公开(公告)号：US20070268171A1

公开(公告)日：2007-11-22

申请号：US11412562

申请日：2006-04-27

申请人： August Hidalgo ,  John Fjeldsted ,  William Frazer ,  Carl Myerholtz

发明人： August Hidalgo ,  John Fjeldsted ,  William Frazer ,  Carl Myerholtz

IPC分类号： H03M1/12

CPC分类号： H03M1/188 ,  H01J49/0031

摘要： A mass spectrometer comprises an ion detector, a first amplifier, a second amplifier, and a spectra combiner. The ion detector is configured to generate an analog signal indicative of ions detected by the ion detector. The first amplifier is configured to amplify the analog signal to provide a first amplified signal having a first gain relative to the analog signal. The second amplifier is configured to amplify the analog signal to provide a second amplified signal having a second gain relative to the analog signal, and the first gain is different than the second gain. The spectra combiner is configured to combine first summed digital samples of the first amplified signal with second summed digital samples of the second amplified signal.

摘要翻译： 质谱仪包括离子检测器，第一放大器，第二放大器和光谱合成器。 离子检测器被配置为产生指示由离子检测器检测到的离子的模拟信号。 第一放大器被配置为放大模拟信号以提供相对于模拟信号具有第一增益的第一放大信号。 第二放大器被配置为放大模拟信号以提供相对于模拟信号具有第二增益的第二放大信号，并且第一增益不同于第二增益。 频谱组合器被配置为将第一放大信号的第一相加数字采样与第二放大信号的第二相加数字采样相组合。

公开(公告)号：US20050069905A1

公开(公告)日：2005-03-31

申请号：US10676957

申请日：2003-09-30

申请人： Carl Myerholtz ,  May Tom-Moy ,  August Hidalgo ,  William McAllister

发明人： Carl Myerholtz ,  May Tom-Moy ,  August Hidalgo ,  William McAllister

IPC分类号： C12Q1/68 ,  G01N33/543 ,  C12M1/34

CPC分类号： G01N33/5438 ,  C12Q1/6825

摘要： A device for electronic detection of a target includes a probe, e.g. an oligonucleotide, attached to a pad of resistive material, wherein the pad is adjacent a first electrode and also is adjacent a second electrode. In use, the probe is contacted with a sample containing the target, e.g. a target nucleic acid, under conditions and for a time sufficient to allow target to bind the probe. An enhancement reaction is then applied to result in a change in an observable property of the device. The observable property is then monitored using measurement apparatus operably associated with the device. Typically, multiple devices will be present on an array of devices, allowing multiplex analysis of multiple different targets using a single array of devices.

摘要翻译： 用于电子检测靶的装置包括探针，例如。 附着到电阻材料焊盘的寡核苷酸，其中所述焊盘与第一电极相邻并且还邻近第二电极。 在使用中，探针与含有靶标的样品接触。 在足以允许靶结合探针的条件下和足够时间的靶核酸。 然后应用增强反应以导致装置的可观察性质的变化。 然后使用与装置可操作地相关联的测量装置监测可观察的性质。 通常，多个设备将存在于设备阵列上，允许使用单个阵列阵列对多个不同目标进行多路复用分析。

公开(公告)号：US20110024620A1

公开(公告)日：2011-02-03

申请号：US12511965

申请日：2009-07-29

申请人： August Hidalgo ,  Brian D. Setterberg ,  George Stennis Moore ,  Michael Manlove

发明人： August Hidalgo ,  Brian D. Setterberg ,  George Stennis Moore ,  Michael Manlove

IPC分类号： H01J49/40

CPC分类号： H01J49/40 ,  H01J49/0027

摘要： A detection signal generated in response to incident ions accelerated at temporally-irregular intervals having an average repetition rate greater than a reference repetition rate represents detection events each having an event time and an intensity. For each detection event, respective allowed TOFs between the event time and the transient times are calculated. Using respective initial probabilities, initial apportionments of the intensity of each detection event among the allowed TOFs linked thereto are determined. For each allowed TOF, the intensity apportionments thereto are accumulated to generate an intensity accumulation linked thereto. For each detection event, respective revised probabilities are iteratively determined using the intensity accumulations linked to the allowed TOFs linked thereto, and the respective intensity is iteratively reapportioned among the allowed TOFs linked thereto using the revised probabilities to transform the detection signal to a time-of-flight spectrum.

