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1.发明授权公开(公告)号：US12071648B2
公开(公告)日：2024-08-27
申请号：US16650492
申请日：2018-09-25
Applicant: GENOSKIN
Inventor： Claire Jardet , Pascal Descargues , Hanne Norsgaard , Paola Lovato
CPC classification number: C12Q1/025 , C12N5/0629 , C12N2501/15 , C12N2501/2301 , C12N2501/2302 , C12N2501/2323 , C12N2501/51 , C12N2501/515
Abstract: Disclosed is an ex-vivo model of inflamed skin, an in-vitro method for obtaining the same and uses thereof for screening anti-inflammatory compounds. The method includes injecting, into the dermis of a healthy skin biopsy previously taken from a mammal, a composition including an effective amount for activating dermal resident T cells of an anti-CD3 antibody and an anti-CD28 antibody; and b) incubating the injected skin biopsy obtained in step a) in the presence of a composition including an effective amount, for obtaining the polarization of the T cells activated in step a) into LTh1 and/or LTh17 and the synthesis of inflammation markers, of at least one mixture of IL-1β, IL-23, and TGF-β.
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公开(公告)号：US20240410878A1
公开(公告)日：2024-12-12
申请号：US18785910
申请日：2024-07-26
Applicant: GENOSKIN
Inventor： Manon SCHOLAERT , Nicolas GAUDENZIO , Emeline PAGES
IPC: G01N33/50
Abstract: The present invention relates to an in vitro method intended to determine the vaccine potential of a composition comprising the steps of: ia) transcutaneous administration of the composition to a skin explant, comprising the epidermis, dermis and skin appendages as well as a thickness of at least 5 millimeters of hypodermis; ib) determination of the activation status of antigen-presenting cells within the skin explant; and ii) determination of the vaccine potential of the composition.
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公开(公告)号：US20250044277A1
公开(公告)日：2025-02-06
申请号：US18923157
申请日：2024-10-22
Applicant: GENOSKIN
Inventor： Pascal DESCARGUES , Emeline PAGÈS , Claire JARDET
Abstract: The invention relates to an in vitro method for modelling subcutaneous injection comprising the steps of i) mounting, in a cell culture insert, a skin explant comprising a thickness of at least 5 mm of a hypodermis so as to trap the immersed portion of the skin explant with only the epidermis in contact with air, the bottom of which cell insert consisting of a porous membrane, ii) placing the insert in a container or a culture well iii) culturing the skin explant in a medium, and injecting a composition in the hypodermis of the skin explant.
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4.发明申请公开(公告)号：US20240369534A1
公开(公告)日：2024-11-07
申请号：US18778233
申请日：2024-07-19
Applicant: GENOSKIN
Inventor： Claire JARDET , Pascal DESCARGUES , Hanne NORSGAARD , Paola LOVATO
IPC: G01N33/50 , C12N5/00 , C12N5/0783 , G01N1/36
Abstract: The present invention relates to an ex-vivo model of inflamed skin, an in-vitro method for obtaining the same and uses thereof for screening anti-inflammatory compounds.
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5.发明公开公开(公告)号：US20230304996A1
公开(公告)日：2023-09-28
申请号：US18015928
申请日：2021-07-16
Applicant: GENOSKIN
Inventor： Pascal DESCARGUES , Emeline PAGÈS , Laurent MALAQUIN , Emma RAUDE
CPC classification number: G01N33/5082 , C12M21/08 , C12M23/16 , C12M29/10 , C12N5/0698 , C12N2533/54 , C12N2533/76
Abstract: Disclosed is a microfluidic device implanted in an ex-vivo skin explant to control perfusion, diffusion and collection of molecules over long periods. Also disclosed is a method for assessing permeation or infusion of a biomarker of interest through the skin and to a method for detecting and/or quantifying a biomarker of interest contained in the liquid secreted by the ex-vivo skin explant.
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Patent Agency Ranking
公开(公告)号：US12158463B2
公开(公告)日：2024-12-03
申请号：US16971599
申请日：2019-03-03
Applicant: GENOSKIN
Inventor： Pascal Descargues , Emeline Pagès , Claire Jardet
Abstract: Disclosed is an ex vivo model for subcutaneous injection and aims at providing an in vitro method including the steps of i) immersing a skin explant in a solidifiable liquid matrix such that the upper face of the epidermis is not covered, which matrix is itself contained in a cell culture insert, the bottom of which consists of a porous membrane, and (ii) solidifying this matrix so as to trap the immersed portion of this skin explant, wherein the upper face of the epidermis is not covered, and adhering this same matrix to the side walls and the porous membrane of the insert, wherein the skin explant includes a thickness of at least 5 mm of hypodermis.
