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1.发明授权Methods of reducing the levels of protoporphyrin IX in recombinant hemoglobin preparations 有权降低重组血红蛋白制剂中原卟啉IX水平的方法
公开(公告)号：US6136565A
公开(公告)日：2000-10-24
申请号：US269584
申请日：1999-10-18
IPC分类号： C12N15/09 , A61K38/00 , C07K14/805 , C12N9/88 , C12N15/60 , C12P21/02 , C12R1/19 , C12P21/04 , C12P21/06
CPC分类号： C12N9/88 , C07K14/805 , A61K38/00
摘要： The present invention relates to recombinant hemoglobin solutions containing reduced amounts of protoporphyrin IX and to methods of producing such hemoglobin solutions. The methods are accomplished by overexpressing the hemH gene in a suitable host cell or by increasing the production of hemH in a host cell, for example, by integration of the hemH gene into the chromosome of the host cell or by inserting multiple copies of the gene in host cells naturally having a hemH gene.
摘要翻译： PCT No.PCT / US97 / 18950 Sec。 371 1999年10月18日第 102（e）1999年10月18日PCT PCT 1997年10月17日PCT公布。 公开号WO98 / 17809 日期：1998年04月30日本发明涉及含有减少量的原卟啉IX的重组血红蛋白溶液以及生产这种血红蛋白溶液的方法。 该方法通过在合适的宿主细胞中过表达hemH基因或通过增加宿主细胞中hemH的产生来实现，例如通过将hemH基因整合到宿主细胞的染色体中或通过插入多个拷贝的基因 在天然具有hemH基因的宿主细胞中。
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公开(公告)号：US07655627B2
公开(公告)日：2010-02-02
申请号：US11718636
申请日：2005-12-07
摘要： The present invention relates to novel muteins of human fibroblast growth factor 21 with reduced deamidation compared to wild-type human FGF-21. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, or metabolic syndrome.
摘要翻译： 本发明涉及与野生型人FGF-21相比，减少脱酰胺的人成纤维细胞生长因子21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症或代谢综合征的方法。
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公开(公告)号：US20090118190A1
公开(公告)日：2009-05-07
申请号：US12349119
申请日：2009-01-06
申请人： John Michael Beals , Christopher Carl Frye , Wolfgang Glaesner , Shun Li , Radhakrishnan Rathnachalam , Jing Shang , Beth Ann Strifler , Radmila Micanovic
发明人： John Michael Beals , Christopher Carl Frye , Wolfgang Glaesner , Shun Li , Radhakrishnan Rathnachalam , Jing Shang , Beth Ann Strifler , Radmila Micanovic
摘要： The present invention relates to novel muteins of human fibroblast growth factor 21 with improved pharmaceutical properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, metabolic syndrome, and in reducing the mortality and morbidity of critically ill patients.
摘要翻译： 本发明涉及具有改善的药物性质的人成纤维细胞生长因子21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症，代谢综合征以及降低危重病人死亡率和发病率的方法。
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公开(公告)号：US6008041A
公开(公告)日：1999-12-28
申请号：US850227
申请日：1997-05-02
摘要： The present invention provides the cDNA sequence encoding bovine dipeptidylaminopeptidase 1. The invention demonstrates that bovine DAP 1 coded by a single cDNA. The mature protein is derived from a single polypeptide consisting of a signal peptide, and a major polypeptide which is processed to generate the a subunit, b subunit and g subunit.
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公开(公告)号：US07622445B2
公开(公告)日：2009-11-24
申请号：US11571933
申请日：2005-08-25
摘要： The present invention relates to novel muteins of human fibroblast growth factor-21 with reduced susceptibility for proteolytic degradation when expressed in yeast. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, or metabolic syndrome.
摘要翻译： 本发明涉及当在酵母中表达时具有降低的蛋白水解降解易感性的人成纤维细胞生长因子-21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症或代谢综合征的方法。
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公开(公告)号：US07582607B2
公开(公告)日：2009-09-01
申请号：US11574332
申请日：2005-07-26
摘要： The present invention relates to novel muteins of human fibroblast growth factor 21 with reduced capacity of O-glycosylation when expressed in yeast compared to wild-type human FGF-21. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, or metabolic syndrome.
摘要翻译： 本发明涉及当与野生型人FGF-21相比时在酵母中表达时O-糖基化能力降低的人成纤维细胞生长因子21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症或代谢综合征的方法。
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公开(公告)号：US20080103096A1
公开(公告)日：2008-05-01
申请号：US11574332
申请日：2005-07-26
IPC分类号： A61K38/18
摘要： The present invention relates to novel muteins of human fibroblast growth factor 21 with reduced capacity of O-glycosylation when expressed in yeast compared to wild-type human FGF-21. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, or metabolic syndrome.
摘要翻译： 本发明涉及当与野生型人FGF-21相比时在酵母中表达时O-糖基化能力降低的人成纤维细胞生长因子21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症或代谢综合征的方法。
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公开(公告)号：US07491697B2
公开(公告)日：2009-02-17
申请号：US10579510
申请日：2004-12-01
申请人： John Michael Beals , Christopher Carl Frye , Wolfgang Glaesner , Shun Li , Radhakrishnan Rathnachalam , Jing Shang , Beth Ann Strifler , Radmila Micanovic
发明人： John Michael Beals , Christopher Carl Frye , Wolfgang Glaesner , Shun Li , Radhakrishnan Rathnachalam , Jing Shang , Beth Ann Strifler , Radmila Micanovic
摘要： The present invention relates to novel muteins of human fibroblast growth factor 21 with improved pharmaceutical properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, metabolic syndrome, and in reducing the mortality and morbidity of critically ill patients.
摘要翻译： 本发明涉及具有改善的药物性质的人成纤维细胞生长因子21的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了治疗2型糖尿病，肥胖症，代谢综合征以及降低危重病人死亡率和发病率的方法。
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公开(公告)号：US6027911A
公开(公告)日：2000-02-22
申请号：US54985
申请日：1998-04-03
摘要： The present invention provides the cDNA sequence encoding bovine dipeptidylaminopeptidase 1. The invention demonstrates that bovine DAP 1 coded by a single cDNA. The mature protein is derived from a single polypeptide consisting of a signal peptide, and a major polypeptide which is processed to generate the a subunit, b subunit and g subunit.
摘要翻译： 本发明提供了编码牛二肽基氨基肽酶1的cDNA序列。本发明证明了通过单个cDNA编码的牛DAP1。 成熟蛋白质衍生自由信号肽组成的单一多肽和被处理产生亚基，b亚基和g亚基的主要多肽。
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