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    Therapeutic fragments of von Willebrand factor
    8.
    发明授权
    Therapeutic fragments of von Willebrand factor 失效
    von Willebrand因子的治疗片段

    公开(公告)号：US5847086A

    公开(公告)日：1998-12-08

    申请号：US487445

    申请日：1995-06-07

    申请人： David L. Farb ,  Michael E. Hrinda ,  Ted C. K. Lee ,  Christopher P. Prior ,  David Weber

    发明人： David L. Farb ,  Michael E. Hrinda ,  Ted C. K. Lee ,  Christopher P. Prior ,  David Weber

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/36 ,  A61P7/02 ,  A61P9/00 ,  A61P43/00 ,  C07K1/22 ,  C07K14/745 ,  C07K14/755 ,  A61K35/14

    CPC分类号： C07K14/755 ,  A61K38/00

    摘要： Processes for preparing aqueous solutions of cysteine-altered von Willebrand Factor fragment which are substantially free of aggregate and capable of therapeutic use for treating thrombosis are provided. The claimed process comprises providing an aqueous solution of vWF fragment and denaturant and containing undesired contaminants, said solution having an acidic pH; separating said contaminants from said solution by contacting said solution with an affinity chromatography medium to which said vWF fragments adhere; eluting said vWF fragment from said affinity chromatography medium in the presence of the denaturant; and separating the eluted fragment from said denaturant while maintaining the aqueous solution of the fragment at a pH of about 2.5 to less than about 5.5 to increase monomerization of, and decrease aggregation of, said fragment, thereby forming an aqueous solution of vWF fragment which is substantially free of aggregate.

    摘要翻译： 提供了制备基本上不含聚集体并能治疗用于治疗血栓形成的半胱氨酸改变的血管性血友病因子片段的水溶液的方法。 所要求的方法包括提供vWF片段和变性剂的水溶液并含有不期望的污染物，所述溶液具有酸性pH; 通过使所述溶液与所述vWF碎片粘附到的亲和层析介质接触来分离所述污染物; 在变性剂存在下，从所述亲和层析介质中洗脱所述vWF片段; 并将洗脱的片段与所述变性剂分离，同时将片段的水溶液保持在约2.5至小于约5.5的pH，以增加所述片段的单体化和降低聚集，由此形成vWF片段的水溶液，其为 基本上不含聚集体。