公开(公告)号：US08679829B2

公开(公告)日：2014-03-25

申请号：US10559583

申请日：2004-06-02

Applicant: Christa Schleper ,  Melanie Jonuscheit ,  Jürgen Eck ,  Frank Niehaus ,  Sonja-Verena Albers ,  Sabrina Froels

Inventor： Christa Schleper ,  Melanie Jonuscheit ,  Jürgen Eck ,  Frank Niehaus ,  Sonja-Verena Albers ,  Sabrina Froels

IPC: C12N15/00 ,  C12N15/866 ,  C12N15/70 ,  C12N15/11

CPC classification number: C12N15/74

Abstract: The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.

Abstract translation: 本发明涉及一种磺叶病毒表达载体，其包含：（a）磺基梭菌复制起点; （b）编码与表达控制序列和包装信号有效连接的结构蛋白和SSV1，SSV2或pSSVx的位点特异性整合酶的基因; （c）一个或多个可选择的标记基因，其可操作地连接到子宫颈表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'端的磺基叶绿体启动子。 此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中扩增和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制的大肠杆菌; 和（b）用于在大肠杆菌中选择的标记物。 此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。 最后，本申请还涉及产生感染性亚病毒颗粒的方法。

公开(公告)号：US20070134201A1

公开(公告)日：2007-06-14

申请号：US10559583

申请日：2004-06-02

Applicant: Christa Schleper

Inventor： Christa Schleper

IPC: A61K48/00 ,  C07H21/04 ,  C07K14/245 ,  C12N1/21 ,  C12N15/74 ,  C12N7/00

CPC classification number: C12N15/74

Abstract: The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.

Abstract translation: 本发明涉及一种磺叶病毒表达载体，其包含：（a）磺基梭菌复制起点; （b）编码与表达控制序列和包装信号有效连接的结构蛋白和SSV1，SSV2或pSSVx的位点特异性整合酶的基因; （c）一个或多个可选择的标记基因，其可操作地连接到子宫颈表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'端的磺基叶绿体启动子。 此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中扩增和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制的大肠杆菌; 和（b）用于在大肠杆菌中选择的标记物。 此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。 最后，本申请还涉及产生感染性亚病毒颗粒的方法。

公开(公告)号：US06632937B1

公开(公告)日：2003-10-14

申请号：US09408020

申请日：1999-09-29

Applicant: Ronald V. Swanson ,  Robert A. Feldman ,  Christa Schleper

Inventor： Ronald V. Swanson ,  Robert A. Feldman ,  Christa Schleper

IPC: C07H204

CPC classification number: C07K14/195

Abstract: The present application relates to nucleic acids and polypeptides from Cenarchaeum symbiosum. Methods of making the polypeptides and antibodies against the polypeptides are also described.

Abstract translation: 本申请涉及来自 共生共生体的核酸和多肽。 还描述了制备针对多肽的多肽和抗体的方法。

公开(公告)号：US20060240423A1

公开(公告)日：2006-10-26

申请号：US10525708

申请日：2003-08-20

Applicant: Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jurgen Eck

Inventor： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jurgen Eck

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C12N15/1003

Abstract: The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

Abstract translation: 本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子 尺寸。 此外，本发明涉及用于分离核酸分子的方法，包括将样品施用于本发明的装置，其中所述核酸分子优选代表给定栖息地的元素的一部分。 此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。

公开(公告)号：US07749366B2

公开(公告)日：2010-07-06

申请号：US10525708

申请日：2003-08-20

Applicant: Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander H. Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jürgen Eck

Inventor： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander H. Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jürgen Eck

IPC: G01N33/559 ,  C40B60/10 ,  C12M1/00

CPC classification number: C12N15/1003

Abstract: The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

Abstract translation: 本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子 尺寸。 此外，本发明涉及用于分离核酸分子的方法，包括将样品施用于本发明的装置，其中所述核酸分子优选代表给定栖息地的元素的一部分。 此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。