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1.发明授权公开(公告)号：US07906317B2
公开(公告)日：2011-03-15
申请号：US11875940
申请日：2007-10-21
Applicant: Bor-Heng Lee , Chiu-Cho Yan , Ying-Chih Wang , Ching-Yu Lin
Inventor： Bor-Heng Lee , Chiu-Cho Yan , Ying-Chih Wang , Ching-Yu Lin
CPC classification number: A61B10/0045 , A61B10/0096 , A61B2010/0074 , Y10S435/803 , Y10S435/805
Abstract: The present invention discloses an apparatus for thin-layer cell smear preparation and in-situ hybridization, comprising at least one positioning device and a sealing device. The positioning device comprises at least one first opening. When the positioning device is set on a carrier device, the wall of the first opening and the carrier device form a cavity. The cavity is used to accommodate a cell suspension. The sealing device is provided on the positioning device for sealing the cavity to form an enclosed space.
Abstract translation: 本发明公开了一种用于薄层细胞涂片制备和原位杂交的装置，包括至少一个定位装置和密封装置。 定位装置包括至少一个第一开口。 当定位装置设置在载体装置上时，第一开口的壁和载体装置形成空腔。 空腔用于容纳细胞悬浮液。 密封装置设置在定位装置上，用于密封空腔以形成封闭空间。
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2.发明申请公开(公告)号：US20070031827A1
公开(公告)日：2007-02-08
申请号：US11520354
申请日：2006-09-12
Applicant: Ching-Yu Lin , Ruey-Wen Lin , Chiou-Mien You , Hsing-Hsuan Huang , Bor-Heng Lee , Hsien-Hsiung Lee , Yu-Ju Lin , Chih-Chun Fan , Han-Chuan Hsu , Chia-Wen Shih , Chih-Hsing Yeh , Yi-Feng Kao , Chih-Long Pan , Peter Chan
Inventor： Ching-Yu Lin , Ruey-Wen Lin , Chiou-Mien You , Hsing-Hsuan Huang , Bor-Heng Lee , Hsien-Hsiung Lee , Yu-Ju Lin , Chih-Chun Fan , Han-Chuan Hsu , Chia-Wen Shih , Chih-Hsing Yeh , Yi-Feng Kao , Chih-Long Pan , Peter Chan
CPC classification number: C12Q1/708 , C12Q2565/501
Abstract: A detector for detecting and simultaneously diagnosing at least one subtype of human papilloma viruses (HPV) contained in a biological sample is provided. The detector comprises: a carrier, a plurality of micro-dots immobilized on the carrier, wherein each micro-dot is for identifying one particular HPV subtype, and the HPV subtype is one selected from a group consisting of 39 different HPV subtypes; and at least one oligonucleotide sequence contained in each the micro-dot that is specific to the one particular HPV subtype, wherein the at least one oligonucleotide sequence serves as a detection probe that hybridizes specifically with an L1 gene sequence of the one particular HPV subtype to form a hybridization complex as a detection indicator, so that each micro-dot identifies one particular HPV subtype via a corresponding oligonucleotide of the one particular HPV subtype, and thereby detecting and simultaneously identifying subtypes of human papilloma viruses.