摘要翻译： 响应于以大于参考重复频率的平均重复频率在时间不规则的间隔加速的入射离子产生的检测信号表示每个具有事件时间和强度的检测事件。 对于每个检测事件，计算事件时间和瞬时时间之间的相应允许的TOF。 使用各自的初始概率，确定与其相关联的允许的TOF中的每个检测事件的强度的初始分配。 对于每个允许的TOF，累积其强度分配以产生与其相关联的强度累积。 对于每个检测事件，使用链接到与其链接的允许的TOF的强度累积来迭代地确定相应的修订概率，并且使用修订的概率在相关联的其中允许的TOF之间迭代地重新分配相应的强度，以将检测信号变换为 - 光谱。

公开(公告)号：US07723680B2

公开(公告)日：2010-05-25

申请号：US11897693

申请日：2007-08-31

申请人： August Hidalgo ,  Kenneth L Staton

发明人： August Hidalgo ,  Kenneth L Staton

IPC分类号： H01J40/00

CPC分类号： H01J43/30 ,  H01J43/26 ,  H01J49/025

摘要： A system for detecting ions is disclosed. The system includes a detector having a plurality of dynodes arranged in an electron cascading configuration, and a power supply circuit electrically coupled to the plurality of dynodes. The plurality of dynodes include a first dynode and a second dynode. The power supply circuit is arranged to selectively adjust a potential difference between the first and second dynodes between a detection mode and a blanking mode. A method of detecting ions is also disclosed.

摘要翻译： 公开了一种用于检测离子的系统。 该系统包括具有以电子级联配置布置的多个倍增电极的检测器和电耦合到多个倍增电极的电源电路。 多个倍增电极包括第一倍增电极和第二倍增电极。 电源电路设置成在检测模式和消隐模式之间选择性地调节第一和第二倍增电极之间的电位差。 还公开了一种检测离子的方法。

公开(公告)号：US20070255532A1

公开(公告)日：2007-11-01

申请号：US11412887

申请日：2006-04-27

申请人： John Fjeldsted ,  August Hidalgo ,  William Frazer

发明人： John Fjeldsted ,  August Hidalgo ,  William Frazer

IPC分类号： G06F19/00 ,  G06F17/40

CPC分类号： H01J49/0036

摘要： A mass spectrometer comprises an ion detector, an analog-to-digital (A/D) converter, a sample adjuster, and an adder. The A/D converter is configured to receive and sample an analog signal from the ion detector thereby providing a plurality of samples. The adder is configured to sum the samples, and the summed samples define a mass spectrum. The sample adjuster is configured to identify a peak defined by the samples and to suppress at least one of the samples of the peak such that a resolution of a peak within the mass spectrum is enhanced.

摘要翻译： 质谱仪包括离子检测器，模拟数字（A / D）转换器，样本调节器和加法器。 A / D转换器被配置为从离子检测器接收和采样模拟信号，从而提供多个样本。 加法器被配置为对样本求和，并且求和的样本定义质谱。 样本调整器被配置为识别由样本限定的峰值并且抑制峰值的至少一个样本，使得质谱图中的峰的分辨率增强。

公开(公告)号：US07208726B2

公开(公告)日：2007-04-24

申请号：US10927874

申请日：2004-08-27

申请人： August Hidalgo ,  Stuart C. Hansen ,  Gangqiang Li

发明人： August Hidalgo ,  Stuart C. Hansen ,  Gangqiang Li

IPC分类号： H01J49/00 ,  H01J49/40

CPC分类号： H01J49/401 ,  H01J49/422 ,  H01J49/424

摘要： An apparatus for analyzing ions is described. The apparatus includes an ion source, an ion trap positioned to receive ions from the ion source; a time of flight mass analyzer, and a detector operatively coupled to the time of flight. The time of flight mass analyzer includes a pulser region, and the pulser region is positioned to receive ions from the ion trap. The apparatus further includes a scanning delay timing circuit in operable relation to the pulser region. The scanning delay timing circuit is adapted to triggering an extraction pulse at the pulser region. Methods of analyzing ions by mass spectrometry are also described.

摘要翻译： 描述了用于分析离子的装置。 该装置包括离子源，定位成从离子源接收离子的离子阱; 飞行时间质量分析器，以及可操作地耦合到飞行时间的检测器。 飞行时间质量分析器包括脉冲发生器区域，并且脉冲发生器区域被定位成从离子阱接收离子。 该装置还包括与脉冲发生器区域可操作的扫描延迟定时电路。 扫描延迟定时电路适于在脉冲发生器区域触发提取脉冲。 还描述了通过质谱分析离子的方法。

公开(公告)号：US06822227B1

公开(公告)日：2004-11-23

申请号：US10628953

申请日：2003-07-28

申请人： August Hidalgo ,  Ken Poulton

发明人： August Hidalgo ,  Ken Poulton

IPC分类号： H01J4940

CPC分类号： H01J49/40 ,  H01J49/025

摘要： A mass spectrometer having an ion accelerator and an ion detector utilizing a finite impulse response filter is disclosed. The ion accelerator generates an ion pulse in response to a start signal. A clock increments a register that indicates the time that has elapsed since the start signal. The ion detector is spatially separated from the ion accelerator and generates a measurement signal indicative of ions striking the detector. The measurement signal is filtered through a finite impulse response filter to form a filtered measurement signal. The finite impulse response filter has a filter function that depends on the impulse response of the ion detector. In one embodiment, the mass spectrometer includes a memory that is addressed by the register value and an adder. The adder forms the sum of the data value specified by the register value and the output value from the finite impulse response filter.

