知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.042 秒)

    ENGINEERED FLUORESCENT SPONTANEOUS ISOMERIZATION RATE BIOSENSORS
    1.
    发明公开
    ENGINEERED FLUORESCENT SPONTANEOUS ISOMERIZATION RATE BIOSENSORS 审中-公开

    公开(公告)号：US20240353414A1

    公开(公告)日：2024-10-24

    申请号：US18684651

    申请日：2022-08-18

    申请人： Calico Life Sciences LLC

    发明人： Maria Ingaramo

    IPC分类号： G01N33/68 ,  G01N33/58

    CPC分类号： G01N33/68 ,  G01N33/582

    摘要： Described herein are countdown biosensors, and methods of using the same, comprising fluorophores which can spontaneously photoswitch between two or more states with different fluorescent properties (e.g. fluorescent intensity or fluorescent color). The countdown sensor comprises a fluorescent domain which can spontaneously photoswitch, and a sensing domain which responds to the desired input. The countdown sensor is “read” by measuring the photoswitching rate. In certain embodiments, the decay of fluorescent intensity over time (due to spontaneous photoswitching of different fluorescent domains) can be made to depend on the concentration of different small molecules, such as hydrogen ion.

    System and method for inferring protein binding
    10.
    发明授权
    System and method for inferring protein binding 有权

    公开(公告)号：US11709136B2

    公开(公告)日：2023-07-25

    申请号：US17253444

    申请日：2019-06-17

    申请人： CALICO LIFE SCIENCES LLC

    发明人： Andrew Gregory York ,  Maria Del Mar Ingaramo

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6408 ,  G01N21/6445

    摘要： Methods and apparatus for inferring protein binding based on rotational diffusion of a collection of fluorophores. One example of a method includes applying a first light pulse to excite a plurality of fluorophores in the collection of fluorophores to produce a plurality of excited fluorophores, the first light pulse having a first polarization and the plurality of excited fluorophores having a component of their orientation aligned with the first polarization, applying a second light pulse to stimulate emission by the plurality of excited fluorophores, the second light pulse having a second polarization orthogonal to the first polarization, after a time delay following application of the second light pulse, applying a third light pulse of the second polarization to further stimulate emission by the plurality of excited fluorophores, detecting polarized emissions from the plurality of excited fluorophores, and inferring the rate of rotational diffusion of the collection of fluorophores based on the detected polarized emissions.