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7.发明公开公开(公告)号：US20240280565A1
公开(公告)日：2024-08-22
申请号：US18562797
申请日：2022-05-19
Applicant: GENOSKIN
Inventor： Emeline PAGES , Pascal DESCARGUES , Nicolas GAUDENZIO
IPC: G01N33/50
CPC classification number: G01N33/5088
Abstract: This relates to an in vitro method intended to determine the inflammatory potential of a substance including the steps of: ia) administration to a skin explant, topically or by subcutaneous injection, of a composition including the substance; where the skin explant includes the epidermis, the dermis and the skin appendages, as well as a thickness of at least 5 millimeters of hypodermis; ib) determination of the inflammatory response within the skin explant; ic) determination of the level of mast cell degranulation within the skin explant; and ii) determination of the inflammatory potential of the substance and its association or not with an allergic or pseudoallergic potential.
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8.发明申请公开(公告)号：US20200347429A1
公开(公告)日：2020-11-05
申请号：US16650492
申请日：2018-09-25
Applicant: GENOSKIN
Inventor： Claire JARDET , Pascal DESCARGUES , Hanne NORSGAARD , Paola LOVATO
Abstract: Disclosed is an ex-vivo model of inflamed skin, an in-vitro method for obtaining the same and uses thereof for screening anti-inflammatory compounds. The method includes injecting, into the dermis of a healthy skin biopsy previously taken from a mammal, a composition including an effective amount for activating dermal resident T cells of an anti-CD3 antibody and an anti-CD28 antibody; and b) incubating the injected skin biopsy obtained in step a) in the presence of a composition including an effective amount, for obtaining the polarization of the T cells activated in step a) into LTh1 and/or LTh17 and the synthesis of inflammation markers, of at least one mixture of IL-1β, IL-23, and TGF-β.
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9.发明授权System for keeping alive and transporting skin biopsies and applications of said system 有权用于保持活动和运输皮肤活组织检查和所述系统的应用的系统公开(公告)号：US09585381B2
公开(公告)日：2017-03-07
申请号：US14398587
申请日：2013-05-03
Applicant: GENOSKIN
Inventor： Pascal Descargues
CPC classification number: A01N1/0226 , A01N1/0231 , G01N33/502 , G01N33/5044 , G01N33/5082 , G01N2500/10
Abstract: Disclosed is an in vitro or ex vivo method for preserving and/or keeping alive mammalian, preferably human skin biopsies, enabling to transport it and, if applicable, to culture it. Also disclosed is a skin biopsy thus preserved and obtained by such a method and relates to its use as a model especially in a kit for screening or selecting cosmetic or therapeutic compounds.
Abstract translation: 公开了用于保存和/或保持活的哺乳动物，优选人皮肤活组织检查的体外或离体方法，使其能够运输，并且如果适用，培养它。 还公开了通过这种方法保存并获得的皮肤活检，并且涉及其作为模型的用途，特别是用于筛选或选择化妆品或治疗化合物的试剂盒中。
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10.发明申请SYSTEM FOR KEEPING ALIVE AND TRANSPORTING SKIN BIOPSIES AND APPLICATIONS OF SAID SYSTEM 有权用于保持活体和运输皮肤生物和系统的应用的系统公开(公告)号：US20150132737A1
公开(公告)日：2015-05-14
申请号：US14398587
申请日：2013-05-03
Applicant: GENOSKIN
Inventor： Pascal Descargues
CPC classification number: A01N1/0226 , A01N1/0231 , G01N33/502 , G01N33/5044 , G01N33/5082 , G01N2500/10
Abstract: Disclosed is an in vitro or ex vivo method for preserving and/or keeping alive mammalian, preferably human skin biopsies, enabling to transport it and, if applicable, to culture it. Also disclosed is a skin biopsy thus preserved and obtained by such a method and relates to its use as a model especially in a kit for screening or selecting cosmetic or therapeutic compounds.
Abstract translation: 公开了用于保存和/或保持活的哺乳动物，优选人皮肤活组织检查的体外或离体方法，使其能够运输，并且如果适用，培养它。 还公开了通过这种方法保存并获得的皮肤活检，并且涉及其作为模型的用途，特别是用于筛选或选择化妆品或治疗化合物的试剂盒中。
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