公开(公告)号：US12071648B2

公开(公告)日：2024-08-27

申请号：US16650492

申请日：2018-09-25

Applicant: GENOSKIN

Inventor： Claire Jardet ,  Pascal Descargues ,  Hanne Norsgaard ,  Paola Lovato

IPC: C12Q1/02 ,  C12N5/071

CPC classification number: C12Q1/025 ,  C12N5/0629 ,  C12N2501/15 ,  C12N2501/2301 ,  C12N2501/2302 ,  C12N2501/2323 ,  C12N2501/51 ,  C12N2501/515

Abstract: Disclosed is an ex-vivo model of inflamed skin, an in-vitro method for obtaining the same and uses thereof for screening anti-inflammatory compounds. The method includes injecting, into the dermis of a healthy skin biopsy previously taken from a mammal, a composition including an effective amount for activating dermal resident T cells of an anti-CD3 antibody and an anti-CD28 antibody; and b) incubating the injected skin biopsy obtained in step a) in the presence of a composition including an effective amount, for obtaining the polarization of the T cells activated in step a) into LTh1 and/or LTh17 and the synthesis of inflammation markers, of at least one mixture of IL-1β, IL-23, and TGF-β.