公开(公告)号：US20070178461A1

公开(公告)日：2007-08-02

申请号：US10580415

申请日：2004-11-18

Applicant: Norimasa Miura ,  Goshi Shiota

Inventor： Norimasa Miura ,  Goshi Shiota

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158

Abstract: To provide a cancer diagnostic method capable of detecting evidence presenting the presence of cancer cells in early stage cancer. Cancer diagnostic method comprised of; a process to obtain the sample containing RNA only as a somatic cell and cancer cell fraction from body fluid and a process having a reverse transcription reaction step to generate cDNA using reverse transcriptase from the sample containing said RNA only and a PCR reaction step utilizing fluorescent dye using the following primers for hTERT, CGGAAGAGTGTCTGGAGCAA and GGATGAAGCGGAGTCTGGA to quantify the PCR product amplified by the PCR reaction using the fluorescent dye binding to the PCR product.

Abstract translation: 提供能够检测呈现早期癌症中癌细胞存在的证据的癌症诊断方法。 癌症诊断方法由 获得仅含有体细胞RNA的样品和来自体液的癌细胞级分的样品的方法以及具有逆转录反应步骤的方法以从仅含有所述RNA的样品中使用逆转录酶产生cDNA的方法和利用荧光染料的PCR反应步骤 使用hTERT，CGGAAGAGTGTCTGGAGCAA和GGATGAAGCGGAGTCTGGA的以下引物来定量通过使用与PCR产物结合的荧光染料通过PCR反应扩增的PCR产物。

公开(公告)号：US09555061B2

公开(公告)日：2017-01-31

申请号：US14111757

申请日：2012-04-02

Applicant: Goshi Shiota ,  Yoshiko Hoshikawa ,  Noriko Matsumoto ,  Yoshiaki Matsumi ,  Minoru Morimoto ,  Takayuki Tonoi ,  Hiroyuki Saimoto ,  Kazuo Ohashi ,  Teruo Okano

Inventor： Goshi Shiota ,  Yoshiko Hoshikawa ,  Noriko Matsumoto ,  Yoshiaki Matsumi ,  Minoru Morimoto ,  Takayuki Tonoi ,  Hiroyuki Saimoto ,  Kazuo Ohashi ,  Teruo Okano

IPC: A01N63/00 ,  A61K35/407 ,  C07C251/86 ,  C07C43/225 ,  C07D487/04 ,  C07D307/52 ,  A61L27/38

CPC classification number: A61K35/407 ,  A61L27/3804 ,  A61L27/3834 ,  A61L2430/28 ,  C07C43/225 ,  C07C251/86 ,  C07D307/52 ,  C07D487/04

Abstract: The invention relates to low-molecular-weight compounds which are capable of inducing differentiation of mesenchymal stem cell into hepatocytes.

Abstract translation: 本发明涉及能够诱导间充质干细胞分化为肝细胞的低分子量化合物。

公开(公告)号：US20140112892A1

公开(公告)日：2014-04-24

申请号：US14111757

申请日：2012-04-02

Applicant: Goshi Shiota ,  Yoshiko Hoshikawa ,  Noriko Matsumoto ,  Yoshiaki Matsumi ,  Minoru Morimoto ,  Takayuki Tonoi ,  Hiroyuki Saimoto ,  Kazuo Ohashi ,  Teruo Okano

Inventor： Goshi Shiota ,  Yoshiko Hoshikawa ,  Noriko Matsumoto ,  Yoshiaki Matsumi ,  Minoru Morimoto ,  Takayuki Tonoi ,  Hiroyuki Saimoto ,  Kazuo Ohashi ,  Teruo Okano

IPC: A61K35/407

CPC classification number: A61K35/407 ,  A61L27/3804 ,  A61L27/3834 ,  A61L2430/28 ,  C07C43/225 ,  C07C251/86 ,  C07D307/52 ,  C07D487/04

Abstract: The purpose is to select low-molecular-weight compounds which are effective in inducing differentiation of mesenchymal stem cell into hepatocyte and to develop a safe differentiation-inducing method having excellent efficiency of differentiating mesenchymal stem cell into hepatocyte. Provided are at least one compound selected from the group consisting of compounds represented by formulae (1) and (2), a salt thereof, or a solvate of them; a differentiation inducer comprising at least one compound selected from the group consisting of compounds represented by formulae (1) and (2), a salt thereof, or a solvate of them; and a differentiation inducer comprising a compound represented by formula (8), a salt thereof, or a solvate of them.

Abstract translation: 目的是选择有效诱导间充质干细胞分化为肝细胞的低分子量化合物，并开发出一种安全的分化诱导方法，其具有优良的间充质干细胞分化为肝细胞的效率。 提供至少一种选自由式（1）和（2）表示的化合物，其盐或它们的溶剂化物的化合物; 包含至少一种选自由式（1）和（2）表示的化合物，其盐或它们的溶剂化物的化合物的分化诱导剂; 以及包含由式（8）表示的化合物，其盐或它们的溶剂合物的分化诱导剂。