Abstract translation: 提供了用于检测并同时诊断生物样品中包含的至少一种人乳头瘤病毒（HPV）亚型的检测器。 检测器包括：载体，固定在载体上的多个微点，其中每个微点用于鉴定一个特定的HPV亚型，并且HPV亚型是从39个不同的HPV亚型组成的组中选择的一个; 以及至少一个寡核苷酸序列，其特征在于每个特定HPV亚型的微点中包含的寡核苷酸序列，其中所述至少一个寡核苷酸序列用作与所述特定HPV亚型的L1基因序列特异性杂交的检测探针， 形成杂交复合物作为检测指标，使得每个微点通过一个特定HPV亚型的相应寡核苷酸识别一个特定的HPV亚型，从而检测并同时鉴定人乳头状瘤病毒的亚型。
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3.发明申请公开(公告)号：US20090104686A1
公开(公告)日：2009-04-23
申请号：US11875940
申请日：2007-10-21
Applicant: Bor-Heng Lee , Chiu-Cho Yan , Ying-Chih Wang , Ching-Yu Lin
Inventor： Bor-Heng Lee , Chiu-Cho Yan , Ying-Chih Wang , Ching-Yu Lin
IPC: C12M1/00
CPC classification number: A61B10/0045 , A61B10/0096 , A61B2010/0074 , Y10S435/803 , Y10S435/805
Abstract: The present invention discloses an apparatus for thin-layer cell smear preparation and in-situ hybridization, comprising at least one positioning device and a sealing device. The positioning device comprises at least one first opening. When the positioning device is set on a carrier device, the wall of the first opening and the carrier device form a cavity. The cavity is used to accommodate a cell suspension. The sealing device is provided on the positioning device for sealing the cavity to form an enclosed space.
Abstract translation: 本发明公开了一种用于薄层细胞涂片制备和原位杂交的装置，包括至少一个定位装置和密封装置。 定位装置包括至少一个第一开口。 当定位装置设置在载体装置上时，第一开口的壁和载体装置形成空腔。 空腔用于容纳细胞悬浮液。 密封装置设置在定位装置上，用于密封空腔以形成封闭空间。
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4.发明申请公开(公告)号：US20050175989A1
公开(公告)日：2005-08-11
申请号：US10601497
申请日：2003-06-23
Applicant: Ching-Yu Lin , Ruey-Wen Lin , Chiou-Mien You , Chiu-Cho Yan , Bor-Heng Lee , Hsien-Hsiung Lee , Yu-Ju Lin , Chih-Chun Fan , Han-Chuan Hsu , Chia-Wen Shih , Chih-Hsing Yeh , Yi-Feng Kao , Chih-Long Pan , Yen-Ming Lin , Peter Chan
Inventor： Ching-Yu Lin , Ruey-Wen Lin , Chiou-Mien You , Chiu-Cho Yan , Bor-Heng Lee , Hsien-Hsiung Lee , Yu-Ju Lin , Chih-Chun Fan , Han-Chuan Hsu , Chia-Wen Shih , Chih-Hsing Yeh , Yi-Feng Kao , Chih-Long Pan , Yen-Ming Lin , Peter Chan
CPC classification number: C12Q1/708 , C12Q2565/501
Abstract: A detector for detecting and simultaneously diagnosing at least one subtype of human papilloma viruses (HPV) contained in a biological sample is provided. The detector comprises: a carrier, a plurality of micro-dots immobilized on the carrier, wherein each micro-dot is for identifying one particular HPV subtype, and the HPV subtype is one selected from a group consisting of 39 different HPV subtypes; and at least one oligonucleotide sequence contained in each the micro-dot that is specific to the one particular HPV subtype, wherein the at least one oligonucleotide sequence serves as a detection probe that hybridizes specifically with an L1 gene sequence of the one particular HPV subtype to form a hybridization complex as a detection indicator, so that each micro-dot identifies one particular HPV subtype via a corresponding oligonucleotide of the one particular HPV subtype, and thereby detecting and simultaneously identifying subtypes of human papilloma viruses.
Abstract translation: 提供了用于检测并同时诊断生物样品中包含的至少一种人乳头瘤病毒（HPV）亚型的检测器。 检测器包括：载体，固定在载体上的多个微点，其中每个微点用于鉴定一个特定的HPV亚型，并且HPV亚型是从39个不同的HPV亚型组成的组中选择的一个; 以及至少一个寡核苷酸序列，其特征在于每个特定HPV亚型的微点中包含的寡核苷酸序列，其中所述至少一个寡核苷酸序列用作与所述特定HPV亚型的L1基因序列特异性杂交的检测探针， 形成杂交复合物作为检测指标，使得每个微点通过一个特定HPV亚型的相应寡核苷酸识别一个特定的HPV亚型，从而检测并同时鉴定人乳头状瘤病毒的亚型。
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