建体，该酶与针对源自阿曲曲霉的纯化木聚糖酶，CBS 101.43，重组载体和包含所述构建体的细胞产生的抗体具有免疫反应性，以及使用所述细胞产生所述酶的方法 包括所述构建体。

公开(公告)号：US06265185B1

公开(公告)日：2001-07-24

申请号：US09174768

申请日：1998-10-19

Applicant: Sven Muller ,  Henrik Dalbøge

Inventor： Sven Muller ,  Henrik Dalbøge

IPC: C12P2106

CPC classification number: C12N15/81 ,  C07K14/39 ,  C12N15/815

Abstract: Novel yeast promoters for either EF1-alpha protein or ribosomal protein S7 gene suitable for expression cloning in yeast and heterologous expression of proteins in yeast. The yeast promoters are preferably active in the pH range 4-11 without peptone and obtained from the yeast strain Yarrowia lipolytica.

Abstract translation: 用于酵母中表达克隆的EF1-α蛋白或核糖体蛋白S7基因的新型酵母启动子，酵母中蛋白质的异源表达。 酵母启动子优选在4-11的pH范围内是无活性的，不经蛋白胨从酵母菌株解脂耶氏酵母（Yarrowia lipolytica）获得。

公开(公告)号：US06228630B1

公开(公告)日：2001-05-08

申请号：US09599661

申请日：2000-06-22

Applicant: Lene Venke Kofod ,  MArkus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Henrik Dalbøge ,  Lene Nonboe Andersen ,  Joan Qi Si ,  Tina Sejersgåard Jacobsen ,  Niels Munk ,  Anette Müllertz

Inventor： Lene Venke Kofod ,  MArkus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Henrik Dalbøge ,  Lene Nonboe Andersen ,  Joan Qi Si ,  Tina Sejersgåard Jacobsen ,  Niels Munk ,  Anette Müllertz

IPC: C12N924

CPC classification number: C12Y302/01032 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L2/04 ,  A23L7/107 ,  A23L29/06 ,  C12C5/004 ,  C12C5/006 ,  C12C7/28 ,  C12G1/00 ,  C12G1/0203 ,  C12N9/2482 ,  C12Y302/01008 ,  D21C5/005

Abstract: An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.

Abstract translation: 一种酶制剂，其包含（a）具有木聚糖酶活性的第一酶和（b）具有纤维素分解，木聚糖分解或果胶分解活性的第二酶，并且得到本说明书的支持。