摘要翻译： 公开了具有离子加速器的质谱仪和利用有限脉冲响应滤波器的离子检测器。 离子加速器响应于起始信号产生离子脉冲。 一个时钟递增寄存器，指示从启动信号起经过的时间。 离子检测器在空间上与离子加速器分离，并产生指示离子撞击检测器的测量信号。 通过有限脉冲响应滤波器对测量信号进行滤波，形成滤波后的测量信号。 有限脉冲响应滤波器具有取决于离子检测器的脉冲响应的滤波器功能。 在一个实施例中，质谱仪包括由寄存器值寻址的存储器和加法器。 加法器形成由寄存器值指定的数据值与有限脉冲响应滤波器的输出值之和。

公开(公告)号：US20090020697A1

公开(公告)日：2009-01-22

申请号：US12242110

申请日：2008-09-30

申请人： August Hidalgo ,  John Fjeldsted ,  William Frazer

发明人： August Hidalgo ,  John Fjeldsted ,  William Frazer

IPC分类号： B01D59/44

CPC分类号： H01J49/0036

摘要： A mass spectrum is generated by a process in which, from a mass scan signal comprising original samples defining a peak, a subset of the original samples defining the peak is selected. One or more synthesized samples are synthesized from the subset of the original samples. The one or more synthesized samples provide a temporal resolution greater than the temporal resolution of the original samples. The one or more synthesized samples are summed with respective temporally-aligned accumulated samples to produce the mass spectrum. The accumulated samples are obtained from mass scan signals generated during respective previously-performed mass scan operations.

摘要翻译： 通过一个过程产生质谱，其中从包含定义峰的原始样本的质量扫描信号中选择定义峰的原始样本的子集。 从原始样品的子集合成一个或多个合成的样品。 一个或多个合成样本提供大于原始样本的时间分辨率的时间分辨率。 将一个或多个合成的样本与相应的时间对齐的累积样本相加以产生质谱。 累积的样本是从在先前执行的大量扫描操作期间产生的质量扫描信号获得的。

公开(公告)号：US20060043282A1

公开(公告)日：2006-03-02

申请号：US10927874

申请日：2004-08-27

申请人： August Hidalgo ,  Stuart Hansen ,  Gangqiang Li

发明人： August Hidalgo ,  Stuart Hansen ,  Gangqiang Li

IPC分类号： H01J49/00

CPC分类号： H01J49/401 ,  H01J49/422 ,  H01J49/424

摘要： An apparatus for analyzing ions is described. The apparatus includes an ion source, an ion trap positioned to receive ions from the ion source; a time of flight mass analyzer, and a detector operatively coupled to the time of flight. The time of flight mass analyzer includes a pulser region, and the pulser region is positioned to receive ions from the ion trap. The apparatus further includes a scanning delay timing circuit in operable relation to the pulser region. The scanning delay timing circuit is adapted to triggering an extraction pulse at the pulser region. Methods of analyzing ions by mass spectrometry are also described.

摘要翻译： 描述了用于分析离子的装置。 该装置包括离子源，定位成从离子源接收离子的离子阱; 飞行时间质量分析器，以及可操作地耦合到飞行时间的检测器。 飞行时间质量分析器包括脉冲发生器区域，并且脉冲发生器区域被定位成从离子阱接收离子。 该装置还包括与脉冲发生器区域可操作的扫描延迟定时电路。 扫描延迟定时电路适于在脉冲发生器区域触发提取脉冲。 还描述了通过质谱分析离子的方法。

公开(公告)号：US20050079505A1

公开(公告)日：2005-04-14

申请号：US10676707

申请日：2003-09-30

申请人： May Tom-Moy ,  Carl Myerholtz ,  August Hidalgo ,  William McAllister

发明人： May Tom-Moy ,  Carl Myerholtz ,  August Hidalgo ,  William McAllister

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6825 ,  C12Q1/6837 ,  Y10S435/973 ,  Y10S436/806 ,  Y10S436/809 ,  Y10S436/815 ,  Y10S436/817 ,  C12Q2565/607

摘要： Methods and apparatus for electronically addressing and interrogating microarrays are disclosed. The described microarrays include a plurality of features disposed on a substrate. Each of the features has a first electrode disposed on the substrate, a second electrode disposed on the substrate, and a probe disposed between the first electrode and second electrode. The substrate also includes addressing circuitry in operable relation to the features. Method of using the microarrays are also described.

摘要翻译： 公开了用于电子寻址和询问微阵列的方法和装置。 所描述的微阵列包括设置在基板上的多个特征。 每个特征具有设置在基板上的第一电极，设置在基板上的第二电极和设置在第一电极和第二电极之间的探针。 衬底还包括与特征可操作的寻址电路。 还描述了使用微阵列的方法。