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公开(公告)号：US20190218568A1
公开(公告)日：2019-07-18
申请号：US16371837
申请日：2019-04-01
发明人： Petra CIRPUS , Jörg BAUER , Xiao QIU , Guohai WU , Bifang CHENG , Martin TRUKSA , Tom WETJEN
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
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公开(公告)号：US10308914B2
公开(公告)日：2019-06-04
申请号：US15175278
申请日：2016-06-07
发明人： Jörg Bauer , Tom Wetjen
IPC分类号： A23D9/00 , A23D9/02 , C12N9/00 , C12N9/02 , C12N9/10 , C12P7/64 , A23K50/80 , A61K38/00 , C12N15/82 , A23K20/158 , A23L33/105 , A23L33/115
摘要： The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.
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3.发明申请PLANTS WITH INCREASED TOLERANCE AND/OR RESISTANCE TO ENVIRONMENTAL STRESS AND INCREASED BIOMASS PRODUCTION 审中-公开具有增加的耐受性和/或抵抗环境压力和增加生物量生产的植物公开(公告)号：US20160201077A1
公开(公告)日：2016-07-14
申请号：US15047937
申请日：2016-02-19
发明人： Piotr Puzio , Oliver BLÄSING , Oliver Thimm
IPC分类号： C12N15/82
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8261 , C12N15/8273
摘要： This invention relates generally to a plant cell with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of polypeptides associated with abiotic stress responses and abiotic stress tolerance in plants.
摘要翻译： 本发明一般涉及通过增加或产生与非生物胁迫反应相关的多肽的一种或多种活性而与相应的未转化的野生型植物细胞相比具有增加的耐受性和/或耐环境胁迫和增加的生物量产生的植物细胞，以及 植物中非生物胁迫耐受性。
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公开(公告)号：US20150152432A1
公开(公告)日：2015-06-04
申请号：US14615849
申请日：2015-02-06
发明人： Hardy Schön , Oliver Thimm , Gerhard Ritte , Oliver Bläsing , Koen Bruynseels , Yves Hatzfeld , Valerie Frankard , Ana Isabel Sanz Molinero , Christophe Reuzeau , Steven Vandenabeele
IPC分类号： C12N15/82
CPC分类号： C12N15/8273 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a method for producing a plant with increased yield as compared to a corresponding wild type plant comprising increasing or generating one or more protein activities in a plant or a part thereof. The present invention further relates to nucleic acids enhancing or improving one or more traits of a transgenic plant, and cells, progenies, seeds and pollen derived from such plants or parts, as well as methods of making and methods of using such plant cell(s) or plant(s), progenies, seed(s) or pollen. Particularly, the improved trait(s) are manifested as increased yield, preferably by improving one or more yield-related trait(s).
摘要翻译： 本发明提供了与相应的野生型植物相比生产具有增加的产量的植物的方法，包括在植物或其部分中增加或产生一种或多种蛋白质活性。 本发明还涉及增强或改善一种或多种转基因植物，以及衍生自这种植物或部分的细胞，后代，种子和花粉的一种或多种性状的核酸，以及制备方法和使用这种植物细胞的方法 ）或植物，后代，种子或花粉。 特别地，改善的性状表现为增加的产量，优选通过改善一种或多种产量相关性状。
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5.发明申请Plants Having Increased Yield-Related Traits and a Method for Making the Same 审中-公开增加产量相关性状的植物及其制作方法公开(公告)号：US20140165230A1
公开(公告)日：2014-06-12
申请号：US14182772
申请日：2014-02-18
发明人： Yves Hatzfeld , Valerie Frankard
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： The present invention concerns a method for increasing yield-related traits by modulating expression in a plant of a nucleic acid encoding an AT-hook motif nuclear localized 19/20 (AHL19/20) polypeptide, a Growth Regulating Protein (GRP) polypeptide which is a metallothionein 2a (MT2a) polypeptide, or an alanine aminotransferase (AAT) polypeptide. Also provided are plants having increased yield-related traits relative to control plants thus obtained and constructs useful in the methods of the invention.
摘要翻译： 本发明涉及通过调节植物中编码AT-钩基序核定位的19/20（AHL19 / 20）多肽，生长调节蛋白（GRP）多肽的核酸表达来增加产量相关性状的方法， 金属硫蛋白2a（MT2a）多肽或丙氨酸氨基转移酶（AAT）多肽。 还提供了相对于如此获得的对照植物具有增加的产量相关性状的植物和可用于本发明方法的植物。
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6.发明授权Use of Armadillo repeat (ARM1) polynucleotides for obtaining pathogen resistance in plants 有权使用犰狳重复（ARM1）多核苷酸获得植物中的病原体抗性公开(公告)号：US08735654B2
公开(公告)日：2014-05-27
申请号：US13689008
申请日：2012-11-29
发明人： Dimitar Douchkov , Patrick Schweizer , Uwe Zierold
CPC分类号： C12N15/8279 , C12N15/8282
摘要： The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one Armadillo repeat polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare Armadillo repeat (HvARM) polynucleotide and describes homologous sequences (ARM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants e, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.
摘要翻译： 本发明涉及通过减少至少一种犰狳重复多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦禾本科犰狳重复（HvARM）多核苷酸的新型核酸序列，并描述其同源序列（ARM1）及其在植物中获得病原体抗性的方法和用于核酸构建体，表达 盒和载体，其包含这些序列并且适于介导植物中的真菌抗性。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。
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公开(公告)号：US20040194163A1
公开(公告)日：2004-09-30
申请号：US10688481
申请日：2003-10-17
IPC分类号： A01H001/00 , C12N015/82
CPC分类号： C12N15/8273 , A01K2217/05 , C07K14/415 , C12N9/1205 , C12N9/16 , C12N15/8217
摘要： A transgenic plant transformed by a GTP Binding Stress-Related Protein (GBSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated GBSRPs, and isolated nucleic acid coding GBSRPs, and vectors and host cells containing the latter.
摘要翻译： 通过GTP结合应激相关蛋白（GBSRP）编码核酸转化的转基因植物，其中植物中核酸序列的表达与植物的野生型品种相比，导致对环境胁迫的耐受性增加。 还提供了由转基因植物生产的农产品，包括种子。 还提供了分离的GBSRP和分离的编码GBSRP的核酸，以及含有后者的载体和宿主细胞。
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公开(公告)号：US11168308B2
公开(公告)日：2021-11-09
申请号：US16379935
申请日：2019-04-10
发明人： Jörg Bauer , Tom Wetjen
IPC分类号： C12N9/02 , A23L33/105 , A23L33/115 , A23K20/158 , A23K50/80 , A23D9/00 , C12N9/10 , C12N15/82 , C12P7/64 , C12N9/00 , A23D9/02 , A61K38/00
摘要： The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.
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公开(公告)号：US20210163975A1
公开(公告)日：2021-06-03
申请号：US17085135
申请日：2020-10-30
发明人： Petra Cirpus , Joerg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
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公开(公告)号：US10676752B2
公开(公告)日：2020-06-09
申请号：US14737789
申请日：2015-06-12
发明人： Peifeng Ren , Hee-Sook Song , John McMillan
摘要： The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding such microRNAs. By incorporation of the microRNA encoding sequence the expression from the expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding the microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.
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