The invention relates to an in vitro method for modelling subcutaneous injection comprising the steps of i) mounting, in a cell culture insert, a skin explant comprising a thickness of at least 5 mm of a hypodermis so as to trap the immersed portion of the skin explant with only the epidermis in contact with air, the bottom of which cell insert consisting of a porous membrane, ii) placing the insert in a container or a culture well iii) culturing the skin explant in a medium, and injecting a composition in the hypodermis of the skin explant.